Effect of medium supplementation on exopolysaccharide production by Lactobacillus rhamnosus RW-9595M in whey permeate

Production of Exopolysaccharide by Lactobacillus plantarum and the Prebiotic Activity of the Exopolysaccharide

“…Quantities of EPSs were 50-60 mg/L for Lactobacillus casei, 60-150 mg/L for Lactobacillus bulgaricus (Cerning, 1995), and one of the highest yields was 2775 mg/L from whey permeate supplemented with yeast extract, vitamins, salts and amino acids for Lactobacillus rhamnosus RW-9595M (Macedo et al, 2002). The yield of the EPS for strain 301102S was 145 mg/L in whey supplemented with yeast extract and glucose.…”

The growth and recovery of an exopolysaccharide-producing Lactobacillus rhamnosus culture on growth media containing apple juice or molasses

“…Indeed, statistical analysis showed that there was a significant (p=0.03; R=0.70) relationship between OD readings from the fermentations and capillary relative viscosity (CRV; comparative flow rate, in a capillary, of the fermented medium to that of water). Previous data with this strain also showed that medium composition had an effect of EPS synthesis (Macedo et al, 2002).The relationship between the CRV and the residual OD after centrifugation at 10,000 g was judged to be significant (p=0.02) but the correlation coefficient was not high (R=0.74). This suggests that viscosity is not the only factor which influences the ease by which cells are recovered by centrifugation.…”

“…This suggests that correcting CFU values may better represent population levels, and improve correlations with other methods of ascertaining biomass yields. Recovery of growth media on viscosity: Lactobacillus rhamnosus RW-9595M is a very high exopolysaccharide (EPS) producer (Macedo et al, 2002). The EPS increases the viscosity of the medium and makes it difficult to recover the cells by centrifugation (Champagne et al, 2007).…”

Locticacid bacteria: funcionality, polysaccharides, therapeutic potencial and applications in food

“…Sin embargo, la comparación de estos resultados se difculta debido a las diferentes técnicas de exatracción y cuantifcación de EPS utilizadas por cada autor, sumado al de difícil separación durante el análisis [32](Goh et al 2005). Según [64], la producción de EPS por bacterias lácticas es infuenciada por la composición del medio de crecimiento, particularmente de aminoácidos, minerales, vitaminas y bases nitrogenadas, la cual está relacionada a la producción de biomasa, encontrándose algunas excepciones. Así, se han realizado diversas tentativas para aumentar la producción de EPS, como alteración de la composiçión del medio, como fuentes de carbono.…”

“…Así, se han realizado diversas tentativas para aumentar la producción de EPS, como alteración de la composiçión del medio, como fuentes de carbono. Se ha probado que sales de Mg , por ejemplo, infuye en la actividad de la enzima fosfoglucomutasa, que participa en la biosíntesis de EPS, catalizando la transferencia de grupos fosfato entre los carbono C1 y C6 de la glucosa [64]. Así, como el extracto hidrosoluble de soja, al ser un medio complejo, rico en aminoácidos, vitaminas y sales minerales (~216 mg/L de Mg Se han estudiado y usado por muchos investigadores diferentes técnicas para la determinación de EPS en las últimas dos décadas [24,9,23,22,81,37,65,66,7,20,112,25,34,35].…”