Effect of melatonin on bovine theca cells in vitro

Feng, Tao; Schütz, Luís F.; Morrell, B.C.; Perego, Maria Chiara; Spicer, L. J.

doi:10.1071/rd17203

Cited by 9 publications

(2 citation statements)

References 66 publications

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“…In agreement with a previous study of goat TCs, coculture with GCs did not significantly increase the expression of steroidogenic genes in TCs 20. In a previous study on bovine TCs from large follicles (8.0-22.0 mm), melatonin inhibited the LH+IGF1-induced expression of STAR mRNA 48. A possible explanation for this might be that the melatonin concentration used (10 mM = 2.3 μg/mL) was much higher than that used in the present study (10 ng/mL).…”

Section: Discussionsupporting

confidence: 92%

Melatonin Stimulates STAR Expression and Progesterone Production via Activation of the PI3K/AKT Pathway in Bovine Theca Cells

Wang

Meng

et al. 2019

Int. J. Biol. Sci.

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Melatonin is present in mammalian follicular fluid and plays an important role in regulating steroidogenesis in follicular cells. In this study, we report the effect of melatonin on steroidogenesis in the theca interna (TI) in small bovine follicles and theca cells (TCs) cultured in vitro. Treatment with melatonin significantly increased the expression of steroidogenic acute regulatory protein (STAR) and the production of progesterone in both TI and in TCs. Melatonin stimulated the phosphorylation of AKT but not ERK1/2, and the addition of luzindole (a nonspecific MT1 and MT2 inhibitor) or 4P-PDOT (specific MT2 inhibitor) reduced melatonin-induced STAR expression, progesterone secretion, and PI3K/AKT pathway activation. The effect of melatonin on the TI in follicles was more obvious than on the TCs in vitro. Results indicate that melatonin stimulates the steroidogenesis of TCs mainly via the activation of the PI3K/AKT pathway by MT1 and MT2.

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Section: Discussionsupporting

confidence: 92%

Melatonin Stimulates STAR Expression and Progesterone Production via Activation of the PI3K/AKT Pathway in Bovine Theca Cells

Wang

Meng

et al. 2019

Int. J. Biol. Sci.

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“…Melatonin inhibits LH + insulin-like growth factor 1 (IGF1)-induced androstenedione and progesterone production as well as the expression of steroidogenic acute regulatory protein (StAR) mRNA (via real-time polymerase chain reaction) in Theca cells. Melatonin has no effect on cytochrome P450 11A1 (CYP11A1) and cytochrome P450 17A1 (CYP17A1) mRNA abundance [ 128 ]. Melatonin increases apoptosis, induced by cisplatin, by inhibiting the JNK/Parkin/mitophagy axis [ 129 ].…”

Section: Melatonin Has Many Effects and Targetsmentioning

confidence: 99%

Melatonin: Facts, Extrapolations and Clinical Trials

2023

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Melatonin is a fascinating molecule that has captured the imagination of many scientists since its discovery in 1958. In recent times, the focus has changed from investigating its natural role as a transducer of biological time for physiological systems to hypothesized roles in virtually all clinical conditions. This goes along with the appearance of extensive literature claiming the (generally) positive benefits of high doses of melatonin in animal models and various clinical situations that would not be receptor-mediated. Based on the assumption that melatonin is safe, high doses have been administered to patients, including the elderly and children, in clinical trials. In this review, we critically review the corresponding literature, including the hypotheses that melatonin acts as a scavenger molecule, in particular in mitochondria, by trying not only to contextualize these interests but also by attempting to separate the wheat from the chaff (or the wishful thinking from the facts). We conclude that most claims remain hypotheses and that the experimental evidence used to promote them is limited and sometimes flawed. Our review will hopefully encourage clinical researchers to reflect on what melatonin can and cannot do and help move the field forward on a solid basis.

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