Effect of Methanol and water contents on production of biodiesel fuel from plant oil catalyzed by various lipases in a solvent-free system

Kaieda, Masaru; Samukawa, Taichi; Kondo, Akihiko; Fukuda, Hideki

doi:10.1016/s1389-1723(01)80103-1

Cited by 195 publications

(35 citation statements)

References 7 publications

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“…A similar trend was observed for the reaction with methanol, except that the reaction rate did not change as dramatically as that with ethanol. The highest enzyme activity was observed at water content of 5% (v/v) for methanol, the same optimal water content as reported in a study of methanolysis activities of different lipases for synthesis of biodiesel from soybean oil [9].…”

Section: Transesterification Reactions With Different Lipasessupporting

confidence: 83%

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An Organic Soluble Lipase for Water-Free Synthesis of Biodiesel

Zhao

El‐Zahab

Brosnahan

et al. 2007

Appl Biochem Biotechnol

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Lipase AK was modified with short alkyl chains to form a highly organic soluble enzyme and was used to catalyze the synthesis of biodiesel from soybean oil in organic media. The effects of several key factors including water content, temperature, and solvent were examined for the solubilized enzyme in comparison with several other commercially available lipases. Whereas native lipases showed no activity in the absence of water, the organic soluble lipase demonstrated reaction rates of up to 33 g-product/genzyme h. The biocatalyst remains soluble in the biodiesel product, and therefore, there is no need to be removed because it is expected to be burned along with the diesel in combustion engines. This provides a promising one-pot mix-and-use strategy for biodiesel production.

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Section: Transesterification Reactions With Different Lipasessupporting

confidence: 83%

“…Several recently reported works have examined the influence of water on the transesterification activities of lipases. According to these works, a water content of at least 0.48% (wt) is required to activate the enzymes [5,[8][9][10]. That is about ten times of the allowed 0.05% water content for biodiesel in the USA [11].…”

mentioning

confidence: 99%

An Organic Soluble Lipase for Water-Free Synthesis of Biodiesel

Zhao

El‐Zahab

Brosnahan

et al. 2007

Appl Biochem Biotechnol

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“…This is illustrated by the results of Noureddini et al (2005), Kaieda et al (2001), and Hernández-Martín and Otero (2008).…”

Section: Acyl Acceptorsmentioning

confidence: 81%

“…Optimal water activity for the enzymatic transesterification reaction system including substrates, enzymes, solvent and possibly also carrier material is specific for a given lipase, as seen from the results of Kaieda et al (2001), Linko et al (1995), Tan et al (2006), Zhang et al (2002Zhang et al ( , 2005, and Lu et al (2008). Kaieda et al (2001) tested free lipases from C. rugosa, P. cepacia, and P. fluorescens. If the system was water free, no reaction took place while the rate of reaction increased with increased water content (1-20 wt% water).…”

Section: Transesterification With Enzymes Choice Of Enzymesmentioning

confidence: 99%

A review of the current state of biodiesel production using enzymatic transesterification

Fjerbæk

Christensen

Norddahl

2009

Biotech & Bioengineering

662

459

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Enzymatic biodiesel production has been investigated intensively, but is presently employed industrially only in a 20,000 tons/year pilot plant in China (Du et al. [2008] Appl Microbiol Technol 79(3):331-337). This review presents a critical analysis of the current status of research in this area and accentuates the main obstacles to the widespread use of enzymes for commercial biodiesel transesterification. Improved results for enzymatic catalysis are seen with respect to increased yield, reaction time and stability, but the performance and price of the enzymes need further advances for them to become attractive industrially for biodiesel production. Critical aspects such as mass transfer limitations, use of solvents and water activity are discussed together with process considerations and evaluation of possible reactor configurations, if industrial production with enzymes is to be carried out. Results of published studies on the productivity of enzymes are also presented and compared to the use of chemical catalysts.

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“…Des études antérieures décrites par Mittelbach [19] et Basri et al [20] ont montré l'effet inhibiteur du méthanol sur certaines souches de lipases, notamment en absence de solvant organique. Néanmoins, les lipases des souches de Pseudomonas résistent aux fortes teneurs en méthanol [21,22]. Selon certains auteurs, la méthano-lyse devient favorable lorsque le méthanol se trouve complètement dissout dans le milieu réactionnel [23,24].…”

Section: Alcoolyse Enzymatique Des Huiles Végétalesunclassified