Effect of Mesenchymal Stem Cells on the Vascularization of the Artificial Site for Islet Transplantation in Rats

Fábryová, Eva; Jirák, Daniel; Girman, Peter; Zacharovová, K.; Gálisová, Andrea; Saudek, F; Kříž, Jan

doi:10.1016/j.transproceed.2014.05.074

Cited by 12 publications

(21 citation statements)

References 9 publications

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“…The improvement of blood supply into scaffolds after transplantation of mesenchymal stem cells has already been demonstrated over a 4-week period, but the long-term effect has only been predicted [8]. In the present study, MSCs with luciferase expression were implemented in order to assess the effect of MSCs on scaffold engraftment in a longer period and in more detail using multimodal imaging.…”

Section: Discussionmentioning

confidence: 99%

“…The advantages of subcutaneous implantation are as follows: minimally invasive surgery and the possibility to easily monitor islets. However, the subcutaneous vascular network is inadequate and needs to be enhanced for successful engraftment [8–10]. Pancreatic islets are sensitive to hypoxia, which is even emphasised in environments with high concentrations of glucose typical of a diabetic recipient.…”

Section: Introductionmentioning

confidence: 99%

“…In our group’s previous work, the experiments with dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) showed that blood supply benefits from MSC transplantation [8]. However, the long-term effect of MSCs has not been examined yet.…”

Section: Introductionmentioning

confidence: 99%

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Multimodal Imaging Reveals Improvement of Blood Supply to an Artificial Cell Transplant Site Induced by Bioluminescent Mesenchymal Stem Cells

et al. 2016

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PurposeAn artificial site for cell or pancreatic islet transplantation can be created using a polymeric scaffold, even though it suffers subcutaneously from improper vascularisation. A sufficient blood supply is crucial for graft survival and function and can be enhanced by transplantation of mesenchymal stem cells (MSCs). The purpose of this study was to assess the effect of syngeneic MSCs on neoangiogenesis and cell engraftment in an artificial site by multimodal imaging.ProceduresMSCs expressing a gene for luciferase were injected into the artificial subcutaneous site 7 days after scaffold implantation. MRI experiments (anatomical and dynamic contrast-enhanced images) were performed on a 4.7-T scanner using gradient echo sequences. Bioluminescent images were acquired on an IVIS Lumina optical imager. Longitudinal examination was performed for 2 months, and one animal was monitored for 16 months.ResultsWe confirmed the long-term presence (lasting more than 16 months) of viable donor cells inside the scaffolds using bioluminescence imaging with an optical signal peak appearing on day 3 after MSC implantation. When compared to controls, the tissue perfusion and vessel permeability in the scaffolds were significantly improved at the site with MSCs with a maximal peak on day 9 after MSC transplantation.ConclusionsOur data suggest that the maximal signal obtained by bioluminescence and magnetic resonance imaging from an artificially created site between 3 and 9 days after MSC transplantation can predict the optimal time range for subsequent cellular or tissue transplantation, including pancreatic islets.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multimodal Imaging Reveals Improvement of Blood Supply to an Artificial Cell Transplant Site Induced by Bioluminescent Mesenchymal Stem Cells

et al. 2016

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“…Nevertheless, it should be acknowledged that MRI does not detect the transplanted cells themselves, but the magnetic label they carry. Therefore, MRI cannot respond to cell viability . We presume that the nanoparticles remained in the poorly vascularized muscle tissue (probably inside macrophages) even in the case of cell death.…”

Section: Discussionmentioning

confidence: 97%

“…AT‐MSCs were isolated from visceral adipose tissue according to a standard protocol . Briefly, the tissue was digested by collagenase, filtered, over‐layered on 5 mL of Ficoll 1.077 g/mL (Ficoll‐Paque Premium, GE Healthcare Bio Sciences AB, Uppsala, Sweden) and spun.…”

Section: Methodsmentioning

confidence: 99%

Manganese‐Zinc Ferrites: Safe and Efficient Nanolabels for Cell Imaging and Tracking In Vivo

et al. 2019

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Manganese‐zinc ferrite nanoparticles were synthesized by using a hydrothermal treatment, coated with silica, and then tested as efficient cellular labels for cell tracking, using magnetic resonance imaging (MRI) in vivo. A toxicity study was performed on rat mesenchymal stem cells and C6 glioblastoma cells. Adverse effects on viability and cell proliferation were observed at the highest concentration (0.55 mM) only; cell viability was not compromised at lower concentrations. Nanoparticle internalization was confirmed by transmission electron microscopy. The particles were found in membranous vesicles inside the cytoplasm. Although the metal content (0.42 pg Fe/cell) was lower compared to commercially available iron oxide nanoparticles, labeled cells reached a comparable relaxation rate R 2, owing to higher nanoparticle relaxivity. Cells from transgenic luciferase‐positive rats were used for in vivo experiments. Labeled cells were transplanted into the muscles of non‐bioluminescent rats and visualized by MRI. The cells produced a distinct hypointense signal in T2‐ or T2*‐weighted MR images in vivo. Cell viability in vivo was verified by bioluminescence.

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A Trimodal Imaging Platform for Tracking Viable Transplanted Pancreatic Islets In Vivo: F-19 MR, Fluorescence, and Bioluminescence Imaging

et al. 2018

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We developed a trimodal imaging platform for in vivo examination of transplanted PIs. Fluorescence imaging revealed instability of the fluorescent dye and its limited applicability for longitudinal in vivo studies. A correlation between the bioluminescence signal and the F-19 MRI signal indicated the fast clearance of PLGA-NPs from the transplantation site after cell death, which addresses a major issue with intracellular imaging labels. Therefore, the proposed PLGA-NP platform is reliable for reflecting the status of transplanted PIs in vivo.

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Effect of Mesenchymal Stem Cells on the Vascularization of the Artificial Site for Islet Transplantation in Rats

Cited by 12 publications

References 9 publications

Multimodal Imaging Reveals Improvement of Blood Supply to an Artificial Cell Transplant Site Induced by Bioluminescent Mesenchymal Stem Cells

Multimodal Imaging Reveals Improvement of Blood Supply to an Artificial Cell Transplant Site Induced by Bioluminescent Mesenchymal Stem Cells

Manganese‐Zinc Ferrites: Safe and Efficient Nanolabels for Cell Imaging and Tracking In Vivo

A Trimodal Imaging Platform for Tracking Viable Transplanted Pancreatic Islets In Vivo: F-19 MR, Fluorescence, and Bioluminescence Imaging

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