Effect of melatonin on actin and α-tubulin distribution related to in vitro capacitation in ram sperm

Carvajal‐Serna, Melissa; Paesa, Mónica; Barale, Juliano; Gimeno-Martos, Silvia; Peña-Delgado, Victoria; Miguel-Jiménez, Sara; Casao, A.; Pérez‐Pé, Rosaura

doi:10.1016/j.anireprosci.2020.106363

Cited by 1 publication

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“…Moreover, we have shown here that melatonin was able to modify the NOX5 distribution in ram spermatozoa, preventing the changes in localization provoked by in vitro capacitation. It is worth mentioning that we have recently reported the effect of melatonin on cytoskeletal remodeling during in vitro ram sperm capacitation, concluding that 1 µM melatonin prevents such changes, according to the actin and α-tubulin distribution (Carvajal-Serna et al, 2020). The melatonin action on the cytoskeleton has been also reported in somatic cells (Benítez-King, 2006).…”

Section: Discussionmentioning

confidence: 81%

NADPH Oxidase 5 and Melatonin: Involvement in Ram Sperm Capacitation

Miguel-Jiménez

Pina-Beltrán

Gimeno-Martos

et al. 2021

Front. Cell Dev. Biol.

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Reactive oxygen species (ROS) play an essential role in mammalian sperm capacitation. NADPH oxidase 5 (NOX5) has been described as the main source of ROS production in some mammalian spermatozoa, such as human and equine. On the other hand, melatonin can decrease cellular ROS levels and regulates NOX activity in somatic cells. Therefore, the objectives of this work were (1) to identify NOX5 in ram spermatozoa and analyze its possible changes during in vitro capacitation and (2) to investigate the effect of melatonin on NOX5 expression and localization and on superoxide levels in capacitated ram spermatozoa. Protein bands associated with NOX5 were detected by Western blot analysis. Likewise, indirect immunofluorescence (IIF) revealed six different immunotypes for NOX5, which varied throughout in vitro capacitation. Superoxide (O2⋅–), evaluated by DHE/Yo-Pro-1, rose after in vitro capacitation and in the presence of the calcium ionophore A23187 but decreased in the presence of the NOX inhibitor GKT136901. GKT also reduced the percentage of capacitated and acrosome-reacted spermatozoa that had increased during incubation in capacitating conditions. The presence of melatonin at micromolar concentrations avoided the increment in O2⋅– and the changes in NOX5 immunotypes provoked by capacitation. In conclusion, NOX5 is present in ram spermatozoa and the changes in its distribution, associated with sperm capacitation, can be prevented by melatonin. To this extent, it could imply that melatonin exerts its antioxidant role, at least in part, by modulating NOX5 activity during ram sperm capacitation.

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Section: Discussionmentioning

confidence: 81%