Effect of Membrane-bound Aldehyde Dehydrogenase-encoding Gene Disruption on Glyceric Acid Production in Gluconobacter oxydans

Habe, Hiroshi; Sato, Shun; Fukuoka, Tokuma; Kitamoto, Daï; Sakaki, Keiji

doi:10.5650/jos.ess14112

Cited by 2 publications

(3 citation statements)

References 13 publications

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“…The genetic modification of G. oxydans 12528, consisting of the cutting out of the adhA gene, caused the halt of acetic acid production from ethyl alcohol and lowered by half the production of glyceric acid, which was still being produced despite the absence of mADH. Study results suggest that another enzyme could be present in the mutant's cells, that could take part in the production of glyceric acid [14].…”

Section: Application Of Genetically-modified Strains Of Aab In the Inmentioning

confidence: 93%

“…Habe et al [13,15] facilitated the mutation of AAB from the species Gluconobacter frateurii. The wild strain was carrying out biotransformation of glycerol to glyceric acid, while glycerol dehydrogenase (GlyDH, EC 1.1.99.22) was catalyzing the oxidation of substrate to DHA, which was accumulated in bacterial cells [13][14][15]. In order to facilitate the recovery of pure gluconic acid from the post-reaction mixture, the sldA gene encoding GlyDH was removed from the genome of G. frateurii.…”

Section: Application Of Genetically-modified Strains Of Aab In the Industrymentioning

confidence: 99%

“…An example of the undesirable (from the perspective of the food industry) effect of genetic modifications on the production of selected chemical compounds is also the mutation of G. oxydans IFO 12528 [14]. This strain is capable of producing glyceric acid from glycerol in the presence of alcoholic dehydrogenase (mADH), which is, in turn, encoded by the adhA gene.…”

Section: Application Of Genetically-modified Strains Of Aab In the Industrymentioning

confidence: 99%

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Industrial Applications of Wild and Genetically-Modified Strains of Acetic Acid Bacteria

Stasiak-Różańska

Kupiec

2018

Postępy Mikrobiologii - Advancements of Microbiology

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Acetic acid bacteria (AAB) are Gram-negative, non-spore-forming, aerobic bacilli [40]. The optimal temperature for their growth ranges from 25 to 35°C, and optimal pH-from 5.4 to 6.3 [11, 28]. AAB are chemoorganotrophs [26]. They may develop in an environment with high osmotic pressure, e.g. in fruits and fruit juices, nectars, bee honeys, ciders or beers [8, 29]. A typical trait of all AAB is its capability of producing acids that are formed as the terminal or transient products of oxidation of alcohols and carbohydrates. The key compounds produced by AAB include: acetic acid, bacterial cellulose, dihydroxyacetone, gluconic acid and levan [30, 33, 35, 38]. These bacteria are also promising starter cultures, used either to better control known food fermentation processes or to produce novel fermented foods and beverages. They play an important role in natural food fermentation processes such as lambic beer, water kefir, kombucha, and cocoa [6]. The most popular product obtained from AAC is vinegar. This compound is mainly used for food preservation, but also for flavour enhancement of dishes. Vinegar is additionally used for the preparation of sauces, mayonnaises and mustards. It improves the sensory attributes of a ready product, and, through its acidifying medium, enables the preservation of food [29]. Recently, there has been a big increase in interest and research regarding the obtaining of bacterial cellulose (BC, also known as microbial cellulose). BC exists as a basic structure of fibril that consist of a β-1,4glucan chain with the molecular formula (C 6 H 10 O 5)n. The glucan chains are held together by inter-and intra-hydrogen bonding. Microfibryls of BC are about 100 times smaller than plant cellulose [9]. Moreover, the three dimensional structure of BC is much more

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Section: Application Of Genetically-modified Strains Of Aab In the Inmentioning

confidence: 93%

Section: Application Of Genetically-modified Strains Of Aab In the Industrymentioning

confidence: 99%

Section: Application Of Genetically-modified Strains Of Aab In the Industrymentioning

confidence: 99%

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Industrial Applications of Wild and Genetically-Modified Strains of Acetic Acid Bacteria

Stasiak-Różańska

Kupiec

2018

Postępy Mikrobiologii - Advancements of Microbiology

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Engineering of glycerol utilization in Gluconobacter oxydans 621H for biocatalyst preparation in a low-cost way

Yan

Cao

et al. 2018

Microb Cell Fact

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BackgroundWhole cells of Gluconobacter oxydans are widely used in various biocatalytic processes. Sorbitol at high concentrations is commonly used in complex media to prepare biocatalysts. Exploiting an alternative process for preparation of biocatalysts with low cost substrates is of importance for industrial applications.Results G. oxydans 621H was confirmed to have the ability to grow in mineral salts medium with glycerol, an inevitable waste generated from industry of biofuels, as the sole carbon source. Based on the glycerol utilization mechanism elucidated in this study, the major polyol dehydrogenase (GOX0854) and the membrane-bound alcohol dehydrogenase (GOX1068) can competitively utilize glycerol but play no obvious roles in the biocatalyst preparation. Thus, the genes related to these two enzymes were deleted. Whole cells of G. oxydans ∆GOX1068∆GOX0854 can be prepared from glycerol with a 2.4-fold higher biomass yield than that of G. oxydans 621H. Using whole cells of G. oxydans ∆GOX1068∆GOX0854 as the biocatalyst, 61.6 g L−1 xylonate was produced from 58.4 g L−1 xylose at a yield of 1.05 g g−1.ConclusionThis process is an example of efficient preparation of whole cells of G. oxydans with reduced cost. Besides xylonate production from xylose, other biocatalytic processes might also be developed using whole cells of metabolic engineered G. oxydans prepared from glycerol.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-1001-0) contains supplementary material, which is available to authorized users.

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Effect of Membrane-bound Aldehyde Dehydrogenase-encoding Gene Disruption on Glyceric Acid Production in Gluconobacter oxydans

Cited by 2 publications

References 13 publications

Industrial Applications of Wild and Genetically-Modified Strains of Acetic Acid Bacteria

Industrial Applications of Wild and Genetically-Modified Strains of Acetic Acid Bacteria

Engineering of glycerol utilization in Gluconobacter oxydans 621H for biocatalyst preparation in a low-cost way

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