Effect of Methyl Ester of Jasmonic Acid and Benzylaminopurine on Growth and Protein Profile of Excised Cotyledons of Cucurbita Pepo (Zucchini)

Ananieva, Kalina; Ananiev, E. D.

doi:10.1023/a:1002615115118

Cited by 17 publications

(10 citation statements)

References 24 publications

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“…MeJA leads to the suppression of total protein and RNA synthesis as well as the activity of nuclear RNA polymerases [85]. 40S ribosomal protein S12-1 (spot 38), TCP-1/cpn60 chaperonin family protein (spot 799) and ATP-dependent Clp protease proteolytic subunit 2 (spot 178) were decreased in MeJA-treated jin1-9/myc2 plants compared to WT plants (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Proteomic identification of MYC2-dependent jasmonate-regulated proteins in Arabidopsis thaliana

et al. 2012

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BackgroundMYC2, a basic helix-loop-helix (bHLH) domain-containing transcription factor, participates in the jasmonate (JA) signaling pathway and is involved in the modulation of diverse JA functions. However, a comprehensive list of MYC2-dependent JA-responsive proteins has yet to be defined.ResultsIn this paper, we report the comparative proteomics of wild-type (WT) plants and jin1-9, a MYC2 mutant plant, in response to methyl jasmonate (MeJA) treatment. Proteins from mock/MeJA-treated jin1-9 and WT samples were extracted and separated by two-dimensional gel electrophoresis. Twenty-seven JA-mediated proteins demonstrated differential expression modulated by MYC2. We observed that MYC2 negatively regulates the accumulation of JA-dependent indolic glucosinolate-related proteins and exhibits opposite effects on the biosynthetic enzymes involved aliphatic glucosinolate pathways. In addition, proteins involved in the tricarboxylic acid cycle and a majority of the MeJA-inducible proteins that are involved in multiple protective systems against oxidative stress were reduced in jin1-9/myc2 sample compared to the WT sample. These results support a positive role for MYC2 in regulating JA-mediated carbohydrate metabolism and oxidative stress tolerance.ConclusionsWe have identified MYC2-dependent jasmonate-regulated proteins in Arabidopsis thaliana by performing two-dimensional gel electrophoresis and MALDI-TOF/TOF MS analysis. The observed pattern of protein expression suggests that MYC2 has opposite effects on the biosynthetic enzymes of indolic and aliphatic glucosinolate pathways and positively regulates JA-mediated carbohydrate metabolism and oxidative stress tolerance-related proteins. Furthermore, it is very interesting to note that MYC2 plays opposite roles in the modulation of a subset of JA-regulated photosynthetic proteins during short-term and long-term JA signaling. This study will enhance our understanding of the function of MYC2 in JA signaling in Arabidopsis thaliana.

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Section: Resultsmentioning

confidence: 99%

Proteomic identification of MYC2-dependent jasmonate-regulated proteins in Arabidopsis thaliana

et al. 2012

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“…The reason for the difference between radish and zucchini is not clear, but Ueda & Kato (1982) used light‐grown material and treated the cotyledons several days after germination, whereas we used etiolated material and treated cotyledons immediately after excision from dry seeds. Sensitivity of zucchini cotyledons to jasmonic acid may change with time from germination insofar as fresh weight gain in cotyledons excised 4 day after germination was insensitive to jasmonate (Ananieva & Ananiev 1999) whereas cotyledons excised from dry seeds responded (Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…Because cotyledon expansion initially requires stored reserves that might otherwise be used for other seedling organs, it is possible that this expansion is subject to a complicated net of hormonal interactions, tuning growth to the environment. While cytokinin has long been known as the major, hormonal regulator of cotyledon development (reviewed in Mok & Mok 2001), there is evidence that jasmonic acid and its methyl ester may also be involved, possibly as antagonists to cytokinin (Ueda & Kato 1982; Ananieva & Ananiev 1999; Mukherjee et al. 2002).…”

Section: Introductionmentioning

confidence: 99%

Differential effects of methyl jasmonate on growth and division of etiolated zucchini cotyledons

et al. 2008

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The jasmonates are well studied in the context of plant defence but increasingly are also recognised as playing roles in development. In many systems, jasmonates antagonise the effects of cytokinins. The aim of the present work was to elucidate interactions between methyl jasmonate and cytokinin (benzyladenine) in regulating growth of zucchini (Cucurbita pepo L., cv. Cocozelle, var. Tripolis) cotyledons, taking advantage of the ability to simultaneously quantify cell enlargement and division from paradermal sections of the first palisade layer. Growth regulators were applied to cotyledons, excised from dry seeds and grown in darkness. Cytokinin stimulated expansion and division whereas, surprisingly, jasmonate stimulated expansion but inhibited division. Jasmonate antagonised the stimulating effect of cytokinin on division but worked cooperatively with cytokinin in increasing expansion. However, expansion with jasmonate was more isotropic than with cytokinin. Jasmonate also stimulated the loss of cellular inclusions and soluble protein. Soluble proteins revealed a partial antagonism between jasmonate and cytokinin. These results illustrate the complex interplay between jasmonates and cytokinin in the regulatory network of cotyledon development following germination.

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“…Other components of the signal network are the induction of phospholipases (PLPs), which act on lipidbound unsaturated fatty acids within the membrane, resulting in the releasing of jasmonate, methyl jasmonate and related molecules. Recent evidence confirmed the potential role of jasmonate, ethylene and salicylic acid in the signal pathways leading to up-regulation of pathogen defense-related genes in plants (Ananieva and Ananiev, 1999;Reymond and Farmer, 1998).…”

Section: Hypersensitive Cell Death and Defense Mechanismmentioning

confidence: 78%

The molecular initiation and subsequent acquisition of disease resistance in plants

Gachomo¹,

Shonukan²,

Kotchoni³

2003

Afr. J. Biotechnol.

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Interactions between disease resistance (R) genes in plants and their corresponding pathogen avirulence (Avr) genes are the key determinants of whether a plant is susceptible or resistance to a pathogen attack. Evidence has emerged that these gene-for-gene interactions in the perception of pathogenic invasions and development of acquired resistance in plants involve different molecular and hormonal transduction pathways, which are still poorly understood. It has become apparent that plants actively produce several phytohormones such as ethylene, jasmonate, salicylic acid, and reactive oxygen intermediates prior to upregulation of R genes. The physiological role of these molecules in plant resistance to pathogens is beginning to attract attention. The use of transgenic plants in recent attempts, including development of mutants with altered R genes, has provided new insights into the mechanisms involved in pathogen perception, signal transduction and subsequent resistance to disease in plants. This review tries to summarize current knowledge of pathogen-related genes in plants, and how they can be use to improve disease resistance in agronomically valuable plants. It also describes the molecular basis of defense mechanisms in plants under pathogen attack.

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Effect of Methyl Ester of Jasmonic Acid and Benzylaminopurine on Growth and Protein Profile of Excised Cotyledons of Cucurbita Pepo (Zucchini)

Cited by 17 publications

References 24 publications

Proteomic identification of MYC2-dependent jasmonate-regulated proteins in Arabidopsis thaliana

Proteomic identification of MYC2-dependent jasmonate-regulated proteins in Arabidopsis thaliana

Differential effects of methyl jasmonate on growth and division of etiolated zucchini cotyledons

The molecular initiation and subsequent acquisition of disease resistance in plants

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