Effect of Metarhizium anisopliae (Ascomycete), Cypermethrin, and D-Limonene, Alone and Combined, on Larval Mortality of Rhipicephalus sanguineus (Acari: Ixodidae)

Prado-Rebolledo, Omar; Molina-Ochoa, Jaime; Lezama-Gutiérrez, Roberto; García-Márquez, Luis Jorge; Minchaca-Llerenas, Yureida B; Morales-Barrera, Eduardo; Téllez, G.; Hargis, Billy M.; Skoda, S.; Foster, John E.

doi:10.1093/jme/tjx092

Cited by 9 publications

(4 citation statements)

References 22 publications

(21 reference statements)

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“…The present study shows, for the first time, that a native strain of M. anisopliae was found highly virulent towards R. sanguineus eggs. Metarhizium anisopliae has long been studied on its efficacy towards different species of soft and hard ticks (Gindin et al, 2009;Ren et al, 2016;Fernandez-Salas et al, 2017;Fischhoff et al, 2017;Prado-Rebolledo et al, 29.57 ± 4.82b 10 6 conidia mL -1 30.22 ± 4.90b 10 7 conidia mL -1 30.22 ± 4.90b…”

Section: Resultsmentioning

confidence: 99%

Ovicidal Efficacy of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) towards Rhipicephalus sanguineus (Acari: Ixodidae) Eggs

et al. 2021

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Rhipicephalus sanguineus, commonly known as brown dog tick is a widespread species with considerable public health and economic importance. Tremendous efforts were performed to control the tick populations with the concern of resistance build-up and environmental issues. Alternative towards microbial control thus emerged as one option to reduce tick populations. In this study, the ovicidal efficacy of a native isolate entomopathogenic hyphomycetes fungi, Metarhizium anisopliae strain HSAH5 was evaluated against eggs of R. sanguineus. Spray applications with three different conidial concentrations of 10 5 , 10 6 and 10 7 conidia mL-1 ; 40 ppm of Flumethrin and a negative control. The M. anisopliae strain was found highly virulent to R. sanguineus eggs by reducing the hatching percentages to ≈30% compared with 8.9% in Flumethrin eggs. The result showed a significantly higher mortality in M. anisopliae group than those of the control groups (F = 42.08, df = 32, P < 0.001) at 30 days post-infection. However, there are no significant differences within the M. anisopliae group, in which the mortality between different conidial concentrations is almost the same. The estimated LC 50 of M. anisopliae against eggs of R. sanguineus is 1.36 × 10 3 conidia ml-1. Thus, these results suggest M. anisopliae strain HSAH5 could be a potential biocontrol agent of R. sanguineus in the integrated approach to managing ticks in the residential landscape by targeting on the eggs.

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Section: Resultsmentioning

confidence: 99%

Ovicidal Efficacy of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) towards Rhipicephalus sanguineus (Acari: Ixodidae) Eggs

et al. 2021

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“…It is reported that low concentrations of D-limonene can kill Aonidiella aurantii [139], the LC 50 for adult Sitophilus oryzae was 36.85 µL/L [140], and the percentage of death in Rhipicephalus sanguineus treated with D-limonene at 0.1 µL/L was 82.6% [141]. The use of oil-in-water nanoemulsions made of D-limonene (8.9% w/w), water (30.2% w/w), Tween 20 (36.9% w/w), propylene glycol (15.1% w/w), and ethanol (8.9% w/w) were highly toxic to larvae of Culex pipiens molestus and Aedes albopictus and the toxicity effect was stronger than that of the unencapsulated D-limonene solution; this is probably due to the increased surface to volume ratio of nanoencapsulated D-limonene, which is beneficial to penetration, making it easier to pass through the insect's exoskeleton and protect the active substances within it from inactivation and degradation.…”

Section: Anthelmintic and Insecticidal Activitymentioning

confidence: 99%

D-Limonene: Promising and Sustainable Natural Bioactive Compound

Lin,

Li,

Sun

et al. 2024

Applied Sciences

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The discovery of antibiotics and pesticides has greatly contributed to the social and economic development of human society but, due to the long-term irrational application, it has led to drug-resistant microorganisms, environmental damage, and other hazards, so the selection of alternative natural, safe, and non-hazardous bioactive substances is an effective solution for this problem. D-limonene is a bioactive compound widely present in various plant essential oils, exhibiting excellent broad-spectrum bioactivity and promising prospects for development and clinical application. This review provides a detailed overview of the biological activities of D-limonene, emphasizing its antimicrobial, anthelmintic, insecticidal, and medicinal potential. While nanoencapsulation technology shows promise in improving the physicochemical properties of D-limonene and enhancing its practical applications, it is also crucial to comprehensively evaluate the potential side effects of D-limonene before use.

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“…The mycoacaricide activity was enhanced, when fungal stages were added with deltamethrin to control pyrethroid-resistant R. microplus [171,172] or with cypermethrin and chlopyriphos [173]. Similarly, I. scapularis was sensitive to fipronil, added to M. anisopliae [174,175], as well as R. sanguineus to M. anisopliae plus cypermethrin [176]. An integrated alternative control of R. microplus by both essential oils and entomopathogenic fungi (M. anisopliae and B. bassiana) indicated the suitability of Pelargonium graveolens essential oil that is not able to inhibit these EPFs and, when B. bassiana is not involved, also Lavandula hybrida [177].…”

Section: Fungimentioning

confidence: 99%

Entomopathogenic Fungi and Bacteria in a Veterinary Perspective

Ebani

Mancianti

2021

Biology

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The present study aimed to review the papers dealing with the biological activity of fungi and bacteria against some mites and ticks of veterinary interest. In particular, the attention was turned to the research regarding acarid species, Dermanyssus gallinae and Psoroptes sp., which are the cause of severe threat in farm animals and, regarding ticks, also pets. Their impact on animal and human health has been stressed, examining the weaknesses and strengths of conventional treatments. Bacillus thuringiensis, Beauveria bassiana and Metarhizium anisopliae are the most widely employed agents. Their activities have been reviewed, considering the feasibility of an in-field application and the effectiveness of the administration alone or combined with conventional and alternative drugs is reported.

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Effect of Metarhizium anisopliae (Ascomycete), Cypermethrin, and D-Limonene, Alone and Combined, on Larval Mortality of Rhipicephalus sanguineus (Acari: Ixodidae)

Cited by 9 publications

References 22 publications

Ovicidal Efficacy of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) towards Rhipicephalus sanguineus (Acari: Ixodidae) Eggs

Ovicidal Efficacy of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) towards Rhipicephalus sanguineus (Acari: Ixodidae) Eggs

D-Limonene: Promising and Sustainable Natural Bioactive Compound

Entomopathogenic Fungi and Bacteria in a Veterinary Perspective

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