Effect of methanol on the frequency of respiration-deficient mutation induced by ethidium bromide in yeast under growing and non-growing conditions.

Hamada, Hiroshi; Yamashita, Miyuki; Kashige, Nobuhiro; Kojima, Misao; Morita, Tamotsu

doi:10.1248/cpb.36.4865

Cited by 3 publications

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“…Overall judgement: The methods implemented were thought not to be sufficiently robust to support the results reported. Ref: Hamada et al (1988) Opinion on the re-evaluation of aspartame (E951) as a food additive EFSA Journal 2013;11(12):3496 219…”

Section: Negative B)mentioning

confidence: 99%

“…The Panel considered that the methods implemented were sufficiently robust to support the results reported. Hamada et al (1988) examined methanol (2-8 %; 0.49-1.97 M)) for induction of gene mutations in Saccharomyces cerevisiae ATCC26422 as a control culture within a study which aimed at elucidating the effects of ethidium bromide. Methanol did not induce gene mutations, but was only tested without a metabolic activation system.…”

Section: In Vitro Studiesmentioning

confidence: 99%

“…Overall judgement: The methods implemented were thought not to be sufficiently robust to support the results reported. Ref: Hamada et al (1988) EFSA The highest dose may also be defined as a dose that produces some indication of toxicity in the bone marrow (e.g. greater than 50 % reduction in mitotic index).…”

Section: Identificationmentioning

confidence: 99%

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Scientific Opinion on the re‐evaluation of aspartame (E 951) as a food additive

2013

EFS2

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The EFSA ANS Panel provides a scientific opinion on the safety of aspartame (E 951). Aspartame is a sweetener authorised as a food additive in the EU. In previous evaluations by JECFA and the SCF, an ADI of 40 mg/kg bw/day was established based on chronic toxicity in animals. Original reports, previous evaluations, additional literature and data made available following a public call were evaluated. Aspartame is rapidly and completely hydrolysed in the gastrointestinal tract to phenylalanine, aspartic acid and methanol. Chronic and developmental toxicities were relevant endpoints in the animal database. From chronic toxicity studies in animals, a NOAEL of 4000 mg/kg bw/day was identified. The possibility of developmental toxicity occurring at lower doses than 4000 mg/kg in animals could not be excluded. Based on MoA and weight‐of‐evidence analysis, the Panel concluded that developmental toxicity in animals was attributable to phenylalanine. Phenylalanine at high plasma levels is known to cause developmental toxicity in humans. The Panel concluded that human data on developmental toxicity were more appropriate for the risk assessment. Concentration‐response modelling was used to determine the effects of aspartame administration on plasma phenylalanine using human data after phenylalanine administration to normal, PKU heterozygote or PKU homozygote individuals. In normal and PKU heterozygotes, aspartame intakes up to the ADI of 40 mg/kg bw/day, in addition to dietary phenylalanine, would not lead to peak plasma phenylalanine concentrations above the current clinical guideline for the prevention of adverse effects in fetuses. The Panel concluded that aspartame was not of safety concern at the current aspartame exposure estimates or at the ADI of 40 mg/kg bw/day. Therefore, there was no reason to revise the ADI of aspartame. Current exposures to aspartame ‐ and its degradation product DKP ‐ were below their respective ADIs. The ADI is not applicable to PKU patients.

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Section: Negative B)mentioning

confidence: 99%

Section: In Vitro Studiesmentioning

confidence: 99%

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Scientific Opinion on the re‐evaluation of aspartame (E 951) as a food additive

2013

EFS2

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Methanol [MAK Value Documentation in German language, 1999]

2012

The MAK‐Collection for Occupational Health and Safety

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Veröffentlicht in der Reihe Gesundheitsschädliche Arbeitsstoffe , 29. Lieferung, Ausgabe 1999 Der Artikel enthält folgende Kapitel: Allgemeiner Wirkungscharakter Wirkungsmechanismus Toxikokinetik und Metabolismus Erfahrungen beim Menschen Einmalige Exposition Tierexperimentelle Befunde und In‐vitro‐Untersuchungen Bewertung

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Methanol [MAK Value Documentation, 2001]

2012

The MAK‐Collection for Occupational Health and Safety

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Published in the series Occupational Toxicants , Vol. 16 (2001) The article contains sections titled: Toxic Effects and Mode of Action Mechanism of Action Toxicokinetics and Metabolism Absorption and distribution Metabolism and excretion Levels of methanol and formate in blood and urine Effects in Man Single exposures Repeated exposure Local effects on skin and mucous membranes Allergenic effects Reproductive toxicity Genotoxicity Carcinogenicity Animal Experiments and in vitro Studies Acute toxicity Subacute, subchronic and chronic toxicity Local effects on skin and mucous membranes Allergenic effects Reproductive and developmental toxicity Genotoxicity Carcinogenicity Manifesto

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Effect of methanol on the frequency of respiration-deficient mutation induced by ethidium bromide in yeast under growing and non-growing conditions.

Abstract: The effect of methanol on the frequency of cytoplasmic respiration-deficient(RD)mutation

Cited by 3 publications

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Scientific Opinion on the re‐evaluation of aspartame (E 951) as a food additive

Scientific Opinion on the re‐evaluation of aspartame (E 951) as a food additive

Methanol [MAK Value Documentation in German language, 1999]

Methanol [MAK Value Documentation, 2001]

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