Effect of methyl jasmonate and salicylic acid on the production of metabolites in cell suspensions cultures of Piper cumanense (Piperaceae)

Rodríguez-Sánchez, Laura Katherine; Pérez-Bernal, Jorge Enrique; Santamaría-Torres, Mary Andrea; Marquínez, Xavier; Suárez, Luís Enríque Cuca; Prieto, Juliet A.; Patiño-Ladino, Oscar J.

doi:10.1016/j.btre.2020.e00559

Cited by 11 publications

(5 citation statements)

References 25 publications

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“…According to the culture method, Wang et al (2022) recently observed that the MeJA-treated Chinese chives had substantial variations in primary and secondary metabolites. Similar outcomes were also reported by other researchers, who claimed that using MeJA as an elicitor in the culture increased the synthesis of the secondary metabolites (Rodrıǵuez-Sańchez et al, 2020;Deveci et al, 2022;Wang et al, 2022).…”

Section: Comparison Of Steroidal Saponin Contentsupporting

confidence: 89%

In vitro production of steroidal saponin, total phenols and antioxidant activity in callus suspension culture of Paris polyphylla Smith: an important Himalayan medicinal plant

et al. 2023

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Paris polyphylla Smith (Melanthiaceae) family, which is native to the Himalayan region, has received a lot of attention recently due to its extensive history of usage in traditional medicine. The production of steroidal saponin from callus suspension cultures of P. polyphylla was observed in the current study. The current study attempted to develop a P. polyphylla plant callus suspension culture through optimization of cultivation technique for callus suspension, quantification of total phenolic components and estimation of the extract’s antioxidant activity. A light-yellow callus was formed within six weeks of cultivating rhizomes on Murashige and Skoog (MS) media supplemented with Thidiazuron (TDZ). Furthermore, the effect of TDZ, Methyl Jasmonate (MeJA), and Yeast Extract (YE) on callus growth, steroidal saponin (dioscin and diosgenin), total phenolic content, total flavonoids, total tannin, and total antioxidant activity was also measured. The medium containing 0.5 μM TDZ depicted the maximum callus biomass (2.98 g fresh weight). Significantly high phenolic and tannin content was observed in the MS medium containing 50 μM MeJA, whereas, no significant increase was observed in total tannin production in any treatment. Three in vitro assays, DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis (3-ethylbenzothiazoline- 6-sulfonic acid)) and FRAP (ferric ion reducing antioxidant potential) and FC (Folin-Ciocalteu), were used to assess antioxidant potential of callus. Maximum antioxidant analysis reported in 1.0 μM TDZ (6.89 mM AAE/100 g) containing medium followed by 50 μM MeJA (6.44 mM AAE/100 g). The HPLC analysis showed a high presence of dioscin and diosgenin (5.43% and 21.09%, respectively) compared to the wild sample (2.56% and 15.05%, respectively). According to the results, callus produced on media supplemented with 50 μM MeJA have significant phenolic contents and elevated antioxidant activity; nevertheless, callus growth was greater in the presence of 0.5 μM TDZ. The findings of the current study have commercial implications since greater biomass production will result in active phytochemicals that the pharmaceutical and nutraceutical sectors are in need desperately.

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Section: Comparison Of Steroidal Saponin Contentsupporting

confidence: 89%

In vitro production of steroidal saponin, total phenols and antioxidant activity in callus suspension culture of Paris polyphylla Smith: an important Himalayan medicinal plant

et al. 2023

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“… Hybanthus enneaspermus L-3,4-dihydroxyphenylalanine SA adventitious root Sathish et al 2020 20. Piper cumanense 5-hidroximetilfurfural, (Z)-9-octadecenamide and phenol SA and MeJA Cell suspension Rodriguez-Sanchez et al 2020 21. Scutellaria bornmuelleri Chrysin, baicalein, wogonin MeJA hairy root Gharari et al 2020a 22.…”

Section: Effect Of Meja and Sa As Elicitors On The Biosynthesis Of Sm...mentioning

confidence: 99%

Methyl jasmonate and salicylic acid as powerful elicitors for enhancing the production of secondary metabolites in medicinal plants: an updated review

Jeyasri

Muthuramalingam

Karthick

et al. 2023

Plant Cell Tiss Organ Cult

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Plant secondary metabolites are bioactive scaffolds that are crucial for plant survival in the environment and to maintain a defense mechanism from predators. These compounds are generally present in plants at a minimal level and interestingly, they are found to have a wide variety of therapeutic values for humans. Several medicinal plants are used for pharmaceutical purposes due to their affordability, fewer adverse effects, and vital role in traditional remedies. Owing to this reason, these plants are exploited at a high range worldwide and therefore many medicinal plants are on the threatened list. There is a need of the hour to tackle this major problem, one effective approach called elicitation can be used to enhance the level of existing and novel plant bioactive compounds using different types of elicitors namely biotic and abiotic. This process can be generally achieved by in vitro and in vivo experiments. The current comprehensive review provides an overview of biotic and abiotic elicitation strategies used in medicinal plants, as well as their effects on secondary metabolites enhancement. Further, this review mainly deals with the enhancement of biomass and biosynthesis of different bioactive compounds by methyl jasmonate (MeJA) and salicylic acid (SA) as elicitors of wide medicinal plants in in vitro by using different cultures. The present review was suggested as a significant groundwork for peers working with medicinal plants by applying elicitation strategies along with advanced biotechnological approaches.

