Effect of metformin and adriamycin on transplantable tumor model

Kabel, Ahmed M.; Omar, Mohamed S.; Balaha, Mohamed; Borg, Hany M.

doi:10.1016/j.tice.2015.07.003

Cited by 16 publications

(8 citation statements)

References 56 publications

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“…This indicates that ADM and MET in combination is more effective than ADM and MET alone in inhibiting the proliferation of tumor cells. It was also found that the combination of MET and ADM has a more significant effect on Ehrlich tumors in mice than ADM or MET alone (22). It was found that the apoptosis rate in the ADM, MET and ADM+MET groups was significantly higher than that in the control group (P<0.05).…”

Section: Discussionmentioning

confidence: 99%

Effect of adriamycin combined with metformin on biological function of human tongue cancer SSC-15 cells

Zhang

2019

Oncol Lett

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The effect of adriamycin (ADM) combined with metformin (MET) on the biological function of human tongue cancer SSC-15 cells was investigated. SCC-15 cells (ATCC ® CRL-1623) were cultured in vitro . The close concentration of the median lethal dose (LD50) of ADM was 0.05 mg/l and the LD50 of MET was 10 mmol/l after 48 h of intervention. They were used for drug combination experiments. Cells without drug treatment were used as the control group, cells treated with ADM alone, MET alone and their drug combination (ADM+MET) as the experimental groups. CCK-8 was used to detect the cell survival rate, and flow cytometry to detect the apoptosis rate in each group, Transwell chamber to detect the invasion ability in vitro of cells and scratch-healing experiment to observe the migration ability of the cells. The survival rate of tongue cancer SCC-15 cells gradually decreased with the increase in ADM and MET concentrations and in intervention time (P<0.05). The apoptosis rate in the ADM, MET and ADM+MET groups was significantly higher than that in the control group (P<0.05). The apoptosis rate in the ADM+MET group was higher than that in the ADM and MET groups (P<0.05). The invasion and migration ability of cells in the ADM and MET groups were higher than those in the ADM+MET group (P<0.05). The cell membrane number and the migration rate of cells in the ADM+MET group were significantly lower than those in the ADM and MET groups (P<0.05). Both MET and ADM inhibit the growth, invasion and migration of tongue cancer SSC-15 cells, and induce their apoptosis. Thus, ADM and MET in combination is more effective than ADM alone and MET alone in inhibiting the growth, invasion and migration of tongue cancer cells as well as in inducing their apoptosis.

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Section: Discussionmentioning

confidence: 99%

Effect of adriamycin combined with metformin on biological function of human tongue cancer SSC-15 cells

Zhang

2019

Oncol Lett

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“…For the induction of SEC, the ECCs were subcutaneously injected into the left thigh of the hind limb of mice. After ten days, visible solid masses (about 100 mm 3 ) were observed [18].…”

Section: A T E R I a L S A N D M E T H O D Smentioning

confidence: 99%

Effect of dapagliflozin and/or L‐arginine on solid tumor model in mice: The interaction between nitric oxide, transforming growth factor‐beta 1, autophagy, and apoptosis

Kabel

Arab

Elmaaboud

2021

Fundamemntal Clinical Pharma

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BackgroundNitric oxide was reported to play an essential role in various physiological and pathological processes in the body. Recent reports suggested that nitric oxide may affect the pathogenesis of cancer. Dapagliflozin is a sodium‐glucose cotransporter 2 inhibitor which is commonly used for type‐2 diabetes mellitus management.PurposeThe current work aimed to detect the potential impact of dapagliflozin and/or L‐arginine on solid Ehrlich carcinoma (SEC) in mice.MethodsSix equal groups of male BALB/c mice were divided as follows: Control; SEC; SEC + Dapagliflozin; SEC + L‐arginine; SEC + carboxymethyl cellulose; and SEC + Dapagliflozin + L‐arginine group. Tumor volume, survival rate, tissue total nitrate/nitrite, paraoxonase‐1, interleukin 1 alpha (IL‐1α), and transforming growth factor‐beta 1 (TGF‐β1) were determined. Also, caspase 3, beclin‐1, and c‐Jun NH2‐terminal kinase (JNK) activities were estimated in the tumor tissues. Sections of the tumor tissues were examined by histopathology and immunohistochemistry.ResultsDapagliflozin and/or L‐arginine induced a significant increment of the survival rate, tissue total nitrate/nitrite, paraoxonase‐1, caspase 3, beclin‐1, and JNK activities, significant lowering of the tumor volume, tissue TGF‐β1, and IL‐1α expression alongside an improvement of the histopathologic findings, versus the SEC group. Notably, the combination of dapagliflozin/L‐arginine exerted more pronounced effects versus each agent alone.ConclusionDapagliflozin/L‐arginine combination may confer a novel therapeutic line for cancer therapy.

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“…Early signs of pituitary adenomas usually depend on whether the tumor is functioning (making excess hormones) or non-functioning (not making excess hormones) [17]. Functioning adenomas may cause problems because of the hormones they release.…”

Section: Clinical Presentation Of Pituitary Adenomasmentioning

confidence: 99%

Pituitary Adenomas: Insights into the Recent Trends

Kabel¹

2020

JCRT

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Pituitary adenoma is a type of pituitary neoplasms that is typically a benign, slow-growing tumor that arises from cells in the pituitary gland. Pituitary adenomas are classified according to their secretory products into functioning (Endocrine-active) tumors that represent almost 70% of pituitary tumors, and non-functioning adenomas (Endocrine-inactive). Because of physiologic effects of excess hormones, the functioning tumors present earlier than non-functioning adenomas. Patients with pituitary adenomas usually present with symptoms related to hormonal disturbances. However, pressure symptoms, such as headache, visual field defects, cranial nerve palsies and hypopituitarism may occur due to the mass effect of large tumors. Treatment of pituitary adenomas include transsphenoidal surgery and adjunctive therapy including supervoltage radiation and pharmacologic agents. This review throws light on pituitary adenoma regarding its prevalence, predisposing factors, types, clinical presentation, diagnosis and possible lines of management in view of the recent trends.

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Effect of metformin and adriamycin on transplantable tumor model

Cited by 16 publications

References 56 publications

Effect of adriamycin combined with metformin on biological function of human tongue cancer SSC-15 cells

Effect of adriamycin combined with metformin on biological function of human tongue cancer SSC-15 cells

Effect of dapagliflozin and/or L‐arginine on solid tumor model in mice: The interaction between nitric oxide, transforming growth factor‐beta 1, autophagy, and apoptosis

Pituitary Adenomas: Insights into the Recent Trends

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