Effect of Medicinal Mushrooms on L-arginine/NO System in Red Blood Cells of Streptozotocin-induced Diabetic Rats

Abstract

Increase of nitric oxide production resulted in the development of oxidative-nitrosative stress that is considered to be an etiological cause of many diseases, including diabetes mellitus (DM). Recently, it was found that red blood cells (RBCs) are able to produce nitric oxide (NO), and due to the ability of hemoglobin to bind to nitric oxide, are the main depot of NO. Medicinal mushrooms are widely used in the correction and treatment of many diseases, including diabetes. Our previous results showed that Agaricus brasiliensis and Ganoderma lucidum have hypoglycemic effects and improve the functional state of RBCs. In this study, the influence of submerged cultivated mycelium powder (SCMP) of the abovementioned mushrooms on the red blood cells L-arginine/NO system of streptozotocin-induced DM rats was investigated. Wistar outbread white rats were used in the study. Streptozotocin was intraperitoneal injected one time at a dose of 50 mg/kg body weight. Mushroom preparations were orally administered at a dose of 1 g/kg/day for 14 days. We showed that administration of medicinal mushrooms SCMP to diabetic animals caused restoration of NO-synthase activity, normalized nitrite content (in case of A. brasiliensis), and led to nitrate growth. Therefore, treatment with mushroom mycelia normalizes the production of nitric oxide to physiological values.

Agaricus brasiliensis Mushroom Protects Against Sepsis by Alleviating Oxidative and Inflammatory Response

“…Moreover, the extract maintained the antioxidant defense, preserving the levels of reduced glutathione and protein thiol (52). In this line, it was demonstrated that in diabetic rats, the administration of Ab restored superoxide dismutase, catalase, and gluthatione peroxidase activity (43). This ability may be, at least in part, due to the presence of glutamic acid and glycine in Ab, since these amino acids are precursors of GSH synthesis, where glutamic acid reacts with cysteine to produce γ-glutamylcysteine (GCS) and subsequently reacts with glycine to form GSH (11,53).…”

“…In agreement, it was demonstrated that ( 16)-β-D-glucans activates dectin-1 receptors in monocytes, neutrophils and dendritic cells leading to phosphorylation of Syk and activation of CARD9/Bcl10/MALT-1 4 with consequent augment in phagocytic capacity and increased generation of ROS and RNS (42). Furthermore, according to Vitak et al, Ab is rich in arginine, a precursor to nitric oxide production by NO synthases responsible for the conversion of L-arginine to NO and L-citrulline (43).…”

The corrective effect of chromium and zinc citrates on NO-synthase activity of erythrocytes in rats with streptozotocin diabetes

“…Гіперглікемія, яка розвивається за ЦД, ініціює вироблення мітохондріями активних форм Оксигену (АФО), що призводить до функціонування патологічних метаболічних шляхів: поліолового шляху утилізації глюкози; збільшеного утворення кінцевих продуктів посиленого глікозилювання; гексозамінового патологічного шляху. Негативні наслідки підвищеного рівня глюкози у крові проявляються в інтенсифікації оксидативного стресу та розвитку ендотеліальної дис-функції [11,30]. Причинами, які призводять до розвитку цього патологічного процесу, можуть бути: відсутність синтезу або біодоступності нітроген оксиду (NO) [5] внаслідок зменшення його продукції чи інактивації АФО, які утворюються; глікозилюванням протеїнів; безпосередньо з ендотелію судин [13].…”