Effect of medium composition and explant size on embryogenic calli formation of cassava (Manihot esculenta Crantz) local genotypes

Abstract

Cassava (Manihot esculenta Crantz) is an important tropical crop species used for human consumption, feed and raw material for various industries. Genetic transformation through embryogenic tissues is known as an effective method for cassava genetic improvements. Objective of this study was to obtain a suitable medium and length of explants to induce embryogenic callus on friable embryogenic callus (FEC) as a target for genetic transformation. Immature leaf lobes (1-3 mm, 3-5 mm and larger than 5 mm in length) of local genotypes of cassava (Adira 4. Menti, Iding, Gebang, Rawi and Timtim-29) cultured in vitro were used as explants. The explants were incubated for 2 and 4 weeks on MS (Murashige-Skoog) or GD (Greshooff & Doy) semi solid medium containing 10 mg/L picloram, 6 mg/L NAA supplemented with 4% sucrose and 4 µM CuSO 4. Results showed that the highest percentage (100%) of embryogenic calli formation for 4 weeks obtained by culturing Iding of 3-5 mm length on GD semi solid medium, whereas the lowest (33%) one obtained by incubation 5 mm leaf lobe of Timtim-29 on the same medium. The most suitable medium for callus induction was GD, whereas the optimum length of explants was 5 mm or larger. Further study needs to be done to obtain friable embryogenic calli (FEC) by employing different concentration of picloram and varying other critical factors.

Embryogenic callus induction of Indonesian Cassava (Menti and Adira 4) on different picloram concentrations

“…Picloram has been reported to induce embryogenic callus in many studies [9,10,11,12], which is in line with this experiment. Zainuddin et al [4] recommended an embryogenic callus induction medium with 12 mg L -1 picloram to produce the embryogenic callus of cassava.…”