Effect of Maxing Shigan Tang on H1N1 Influenza A Virus-Associated Acute Lung Injury in Mice

Abstract

Objective: This study is aimed at examining the effects of Maxing Shigan Tang (MST) treatment on H1N1-associated acute lung injury (ALI) and exploring the possible mechanism. Material and Methods: Mice were randomly divided into a control group, model group, peroxisomal proliferator activator receptor γ (PPARγ) inhibition group (PPARγ-), PPARγ activation group (PPARγ+), and MST group. Influenza A (H1N1) virus of the Fort Monmouth 1 (FM1) strain was used to induce an ALI mice model. Hematoxylin and eosin staining was performed to investigate the effect of MST treatment on H1N1-associated ALI. Cell apoptosis of lung tissues of each group were conducted through transferase-mediated dUTP nick end-labeling methods. Moreover, the expression level of caspase 3, activity of caspase 3, and serum level of tumor necrosis factor (TNF)-α of each group were also analyzed. Finally, quantitative real-time polymerase chain reaction and Western blotting analysis were carried out to detect angiopoietin-like 4 (ANGPTL4) expression level. Results: We found that mice infected with the FM1 strain of H1N1 influenza A virus developed severe ALI, and MST could improve H1N1-induced ALI. Moreover, MST decreased lung cell apoptosis and reduced the serum content of TNF-α. In addition, MST significantly induced the ANGPTL4 expression in H1N1-induced ALI. Conclusion: MST improves H1N1-associated ALI maybe through targeting ANGPTL4 in mice.

Exploring the potential therapeutic effect of traditional Chinese medicine on coronavirus disease 2019 (COVID-19) through a combination of data mining and network pharmacology analysis

“…This formula has been widely applied for the treatment of colds, influenza, acute bronchitis, bronchial asthma, and chronic obstructive pulmonary disease [14] . The clinical study also showed a positive effect for the patient with H1N1 influenza virus infection [15] , and its ability to alleviate the virus-induced lung injury has attracted more and more research interest [16] , [17] . Therefore, this formula has been recommended as one of the prescriptions for the COVID-19 treatment and has been proved to be beneficial to cure respiratory tract diseases with symptoms of fever, cough, short breath, and body ache, and so on.…”

The Mechanism of Prevention and Treatment of Maxing Shigan Decoction on Pulmonary Heart Injury Caused by Novel Coronavirus Pneumonia

“…The mechanism may be related to Regulation of abnormal changes in TNF-α and IL-10 levels. Maxing Shigan Decoction improves the acute lung injury induced by H1N1 by reducing the apoptosis of lung cells and the content of TNF-α in serum [19]. Maxing Shigan Decoction can reduce the expression and release of TNF-α, IL-1β and IL-6 in alveolar lavage uid, improve lung in ammation, and resist acute lung injury caused by in uenza virus infection [20].…”

The effects of the Xijiao Dihuang decoction combined with Yinqiao powder on miRNA-mRNA profiles in mice infected with influenza a virus

“…Li et al identified 67 differentially expressed cellular miRNAs between infection with the H5N1 avian influenza virus (HPAI) and the reconstructed 1918 H1N1 influenza virus (r1918) (2:6) [ 14 ]. Influenza A/FM/1/47 (H1N1)(FM1), is commonly used by Chinese researchers to investigate the immune response and inflammatory pathogenesis during IAV infection, and to evaluate the therapeutic and pharmacological effects of Chinese herbs [ 15 – 21 ]. However, the FM1-induced general changes in the levels of miRNAs and mRNAs remain unclear.…”