Effect of MES [2(N‐morpholino)ethanesulfonic acid] and amberlite IRC‐50 resin on nutrient pH control and soybean growth

Nicholas, Joseph C.; Harper, James E.

doi:10.1080/01904169309364582

Cited by 10 publications

(8 citation statements)

References 8 publications

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“…It was also found that, buffering effect of MES is strongly in uenced by its concentration. Nicholas and Harper (1993) reported that MES has a buffering role only in a period of 5 days after adding to the culture, while in the present study, the rst pH recording was happened after 7 days from the culturing time, means we likely lost the opportunity to record the buffering role of MES. Interestingly, as a new nding in this study, it should be stated that, MES had an increasing effect on the cell biomass and crocin content.…”

Section: -Discussioncontrasting

confidence: 53%

Optimization of conditions for increasing of saffron cell biomass and crocin production in stirred bioreactor

Amini

Ziaratnia²,

Hemmati

2022

Plant Cell Tiss Organ Cult

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Bioreactors provide suitable conditions for the growth of cells and production of secondary metabolites by regulating physical and chemical factors. In this study, rst, sucrose, 2-(N-morpholino) ethanesulfonic acid (MES) as a buffering agent and medium pH was optimized in the Erlenmeyer ask. This aim was then pursued in a stirred bioreactor through aeration and pH medium adjustment. Results of the rst step showed that Schenk and Hildebrandt (SH) basal medium with naphthalene acetic acid (2 mg.l -1 ) and 6benzylaminopurine (1 mg.l -1 ) supplemented with 2.5 mM of MES and gradually increment of sucrose from 3 to 6% caused to catch the highest cell biomass and crocin production. The spectrophotometry measurement showed that the highest crocin content of the cells was 0.8 mg/g after ve weeks. The results of the second part revealed that in the stirred bioreactor, constant pH (5.8) during the growth period is a limited factor for the cell growth and crocin production. Although aeration initially found to be an inhibited factor for the production of crocin, results showed that, if the evaporated volume of water caused by aeration is constituted, it can be an effective factor to increase cell growth rate around 2 folds.In addition, total crocin content of the cells, based on the HPLC could be raised up to 2 mg/g. Based on this study, it can be concluded that MES and gradual increment of sucrose could increasing the cell growth and crocin production. Aeration in bioreactor can increase cell biomass, if the medium volume will be kept constant. Key MessageSaffron, the most expensive spice contains valuable compounds like crocin. Corm derived-cell containing crocin can be produced in higher scales and cheaper price by using cell culture in stirred bioreactor.

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Section: -Discussioncontrasting

confidence: 53%

Optimization of conditions for increasing of saffron cell biomass and crocin production in stirred bioreactor

Amini

Ziaratnia²,

Hemmati

2022

Plant Cell Tiss Organ Cult

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“…The pH was gradually decreased from 6.5 to 4.0. Potential problems with MES were pointed out previous study ( Nicholas and James, 1993 ). All this suggests that the long-term experiments (longer than a day), that require fine pH control in liquid or solidified media, must be interpreted with a great care.…”

Section: Discussionmentioning

confidence: 89%

“…Importantly, 4 mM MES cannot maintain pH value for long period in hydroponic condition. The pH was decreased from 6.5 to 4.0 just for 5 days ( Nicholas and James, 1993 ). It was also reported that MES inhibits the adventitious root formation from apple stem disks at 10 mM of concentration ( De Klerk et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

MES Buffer Affects Arabidopsis Root Apex Zonation and Root Growth by Suppressing Superoxide Generation in Root Apex

2016

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In plants, growth of roots and root hairs is regulated by the fine cellular control of pH and reactive oxygen species (ROS). MES, 2-(N-morpholino)ethanesulfonic acid as one of the Good’s buffers has broadly been used for buffering medium, and it is thought to suit for plant growth with the concentration at 0.1% (w/v) because the buffer capacity of MES ranging pH 5.5–7.0 (for Arabidopsis, pH 5.8). However, many reports have shown that, in nature, roots require different pH values on the surface of specific root apex zones, namely meristem, transition zone, and elongation zone. Despite the fact that roots always grow on a media containing buffer molecule, little is known about impact of MES on root growth. Here, we have checked the effects of different concentrations of MES buffer using growing roots of Arabidopsis thaliana. Our results show that 1% of MES significantly inhibited root growth, the number of root hairs and length of meristem, whereas 0.1% promoted root growth and root apex area (region spanning from the root tip up to the transition zone). Furthermore, superoxide generation in root apex disappeared at 1% of MES. These results suggest that MES disturbs normal root morphogenesis by changing the ROS homeostasis in root apex.

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“…Ion exchange resins have previously been investigated as a means of controlling the pH of nutrient solutions (Hageman et al, 1961;Harper and Nicholas, 1976;Nicholas and Harper, 1993). However, resins (typically weak cation exchangers) have only been used to buffer solutions in the neutral to acidic range.…”

Section: Experiments 3 -Ion Exchange Resins As Buffersmentioning

confidence: 99%

Control of nutrient solutions for studies at high pH

Kopittke

Menzies

2005

Plant Soil

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Little is known about factors effecting plant growth at high pH, with research often limited by the inability to separate nutritional deficiencies and HCO − 3 toxicity from the direct limitations imposed under high pH conditions. Various methods of controlling dilute nutrient solutions for studies at high pH were investigated. For short-term studies, it was found that a solution without Cu, Fe, Mn and Zn and aerated with CO 2 depleted air, greatly reduced nutrient precipitation at high pH, thus eliminating nutritional differences between treatments. Manual pH adjustment and the use of ion exchange resins as pH buffers were unsuitable methods of pH control. However, pH control by automated titration had little effect on solution composition while maintaining constant pH. The system described is suitable for studies in which the pH of the bulk nutrient solution must be maintained. The system was used to examine OH − toxicity in mungbeans (Vigna radiata (L.) Wilczek cv. Emerald), with root length reduced at a bulk solution pH of 8.5 and greater.Abbreviations: DI -deionised; EC -electrical conductivity; ICPAES-inductively coupled plasma atomic emission spectroscopy; LSD -least significant difference.

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Effect of MES [2(N‐morpholino)ethanesulfonic acid] and amberlite IRC‐50 resin on nutrient pH control and soybean growth

Cited by 10 publications

References 8 publications

Optimization of conditions for increasing of saffron cell biomass and crocin production in stirred bioreactor

Optimization of conditions for increasing of saffron cell biomass and crocin production in stirred bioreactor

MES Buffer Affects Arabidopsis Root Apex Zonation and Root Growth by Suppressing Superoxide Generation in Root Apex

Control of nutrient solutions for studies at high pH

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