Effect of Melatonin on Expression of Apoptosis Regulator Proteins Bcl-2 and Bad in Ovarian Follicular Apparatus after High Temperature Exposure

Мичурина, С. В.; Колесников, С. И.; Ishchenko, I. Yu.; Bochkareva, Aleksandra; Архипов, С. А.

doi:10.1007/s10517-021-05114-6

Cited by 3 publications

(3 citation statements)

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“…AKT can regulate Bad activity by phosphorylating at Ser-136 [ 179 ]. Dephosphorylated Bad was able to form a pro-apoptotic complex with anti-apoptotic factors Bcl-2 or Bcl-xL, which activated pro-apoptotic members such as Bax or Bak, and jointly promoted cell apoptosis [ 180 ]. Studies have shown that H 2 O 2 can inhibit the PI3K/AKT pathway, and induce the elevated expression of Bad, Bax and CASP9, further accelerating granulosa cell apoptosis [ 181 ].…”

Section: Os-related Signaling Pathways In Ovarian Agingmentioning

confidence: 99%

The role of oxidative stress in ovarian aging: a review

et al. 2022

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Ovarian aging refers to the process by which ovarian function declines until eventual failure. The pathogenesis of ovarian aging is complex and diverse; oxidative stress (OS) is considered to be a key factor. This review focuses on the fact that OS status accelerates the ovarian aging process by promoting apoptosis, inflammation, mitochondrial damage, telomere shortening and biomacromolecular damage. Current evidence suggests that aging, smoking, high-sugar diets, pressure, superovulation, chemotherapeutic agents and industrial pollutants can be factors that accelerate ovarian aging by exacerbating OS status. In addition, we review the role of nuclear factor E2-related factor 2 (Nrf2), Sirtuin (Sirt), mitogen-activated protein kinase (MAPK), protein kinase B (AKT), Forkhead box O (FoxO) and Klotho signaling pathways during the process of ovarian aging. We also explore the role of antioxidant therapies such as melatonin, vitamins, stem cell therapies, antioxidant monomers and Traditional Chinese Medicine (TCM), and investigate the roles of these supplements with respect to the reduction of OS and the improvement of ovarian function. This review provides a rationale for antioxidant therapy to improve ovarian aging.

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Section: Os-related Signaling Pathways In Ovarian Agingmentioning

confidence: 99%

The role of oxidative stress in ovarian aging: a review

et al. 2022

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“…В результате настоящего исследования мы установили, что введение МТ крысам после гипертермии сдвигает соотношение площадей экспрессии Bcl-2/Bad в жёлтых телах яичников в сторону увеличения антиапоптотического Bcl-2 уже на 7-е сутки восстановительного периода, в отличие от животных, не получавших лечения этим гормоном, что способствует нормализации Bcl-2/ Bad до физиологического уровня (уже на 14-е сутки). В более раннем исследовании нами также было выявлено, что МТ может ингибировать митохондриальный путь апоптоза в фолликулах яичников крыс уже на 7-й день постгипертермии [5].…”

Section: Microphotographs Of Rat Ovarian Corpus Luteum On Day 14 Afte...unclassified

“…Считается, что МТ даже может синтезироваться в митохондриях [4]. Одним из наиболее значимых физиологических свойств эпифизарного МТ является его способность регулировать клеточную пролиферацию и апоптоз [5,6].…”

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The effect of melatonin on the Bcl-2 and Bad proteins expression in ovarian corpus luteum cells after exposure to experimental hyperthermia

