Effect of Methionine Hydroxy Analog on Bacterial Protein Synthesis from Urea and Glucose, Starch or Cellulose by Rumen Microbes, In vitro

Gil, L.; Shirley, R. L.; Moore, John E.

doi:10.2527/jas1973.371159x

Cited by 27 publications

(19 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This lack of lactation response to MHA is in contrast to results with beef cows (Varner et al, 1973) and dairy cows . These workers combined MHA with natural protein, but MHA significantly increased bacterial nitrogen and cellulose digestion, and lowered ammonia levels with urea in vitro (Gil et al,, 1973).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Beef Cattle Range Supplements Containing Urea and Biuret2

Rush

Johnson

Totusek

1976

Journal of Animal Science

View full text Add to dashboard Cite

SUMMARYTwo winter trials were conducted with 304 lactating range cows on dry grass to evaluate non-protein-nitrogen (NPN) in 30% protein supplements containing biuret (pure and feed grade), urea and extruded grain-urea. The NPN sources contributed one-half of the supplemental nitrogen with natural 15 and 30% protein supplements serving as negative and positive controls.Winter weight loss of cows was greater (P~.02) on the negative than on the positive control in both trials. The apparent utilization of all NPN sources was low and the utilization of urea and extruded grain-urea was less than pure or feed grade biuret. Rumen biuretolytic activity was apparent within 6 days and reached and maintained a high level of activity 20 days after the initiation of feeding biuret, even with intermittent supplementation. Apparent value of NPN supplements was slightly improved with 40% dehydrated alfalfa but not with methionine-hydroxy-analogue (MHA). Palatability of supplements was lowered by urea and especially

show abstract

Section: Resultsmentioning

confidence: 99%

Evaluation of Beef Cattle Range Supplements Containing Urea and Biuret2

Rush

Johnson

Totusek

1976

Journal of Animal Science

View full text Add to dashboard Cite

show abstract

“…reported that rate of digestion of a mature forage with urea-based supplements was increased with methionine compared with ammonium sulfate, but calculated ruminal digestibilities did not differ between the two treatments. Increases in urea utilization and cellulose digestion with methionine compared with inorganic sulfur sources observed in vitro (Hunt et al, 1954;Gil et al, 1973;Spears et al, 1976) do not seem to increase in vivo DM digestibility.…”

Section: Resultsmentioning

confidence: 74%

Effect of methionine addition to a urea-grain supplement on intake and digestibility of mature, dormant grasses and performance of cows grazing winter range

Momont

Pruitt

Johnson

1993

Journal of Animal Science

View full text Add to dashboard Cite

A digestibility trial involving 20 Hampshire ram lambs and a 2-yr grazing study using 103 mature crossbred cows were conducted to determine the effects of methionine addition to a urea-grain supplement on intake and digestibility of dormant range grasses and on cow performance. In each trial, four treatment groups were supplemented with either a urea-grain control (CON), urea-grain plus methionine (MET, 3.3% DL-methionine), urea-grain plus inorganic sulfur (SUL, 3.0% sodium sulfate), or soybean meal (SBM). Supplements were designed to provide 45 and 360 g of CP.animal-1.d-1 (lambs and cows, respectively) and were balanced for ME, Ca, P, and K. Lambs had ad libitum access to mature prairie hay, whereas cows grazed dormant winter range from mid-November until mid-February. For the grazing study, forage OM intake (OMI) was determined in late November and in late January by the fecal output/indigestibility ratio technique. Controlled-release chromic oxide boluses were used as an external marker to estimate fecal output, and acid insoluble ash was used as an internal marker to predict OM digestibility (OMD). Mean daily DMI of mature prairie hay was 1,057 g/lamb and was not affected by supplementation. Apparent DM, NDF, and ADF digestibilities and N biological value did not differ (P > .10) among treatments. Nitrogen digestibility was increased (P = .06) for lambs fed the MET or SUL compared with CON.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

“…The degree to which methionine is used as an intact amino acid versus being degraded and used as a sulfur source by cellulolytic microbes in the rumen remains unclear. Numerous researchers (Hunt et al 1954;Gil et al 1973Spears et al 1976 have demonstrated that methionine when used as a sulfur source increases cellulose digestion. Maeng et al (1976) suggested that sulfur amino acids were utilized intact and improved in vitro microbial cell yields with cellobiose as the substrate.…”

Section: Resultsmentioning

confidence: 99%

Effects of Buffer Additions on Fermentation of Dormant Range Grasses

Momont¹,

Pruitt²,

Pritchard³

et al. 1995

Journal of Range Management

View full text Add to dashboard Cite

Replicated two-stage in vitro studies were conducted to determine the effects of single amino acid or branched-chain volatile fatty acid buffer additions on in vitro dry matter disappearance, neutral detergent fiber disappearance, and fermentation kinetics of dormant range grasses. Substrates consisted of separate samples of 2 cool season mid-grasses, western wheatgrass (Agropyron smithii Rydb.) and Japanese brome (Bromus japonicus Thunb.), and a misture of warm season shortgrasses, buffalograss (Bunchloe dactyloids [Nutt.] Engelm.), and blue grama (Bouteloua gracilis [H.B.K.] Lag. ex Steud.), hand clipped from standing forage in mid-hlarch. Isonitrogenous treatments included buffer containing urea with or without amino acids, branched-chain volatile fatty acids, sodium sulfate, or starch. Urea increased (P

show abstract

Effect of Methionine Hydroxy Analog on Bacterial Protein Synthesis from Urea and Glucose, Starch or Cellulose by Rumen Microbes, In vitro

Cited by 27 publications

References 0 publications

Evaluation of Beef Cattle Range Supplements Containing Urea and Biuret2

Evaluation of Beef Cattle Range Supplements Containing Urea and Biuret2

Effect of methionine addition to a urea-grain supplement on intake and digestibility of mature, dormant grasses and performance of cows grazing winter range

Effects of Buffer Additions on Fermentation of Dormant Range Grasses

Contact Info

Product

Resources

About