Effect of manganese supplementation on the membrane integrity and the mitochondrial potential of the sperm of grazing Nelore bulls

Reis, L. S. L. S.; Ramos, A. A.; Camargos, A. S.; Oba, Eunice

doi:10.1016/j.anireprosci.2014.06.033

Cited by 15 publications

(8 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For Se, addition of this mineral to semen extender increased functional membrane integrity of frozen–thawed buffalo sperm (Dorostkar et al ., 2012). Recently, Reis et al (2014) showed that membrane integrity of Nellore sperm was increased by dietary Mn supplementation. In vitro , Mn supplementation of bovine semen during cryopreservation showed a protective effect, growing the percentage of HOS-positive spermatozoa (Cheema et al ., 2009).…”

Section: Discussionmentioning

confidence: 99%

The importance of trace minerals copper, manganese, selenium and zinc in bovine sperm–zona pellucida binding

Anchordoquy

Lizarraga

et al. 2019

Zygote

View full text Add to dashboard Cite

SummarySperm–zona pellucida (ZP) binding is a necessary event for successful fertilization. The aim of this study was to determine the effect of trace minerals such as copper (Cu), manganese (Mn), selenium (Se) and zinc (Zn) on bovine spermatozoa binding to ZP. Sperm viability, functional membrane integrity, acrosomal status (AS), total antioxidant capacity (TAC) and sperm lipid peroxidation (LPO) were also evaluated. For the present study, in vitro fertilization (IVF) medium was supplemented with Cu (0.4 µg/ml Cu), Mn (5 ng/ml Mn), Se (100 ng/ml Se), Zn (0.8 µg/ml Zn), all minerals (Cu+Mn+Se+Zn), or tested without supplement (Control). Considerably more sperm bound to ZP when Cu, Se or Zn were added to the IVF medium, but there were no difference compared with the Control, Mn and Cu+Mn+Se+Zn groups. After 1 h of incubation, viability was increased by the addition of Cu, Mn and Se with respect to the Control but, after 2 h, viability was higher only with the addition of Mn to IVF medium. Functional membrane integrity improved in sperm treated with Cu. Acrosome integrity was higher in sperm treated with Zn after 1 h of incubation. LPO was significantly higher in sperm treated with Cu or Cu+Mn+Se+Zn. The mean TACs of sperm treated with Cu, Mn, Zn or Cu+Mn+Se+Zn were lower than in the Control. In conclusion, the results obtained in the present study determined that the presence of Cu, Se and Zn in the IVF medium increased the number of spermatozoa bound to the ZP, highlighting the importance of these minerals in the fertilization process.

show abstract

Section: Discussionmentioning

confidence: 99%

The importance of trace minerals copper, manganese, selenium and zinc in bovine sperm–zona pellucida binding

Anchordoquy

Lizarraga

et al. 2019

Zygote

View full text Add to dashboard Cite

show abstract

“…Twice during each study period, semen samples were collected by electroejaculation because the bulls were reared in an extensive system of pastures to promote natural mating and were not adapted to semen harvesting through an artificial vagina as previously done by Freneau et al (2006), Fernandes et al (2008), and Reis et al (2014).…”

Section: Methodsmentioning

confidence: 99%

Integrity of the plasma membrane, the acrosomal membrane, and the mitochondrial membrane potential of sperm in Nelore bulls from puberty to sexual maturity

Reis

Ramos

Camargos

et al. 2016

Arq. Bras. Med. Vet. Zootec.

Self Cite

View full text Add to dashboard Cite

This study evaluated the plasma membrane integrity, acrosomal membrane integrity, and mitochondrial membrane potential of Nelore bull sperm from early puberty to early sexual maturity and their associations with sperm motility and vigor, the mass motility of the spermatozoa (wave motion), scrotal circumference, and testosterone. Sixty Nelore bulls aged 18 to 19 months were divided into four lots (n=15 bulls/lot) and evaluated over 280 days. Semen samples, collected every 56 days by electroejaculation, were evaluated soon after collection for motility, vigor and wave motion under an optical microscope. Sperm membrane integrity, acrosomal integrity, and mitochondrial activity were evaluated under a fluorescent microscope using probe association (FITC-PSA, PI, JC-1, H342). The sperm were classified into eight integrity categories depending on whether they exhibited intact or damaged membranes, an intact or damaged acrosomal membrane, and high or low mitochondrial potential. The results show that bulls have a low amount of sperm with intact membranes at puberty, and the sperm show low motility, vigor, and wave motion; however, in bulls at early sexual maturity, the integrity of the sperm membrane increased significantly. The rate of sperm membrane damage was negatively correlated with motility, vigor, wave motion, and testosterone in the bulls, and a positive correlation existed between sperm plasma membrane integrity and scrotal circumference. The integrity of the acrosomal membrane was not influenced by puberty. During puberty and into early sexual maturity, bulls show low sperm mitochondrial potential, but when bulls reached sexual maturity, high membrane integrity with high mitochondrial potential was evident.Keywords: sexual development, sperm motility, sperm vigor, testosterone, cattle RESUMO

show abstract

“…Studies with livestock have also reported the importance of adding adequate levels of micronutrients to the diet of cattle. Diets deficient in Mn may interfere with fertility (Carvalho et al, 2010), while its excess can cause poisoning and affect the reproductive performance (Reis et al, 2014). Given the importance of establishing appropriate levels of manganese in forage species used in livestock worldwide, highlighting Brachiaria (Syn.…”

Section: Introductionmentioning

confidence: 99%

Growth, nutrition and production of dry matter of Kikuyu Grass (Brachiaria humidicula) as a function of Mn-fertilizer

Arruda¹,

Flores²,

Damin³

et al. 2016

Aust J Crop Sci

View full text Add to dashboard Cite

Manganese (Mn) is important to increase forage crop yields. However, there is little information regarding the adequate Mn-fertilizer rates for Brachiaria humidicula species. The objective of this research was to evaluate the effect of manganese on growth, nutrition and yield of Brachiaria humidicula. The study was carried out in a green house in a randomized block design with five rates of manganese (0, 15, 30, 60 and 120 mg dm -3 ) and four replicates. Were evaluated plant height, leaf area, relative chlorophyll index, dry matter production, manganese accumulation and content, besides absorption efficiencies and transport and use of manganese (Mn). Brachiaria humidicula showed high tolerance to this nutrient, because the application of only 120 mg dm -3 to the soil was phytotoxic, showing symptoms such as brown spots and leaf tip curling. Manganese applied at a dose of 120 mg dm -3 reduced aerial part biomass yield by 25% and promoted lower efficiency of use of this nutrient by the forage by 49%. However, even with the initial content of manganese in the soil considered sufficient to meet nutritional demands to achieving high yields, the application of 60 mg dm -3 of manganese to the soil is recommended.

show abstract

Effect of manganese supplementation on the membrane integrity and the mitochondrial potential of the sperm of grazing Nelore bulls

Cited by 15 publications

References 7 publications

The importance of trace minerals copper, manganese, selenium and zinc in bovine sperm–zona pellucida binding

The importance of trace minerals copper, manganese, selenium and zinc in bovine sperm–zona pellucida binding

Integrity of the plasma membrane, the acrosomal membrane, and the mitochondrial membrane potential of sperm in Nelore bulls from puberty to sexual maturity

Growth, nutrition and production of dry matter of Kikuyu Grass (Brachiaria humidicula) as a function of Mn-fertilizer

Contact Info

Product

Resources

About