Effect of Methyl Jasmonate on Secondary Metabolites of Sweet Basil (Ocimum basilicumL.)

Abstract

The effect of methyl jasmonate (MeJA) in terms of its induction of inherent bioactive chemicals in sweet basil (Ocimum basilicum L.) was evaluated after MeJA was sprayed on healthy basil plants. The total phenolic content of the sweet basil significantly increased after 0.1 and 0.5 mM MeJA treatments compared with the control not subjected to MeJA. Two phenolic compounds, rosmarinic acid (RA) and caffeic acid (CA), were identified as strong antioxidant constituents of the sweet basil. Their amounts also significantly increased after the MeJA treatment. In addition, eugenol and linalool increased 56 and 43%, respectively, by the 0.5 mM MeJA treatment. Due to the accumulation of RA, CA, and eugenol, which possess strong 2,2-diphenyl-1-picrylhydrazyl (DPPH*) free radical scavenging activities, the antioxidant activity of the sweet basil extract was 2.3-fold greater than that of the control after the 0.5 mM MeJA treatment. In the DPPH* assay, the EC50 values of RA, CA, and eugenol were determined as 23, 46, and 59 microM, respectively, which indicated they were 6-, 3-, and 2.4-fold more efficient than BHT (140 microM). Besides, an unidentified HPLC peak in the methanolic extract of the sweet basil was 4.3-fold higher than that of the control after the 0.5 mM MeJA treatment.

Effect of Nacl and Phenylalanine on the Production of some Secondary Metabolites in In Vitro Cultures of Mentha longifolia

“…Similar trend of results was reported by Phatak and Heble (2002), Kim et al (2006), Namdeo et al (2007) and Roy and Mukhopadhyay (2012).…”

Aroma changes of black tea prepared from methyl jasmonate treated tea plants

“…Loivamäki et al (2004) also reported that jasmonate induction led to an increase in tobacco alkaloids, phenolics, and diterpene glycosides in Nicotiana attenuate. Moreover, the impacts of MeJA on the biosynthesis of volatile compounds in climacteric and non-climacteric fruits, such as apples and strawberries, respectively, have also been reported (Ayala-Zavala et al, 2005;Kim et al, 2006;de la Peña Moreno et al, 2010a;2010b;Belhadj et al, 2008;Gohain et al, 2012). However, investigations toward the effects of exogenous MeJA on tea aroma have been scarcely reported.…”

The effect of methyl jasmonate vapors on content of phenolic compounds in seedlings of common buckwheat (Fagopyrum esculentum Moench)

“…To elicit abundant flavonoid accumulation in plant-cell cultures MJ has been often used (Fang et al 1999). Total phenolic compounds content increased after MJ treatments of romaine lettuce (Lactuca sativa L.) and sweet basil (Ocimum basilicum L.) (Kim et al 2006;Kim et al 2007). Authors of these studies explain higher accumulation of phenolics due to induction by MJ of phenylalanine ammonia-lyase (PAL) activity.…”