Effect of mating system, flushing procedure, progesterone dose and donor ewe age on the yield and quality of embryos within a MOET program in sheep

Bari, Farida Yeasmin; Khalid, M.; Haresign, W.; Murray, A.; Merrell, B. G.

doi:10.1016/s0093-691x(99)00270-8

Cited by 42 publications

(37 citation statements)

References 26 publications

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“…The success of embryo transfer depends on several factors including the mating system, flushing procedure, progesterone dose and donor ewe age [4], donor breed [35] and age [27], seasonal treatment [34], gonadotrophin derived species preparation [5], gonadotrophins dissolving agent [11]. However, a high degree of variation in the superovulatory response continues to be the greatest problem limiting application of the embryo transfer program in field conditions [40].…”

Section: Introductionmentioning

confidence: 99%

Sheep embryos derived from FSH/eCG treatment have a lower in vitro viability after vitrification than those derived from FSH treatment

et al. 2001

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-In the non breeding period, the effect of two superovulatory treatments (eCG/FSH in single dose or FSH alone in four decreasing doses) on the production of embryo quality following in vitro viability after vitrification procedures was investigated using forty-four adult Sarda breed ewes. In sheep treated with eCG/FSH, the mean number of corpora lutea was significantly (P < 0.05) higher (11.8 ± 4.0 vs. 8.05 ± 3.8), although the recovery rate was significantly (P < 0.01) lower (74.6 vs. 59.9) than with FSH alone. After vitrification (ethylene glycol and glycerol) was repeated three times, the rates of re-expansion at first and second warming were significantly (P < 0.01) higher in embryos derived from FSH alone than in those with both gonadotrophins (94.9 and 41.9 vs. 72.8 and 18.6) and after the last vitrification the hatched blastocyst rates were 22.5 and 7.6. After differential stain, blastocysts derived from FSH alone showed a mean number of cells significantly higher than blastocysts from eCG/FSH (184.2 vs. 157.7). It was concluded that superovulatory treatment with eCG/FSH may increase the ovarian responses compared with FSH alone, but these embryos showed a reduction in viability rates after repeated vitrification. superovulation / embryo quality / ovine / vitrification / viabilityRésumé -Les embryons ovins obtenus après un traitement de superovulation associant FSH et eCG ont une viabilité in vitro plus faible après vitrification que les embryons obtenus avec un traitement séquentiel de FSH. Pendant la saison non reproductive, on a évalué, chez 44 brebis adultes de race Sarde, l'effet de deux traitements de superovulation (le premier avec les eCG/FSH en une seule administration, le deuxième avec la FSH administrée en quatre doses décroissantes) sur la qualité des embryons produits et sur la viabilité in vitro après vitrification. Dans le groupe traité avec les eCG/FSH, le nombre moyen d'ovulations était plus élevé (11,8 ± 4,0 vs. 8,05 ± 3,8, P < 0,05) tandis que le taux de récupération était plus faible (74,6 vs. 59,9, P < 0,01) par rapport au groupe traité avec la FSH seule. Les embryons ont suivi 3 vitrifications successives (éthylène glycol et glycérol). Reprod. Nutr. Dev. 41 (2001) [239][240][241][242][243][244][245][246] 239

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Section: Introductionmentioning

confidence: 99%

Sheep embryos derived from FSH/eCG treatment have a lower in vitro viability after vitrification than those derived from FSH treatment

et al. 2001

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“…According to literature, laparoscopic method allows for shortening of time of the procedure (Nellenschulte and Neimann 1992, McMillan and Hall 1994, Besenfelder et al 1997, it can be performed in form environment (Baldassarre 1999), it allows for multiple procedures in the same donors and recipients (Baril et al 1989, Wieczorek et al 2010, and it is safe for animals because of the minimal dosage and short time of infusion of anesthetic drugs (Rátky et al 2001). It decreases the risk of infectious disease transmission (Bielanski 2012) and costs connected with embryos transfer as well as it lets to obtain genetic material of good quality (Baldassarre et al 1999, Bari et al 2000.…”

