Effect of mating combinations on the host parasitisation and sex allocation in solitary endoparasitoid, <i>Aenasius arizonensis</i> (Hymenoptera: Encyrtidae)

Shera, P. S.; Karmakar, Prasun

doi:10.1080/09583157.2017.1413707

Cited by 5 publications

(4 citation statements)

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“…As a specific and dominant endoparasitoid of P. solenopsis, A. bambawalei has the potential for immediate use in practical applications in biological control. 38,39,47,48 In this study, diethyl sebacate, 2,4,4-trimethyl-2-pentene, and 1-octen-3-one were identified as behaviorally active semiochemicals in A. bambawalei based on the interaction analysis between AbamOBP28 and 27 candidate ligands through fluorescence competitive binding and CD spectra assays in vitro, and EAG and olfactory bioassays in vivo. These compounds identified in the present work may promote the efficacy of A. bambawalei in biological control.…”

Section: Discussionmentioning

confidence: 91%

“…We also demonstrated the importance of binding mechanism analyses for OBPs contributing to improvement in the efficiency of reverse chemical ecology. As a specific and dominant endoparasitoid of P. solenopsis , A. bambawalei has the potential for immediate use in practical applications in biological control 38,39,47,48 . In this study, diethyl sebacate, 2,4,4‐trimethyl‐2‐pentene, and 1‐octen‐3‐one were identified as behaviorally active semiochemicals in A. bambawalei based on the interaction analysis between AbamOBP28 and 27 candidate ligands through fluorescence competitive binding and CD spectra assays in vitro , and EAG and olfactory bioassays in vivo .…”

Section: Discussionmentioning

confidence: 98%

“…35,37 Aenasius bambawalei Hayat (Hymenoptera: Encyrtidae) is the most aggressive endoparasitoid of P. solenopsis, displaying strong control potential for P. solenopsis. 38 In some studies, the parasitism percentage of P. solenopsis by A. bambawalei exceeded 70% in the field. 39 Parasitoids have evolved sensitive olfactory systems to recognize key semiochemicals from their environment.…”

Section: Introductionmentioning

confidence: 96%

“…It has caused serious damage to agricultural and horticultural production in Asia 35,37 . Aenasius bambawalei Hayat (Hymenoptera: Encyrtidae) is the most aggressive endoparasitoid of P. solenopsis , displaying strong control potential for P. solenopsis 38 . In some studies, the parasitism percentage of P. solenopsis by A. bambawalei exceeded 70% in the field 39 .…”

Section: Introductionmentioning

confidence: 99%

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Discovery of behaviorally active semiochemicals in Aenasius bambawalei using a reverse chemical ecology approach

Yang

Duan

et al. 2021

Pest Management Science

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BACKGROUND The invasive mealybug, Phenacoccus solenopsis, has caused serious damage to cotton crops throughout the world. Aenasius bambawalei is a dominant endoparasitoid of P. solenopsis. Exploration of behaviorally active semiochemicals may promote the efficacy of parasitoids used in biological control. Reverse chemical ecology, based on the physiological function of odorant‐binding proteins (OBPs), provides an effective approach to screen behaviorally active compounds to target insect pests. Determination of the binding mechanisms and specificity towards different odorants in A. bambawalei may facilitate the development of more‐efficient biological control strategies. RESULTS We characterized the expression profile and analyzed the binding affinity of OBP28 in A. bambawalei. AbamOBP28 showed high expression in the wings and antennae of both male and female A. bambawalei. A fluorescence competitive binding assay indicated that AbamOBP28 displayed strong binding affinity to most candidate ligands. Circular dichroism spectra demonstrated that 1‐octen‐3‐one, myrcene, dodecane, 2,4,4‐trimethyl‐2‐pentene, nonanal, and limonene elicited conformational changes in AbamOBP28. Electrophysiological and behavioral bioassays revealed that diethyl sebacate, 2,4,4‐trimethyl‐2‐pentene, and 1‐octen‐3‐one evoked significant electroantennography responses and functioned as attractants in A. bambawalei at specific concentrations. Furthermore, three‐dimensional structure modeling and molecular docking showed that hydrogen bonds were formed by Glu1 and Ser75 of AbamOBP28 with diethyl sebacate, respectively. CONCLUSION These results demonstrate that AbamOBP28 is involved in the chemoreception of A. bambawalei. The identified protein provides a potential target for efficient enemy utilization and pest control, and the overall results may help develop protocols for more effective screening of behaviorally active semiochemicals. © 2021 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 98%