Effect of meal and cimetidine on endogenous plasma gastrin, secretin and pancreatic polypeptide in experimental acid hypersecretion in dogs.

Abstract

In order to observe the plasma gastrin, secretin and pancreatic polypeptide (PP) levels in an acid hypersecretion state, the pyloric antrum was transposed onto the transverse colon in 4 mongrel dogs with a Heidenhain pouch. The effects of meal and cimetidine on these gut hormones and acid secretion levels were observed for 6 hr after meals. After antrocolic transposition (ACT), hypersecretion of acid and high plasma gastrin levels were observed both in the fasting and stimulated states. Plasma secretin levels were elevated for 6 postprandial hr, but were reduced by cimetidine as a result of marked suppression of acid secretion. Plasma PP levels also increased for 6 postprandial hr, but its responses were not altered with or without simultaneous administration of cimetidine. These findings indicate that acid hypersecretion in basal and postprandial states after ACT may be attributed to hypergastrinemia and that endogenous acid is capable of releasing secretin but not effective on the release of PP. gastric acid ; intraduodenal pH ; gastrin ; secretin ; pancreatic polypeptide It is well known that plasma secretin (Koizumi et al. 1980) and PP (Kayasseh et al. 1978) concentration significantly increases as hydrochloric acid is infused into the duodenum. The effect of physiological stimulus such as meal and gastric acid on plasma secretin and PP release, however, has not been elucidated in detail.It is important to examine the effect of endogenously released gastric acid on plasma secretin and PP response for the elucidation of release mechanism and furthermore of a physiological role of these gut hormones in gastrointestinal

Gastric acid secretion, intraduodenal pH and gastroenteropancreatic hormone release to bombesin in antrocolic transposition dogs.

“…Therefore, hyperinsulinemia by bombesin infusion in ACT dogs may be the combined effect of bombesin, gastrin, secretin and gastric acid on f3-cells. Postprandial hypergastrinemia, hypersecretinemia and acid hypersecretion were reported in this ACT model (Koizumi et al 1984), but it is uncertain whether chronic endogenous hypergastrinemia, hypersecretinemia and acid hypersecretion influence the fl-cell function.…”

“…Plasma gastrin, secretin and pancreatic polypeptide (PP) were measured by radioimmunoassay as previously described (Koizumi et al 1984). Plasma motilin was also measured by radioimmunoassay using the antiserum GP1103 obtained from guinea pig serum (Shin et al 1980).…”

“…Antrocolic transposition (ACT) is an experimental model in the dog that is characterized by the coexistence of basal hypergastrinemia and acid hypersecretion by the stomach (Nasca et al 1975; Koizumi et al 1984) and simulate closely the conditions found in cases of both Zollinger-Ellison syndrome and retained excluded antrum in human beings.…”

Role of autonomic nervous system in the response of plasma pancreatic polypeptide to glycopenia in dogs.

“…Plasma was separated by centrifugation at 4°C and stored at -20©C prior to assay . Plasma immunoreactive insulin (IRI) (Morgan and Lazarow 1963), immunoreactive glucagon (IRG) (Ohneda et al 1975) and PP (Koizumi et al 1984) were measured by radioimmunoassay as described previously.…”

Plasma gastrin and pancreatic polypeptide response to atropine and sham feeding in man.

“…The plasma PP concentration was measured according to a previously described technique (Koizumi et al 1984). Antiserum against porcine PP (lot K-5418) was used at the final dilution of 1: 1,250,000, to bind approximately 50% of the added 1251-PPP Reference standards for the assay were prepared from PPP (lot 741029).…”