Michurina,

Kolesnikov,

Ishchenko

et al. 2024

Acta biomedica scientifica

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Background. There is growing interest in determining the role of melatonin in the regulation of proliferation and apoptosis of ovarian cells at various diseases and destabilizing influences. It is believed that the choice between the implementation of a cell death or survival program determines the ratio of anti-apoptotic and pro-apoptotic proteins.The aim. To identify the effect ofmelatonin onthe expression ofanti-apoptotic Bcl-2 and pro-apoptotic Bad and the Bcl-2/Bad ratio in the ovarian luteocytes of Wistar rats in the acute (day 3) and recovery (days 7 and 14) periods after a single exposure to experimental hyperthermia.Methods. Warming up took no more than 17 minutes. Melatonin was injected subcutaneously (0.1 mg in 0.2 ml of physiological solution) for 3 days after experimental hyperthermia. Comparison groups included rats with physiological solution injection (control) and animals after experimental hyperthermia + physiological solution injection. The Bad and Bcl-2 expression was determined immunohistochemically on days 3, 7 and 14 after experimental hyperthermia + physiological solution or melatonin injection.Results. On the day 3 after experimental hyperthermia, the effect of the hormone was not detected. A week after experimental hyperthermia + melatonin injection, the Bad expression area decreased more significantly than in rats after experimental hyperthermia + physiological solution injection, which led to an increase in Bcl-2/ Bad ratio. This indicated an increase in anti-apoptotic protection, blocking the development of the internal apoptosis pathway at this time. 2 weeks after experimental hyperthermia + physiological solution injection, the Bcl-2 area decreased more significantly than the Bad area. As a result, the Bcl-2/Bad ratio decreased almost 2-fold compared to the control group. This indicated the activation of the “mitochondrial branch” of luteocyte apoptosis. 2 weeks after experimental hyperthermia + melatonin injection, the Bad and Bcl-2 areas decreased synchronously, which restored Bcl-2/ Bad to control values.Conclusion. The melatonin injection after experimental hyperthermia shifts the ratio of Bcl-2/Bad expression areas towards an increase in anti-apoptotic Bcl2 already a week after the recovery period and promotes earlier normalization of Bcl-2/Bad to physiological levels (as early as 2 weeks after experimental hyperthermia + melatonin injection).

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Experimental hyperthermia: expression of proteins involved in the regulation of ovarian corpus luteum apoptosis in the acute and recovery periods

Мичурина

Kolesnikov

Ishchenko

et al. 2022

Acta biomedica scientifica

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Background.Heat shock effects can initiate apoptosis of oocytes and corpus luteum cells in mammalian ovaries. During folliculogenesis, follicular apoptosis is regulated by Bcl-2 and BAX proteins which are key effectors of granular cell death. Mechanisms of disruption of the ovarian corpus luteum development under heat stress remain largely unclear.Aim of the research: to identify the expression features of anti-apoptotic Bad and proapoptotic Bcl-2 proteins in the rat ovarian luteocytes in the acute (by day 3) and recovery (by days 7 and 14) periods after a single exposure of experimental hyperthermia (EH) (rectal temperature 43.5 °C).Materials and methods. The expression of Bad and Bcl-2 was determined immunohistochemically using an indirect two-stage streptavidin-biotin method.Results. On day 3 after EH, the expression areas of both Bad and Bcl-2 increased 2-fold, but the ratio of Bcl-2/Bad areas did not change, indicating that the intensity of apoptosis along the mitochondrial pathway in luteocytes in the acute period was maintained within physiological values. On day 7, the Bad and Bcl-2 expression areas remained at the level of day 3, but the Bcl-2/Bad index decreased, indicating the activation of the apoptosis internal pathway in the ovarian corpus luteum cells. By day 14, the protein expression areas decreased (Bad – by 1.7 times, Bcl-2 – by 3.2 times) compared to the acute period, and the Bcl-2/Bad index decreased by 2 times compared to the control and the acute period group.Conclusion. The observed predominance of proapoptotic Bad protein over antiapoptotic Bcl-2 in luteocytes on day 14 after EH indicates the anti-apoptotic protection violation, which leads to the apoptosis mitochondrial pathway activation of the latter. A decrease in Bcl-2 expression can be regarded as a manifestation of the defective luteocytes removal mechanism and the body’s desire to normalize the ovarian-uterine cycle disrupted by high temperature exposure.

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Effect of Melatonin on Expression of Apoptosis Regulator Proteins Bcl-2 and Bad in Ovarian Follicular Apparatus after High Temperature Exposure

Cited by 3 publications

References 13 publications

The role of oxidative stress in ovarian aging: a review

The role of oxidative stress in ovarian aging: a review

The effect of melatonin on the Bcl-2 and Bad proteins expression in ovarian corpus luteum cells after exposure to experimental hyperthermia

Experimental hyperthermia: expression of proteins involved in the regulation of ovarian corpus luteum apoptosis in the acute and recovery periods

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