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“…Asam jasmonat berupa senyawa alami yang disintesis tumbuhan sebagai respon resistensi terhadap serangan patogen dan kerusakan eksternal (Geupil et al, 2020;Torres et al, 2020). Asam jasmonat dapat menginduksi pembukaan stomata, dan berpengaruh pada penyerapan nitrogen dan fosfor, dan transport senyawa organik seperti glukosa (Gupta et al, 2017).…”

Section: Pendahuluanunclassified

“…Asam jasmonat adalah senyawa alami yang disintesis tumbuhan sebagai respon resistensi terhadap serangan patogen dan kerusakan eksternal (Gomi, 2020;Torres et al, 2020). Sebagai regulator seluler, metil jasmonat dapat memacuh perkembangan akar, pembentukan umbi, perkembangan bunga, perkecambahan biji.…”

Section: Metode Pelaksanaanunclassified

PKM Kelompok Tani Tiga Berlian Kelurahan Paslaten Dua Kota Tomohon, tentang Penggunaan Elisitor Methyl Jasmonat dalam Budidaya Tanaman Sayuran

Rampe,

Pontororing,

Rampe

2023

TSSS

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Methyl jasmonat sebagai elisitor kimia dapat menginduksi resistensi tanaman terhadap serangga hama herbivora melalui produksi metabolit sekunder. Metil jasmonat dapat memodulasi proses fisiologis tumbuhan dalam kondisi cekaman abiotik. Kelurahan Paslaten Satu Kota Tomohon sebagian penduduknya berprofesi sebagai petani. Untuk meningkatkan produksi dan menekan adanya hama dan penyakit pada tanaman pertanian, umumnya para petani menggunakan pestisida kimia yang berdampak negatif bagi lingkungan dan terjadinya akumulasi pestisida pada produk pertanian. Salah satu strategi untuk mengurangi penggunaan pestisida kimia adalah dengan menerapkan konsep pertanian tanpa pestisida, dengan cara menginduksi resistensi tanaman menggunakan elisitor methyl jasmonat. Kegiatan PKM dilaksanakan dengan tahapan : 1) Pemberian teori/ceramah, 2) Praktek pembuatan larutan methyl jasmonat, 3) Praktek aplikasi larutan methyl jasmonat pada tanaman sayuran, 4) pendampingan pada peserta kelompok tani 5) Evaluasi dan monitoring. Kontribusi dan target khusus yang ingin dicapai adalah peningkatan pengetahuan dan ketrampilan dalam pemanfaatan elisitor methyl jasmonat yang memberikan dampak positif yaitu perobahan perilaku ke pola pertanian tanpa pestisida kimia, mengurangi dampak negatif pestisida, dan dapat meneruskan kepada para petani yang belum menjadi peserta penyuluhan. Tujuan dan target khusus yang ingin dicapai adalah peningkatan pengetahuan mitra, perobahan perilaku ke pertanian tanpa pestisida kimia, dengan memanfaatkan elisitor methyl jasmonat. Kegiatan PKM tentang penggunaan elisitor methyl jasmonat dalam budidaya tanaman sayuran memberikan dampak positif bagi petani berupa pengetahuan dan ketrampilan tentang aplikasi elisitor methyl jasmonat pada tanaman sayuran.

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Effect of methyl jasmonate and salicylic acid on the production of metabolites in cell suspensions cultures of Piper cumanense (Piperaceae)

Cited by 11 publications

References 25 publications

In vitro production of steroidal saponin, total phenols and antioxidant activity in callus suspension culture of Paris polyphylla Smith: an important Himalayan medicinal plant

In vitro production of steroidal saponin, total phenols and antioxidant activity in callus suspension culture of Paris polyphylla Smith: an important Himalayan medicinal plant

Methyl jasmonate and salicylic acid as powerful elicitors for enhancing the production of secondary metabolites in medicinal plants: an updated review

PKM Kelompok Tani Tiga Berlian Kelurahan Paslaten Dua Kota Tomohon, tentang Penggunaan Elisitor Methyl Jasmonat dalam Budidaya Tanaman Sayuran

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