Effect of Melatonin on Testicular Damage in Streptozotocin-Induced Diabetes Rats

Abstract

Background: It is well known that diabetes mellitus is associated with impairment of testicular function. In the present study, we aimed to demonstrate the effect of melatonin on testicular damage in male rats with streptozotocin (STZ)-induced diabetes. Methods: Male Wistar rats were divided into 4 groups: (1) control group, (2) melatonin-treated nondiabetic group, (3) diabetic group and (4) melatonin-treated diabetic group. Diabetes was induced by STZ injection. Melatonin was administered intraperitoneally at the dose of 10 mg/kg for 5 days. Testicular damage was examined by using hematoxylin and eosin staining and periodic acid-Schiff staining, and apoptosis was determined by terminal-deoxynucleotidyl-transferase-mediated dUTP nick end labeling (TUNEL). Potential disorders associated with seminiferous tubular sperm formation were evaluated using the Johnsen score. Results: Diabetic rats showed a reduction in seminiferous tubule diameter, increased thickening of the basement membrane in seminiferous tubules and degenerated germ cells. TUNEL-positive cells were significantly more numerous in diabetic rats than in control rats. Melatonin significantly attenuated the diabetes-induced morphological changes and germ cell apoptosis in the diabetic rat testis. The number of polymorphonuclear leukocytes was significantly decreased in group 4 when compared to group 3. Conclusions: These results suggest that intraperitoneal administration of melatonin for 5 days is a potentially beneficial agent to reduce testicular damage in adult diabetic rats, probably by decreasing oxidative stress.

Effect of Photoperiod and Melatonin on Volume and Cellular Parameters of Testis in Pinealectomized Rats

“…However, melatonin administration in late afternoon had decreased diameter of seminiferous tubule in non-PE hamster and mice (Ng & Ooi; Ooi & Ng). Nonetheless, if the antioxidant activity considered of the melatonin, we noted that melatonin administration increased the diameter of the seminiferous tubules in the diabetic rats (Guneli et al, 2008), healed the harmful effects of the radiation in the testis, restarted the spermatogenesis (Hussein et al, 2006) and protected the epithelial height of the seminiferous tubule in poisoned animals by pesticides (Sarabia et al). In addition to these findings, it was found that the exogenous melatonin administration protect testis towards the oxidative stressinduced tissue damage emerges after pinealectomy (Tasdemir et al, 2012).…”

Melatonin Ratlarda Alloxan İle İndüklenen Diyabet Sonrası Oluşan Testiküler Hasarı ve Oksidatif Etkileri Hafifletir

“…The Melatonin Attenuates ... parameters in the DM group rats [10][11][12][13][14] . The protective effects of MLT (used herein as an antioxidant) on the testis tissue are consistent with the previous results in DM models [11,12,44] .…”

“…The MLT is a freeradical scavenger and wide-spectrum antioxidant, and it stimulates the gene expression of antioxidant and prooxidant enzymes. MLT treatment after alloxan-induced b-cell destruction has been reported recently [10][11][12][13][14] . These studies suggest that MLT treatment in DM regulates the antioxidant enzymes and decreases the degenerative changes.…”

An Experimental Model Study: Histomorphological and Ultrastructural Renal Changes in 1/2-5/6 Nephrectomized and Diabetic Rats

“…EXPERIMENTAL MODELS D Di ia ab be et te es s m me el ll li it tu us s ( (D DM M) ) m mo od de el l: : DM was induced by administration of intraperitoneal STZ (S0130-1G, Sigma, Aldrich) (dissolved in citrate buffer of pH 4.5) at a dose of 45 mg/kg to the rats. 17 On the 3rd day after STZ administration, nonfasting serum glucose levels were measured using a Medisense Optium glucometer (Roche Diagnostic, Germany). Rats with blood glucose levels higher than 250 mg/dl were considered as diabetic.…”