Section: Discussionmentioning

confidence: 99%

Piglets born after intrauterine laparoscopic embryo transfer

Wieczorek¹,

Koseniuk²,

Mandryk³

et al. 2015

Polish Journal of Veterinary Sciences

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The aim of the study was the preliminary development of laparoscopic transfer of embryos to the uterus in the pig, which can become the alternative for more invasive surgical methods. We proposed the original method of embryo transfer. Donors (n = 40) and recipients (n = 15) of embryos were sows of age of 6-8 months. The estrus cycle of both recipients and donors was routinely synchronized. The experimental animals were divided into two groups. In the first group (10 donors and 3 recipients) embryos were transplanted according to the method described earlier and in the second group (30 donors and 12 recipients) embryos were transplanted according to our own proposed method. As the control group, we used 16 sows after insemination (AI). In animals from both experimental groups pregnancy was diagnosed between 28-31 day after transplantation and in the control group between 28-31 day after insemination. All animals were observed during pregnancy and weaning period in pig farm. Embryos at the development stage of 2-4 cell were obtained surgically and cultured in vitro for 4 days. Obtained blastocysts were transferred to donors. The original set of catheters for blastocysts transfer to pig uterus was constructed. Three trocars were placed in abdominal cavity for inserting endoscope and 2 grasps for uterus stabilization. After uterus stabilization, the slide was inserted into abdomen which was used for putting the needle to puncture uterus. Through this needle catheter with embryos was inserted into the uterus cavity. Embryos were placed by injection into lumen of the one uterine horn. From 12 recipients pregnancy was diagnosed in 6 recipients. From 6 litters, 57 piglets were born. We weaned 41 piglets (71.9%). In our study we obtained 50% efficacy, with the mean number of 9.5 alive piglets in litter and 6.8 weaned piglets. The efficacy of developed method of laparoscopic transfer of porcine embryos allows it to be used in routine practice.

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“…The method of embryo recovery used in this study differs from a previously described semilaparoscopic method (Bari et al, 2000) in which a Foley catheter and a volume of 60 ml of the medium or a combination of the medium and air is used. Some small details incorporated in the procedure used in this study like the visible blood clot removal and irrigation with heparinised saline solution reduce postsurgical adherences and can increase the number of procedures in the same ewe (Ramon et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Embryo recovery from the oviduct in superovulated ewes: a method to improve MOET systems

Ramón-Ugalde¹,

Folch

Cocero

et al. 2008

CZECH J ANIM SCI

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ABSTRACT:The efficiency of embryo recovery in a superovulatory treatment was studied by perfusing the oviduct or the uterine horn in 3.5 and 7 days after sponge withdrawal, respectively. Eighty-four and seventy ewes of Aragonesa breed were used as embryo donors and receptors, respectively. The donors were distributed in 3 replications. The oestrus was synchronized with the insertion of FGA intravaginal sponges (30 mg) for 13 days. Six intramuscular injections of 18 mg pFSH were applied in decreasing doses at 12 hour intervals starting 48 hours after sponge withdrawal. The recovery rate (RR) (83.2 vs. 75.8%), the viability rate (VR) (73.5 vs. 47.2%) and the number of viable embryos (VE) per donor ewe (5.9 ± 0.79 vs. 3.0 ± 0.37) were higher (P < 0.01) in ewes whose perfusion was done directly in oviducts. The interval from sponge withdrawal to oestrus had no influence on RR in any groups; however it had an effect on the VR in 3.5 and 7 days old embryos. Embryos from ewes showing the oestrus within 20 hours after sponge withdrawal had a higher viability. On the other hand, using morphologic criteria, 26.5% and 52.7% of 3.5 and 7 days old embryos, respectively, were discarded before transfer. The percentage of ewes in each group that became pregnant was similar in both groups. We conclude that in superovulated ewes the embryo recovery rate, viability and number of viable embryos are higher in embryos recovered from the oviduct compared to those recovered from the uterus, without decreasing the conception rate and the viability rate is higher in ewes that show the oestrus 20 hours before sponge withdrawal.

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Effect of mating system, flushing procedure, progesterone dose and donor ewe age on the yield and quality of embryos within a MOET program in sheep

Cited by 42 publications

References 26 publications

Sheep embryos derived from FSH/eCG treatment have a lower in vitro viability after vitrification than those derived from FSH treatment

Sheep embryos derived from FSH/eCG treatment have a lower in vitro viability after vitrification than those derived from FSH treatment

Piglets born after intrauterine laparoscopic embryo transfer

Embryo recovery from the oviduct in superovulated ewes: a method to improve MOET systems

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