Effect of manassantin B, a lignan isolated from<i>Saururus chinensis</i>, on lipopolysaccharide-induced interleukin-1β in RAW 264.7 cells

Park, Hwan Chul; Bae, Hong-Beom; Chang-hyun, Jeong; Lee, Seong Heon; Jeung, Hye Jin; Kwak, Sang-Hyun

doi:10.4097/kjae.2012.62.2.161

Cited by 9 publications

(5 citation statements)

References 27 publications

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“…These include the nuclear factor -light-chain-enhancer of activated B cells (NF-B) (3)(4)(5)(6) and CCAAT/enhancer-binding protein ␤ transcription factors (7), the mitogen-activated protein kinase (MAPK) (6,8,9), interleukin 6 -induced signal transducer and activator of transcription 3 (STAT3) (10), and hypoxia-inducible factor 1␣ (HIF-1␣) 2 (11)(12)(13). Some of these "molecular targets" were observed only in selected cell lines (7,8,10). It is unclear whether these biochemically diverse molecules or pathways are direct targets of manassantin or secondary cellular responses.…”

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confidence: 99%

“…Moreover, it remains unknown how these effectors contribute to the broad spectrum of medical benefits of manassantin seen in humans. Systematic screening via large-scale protein folding and stability measurements identified a number of hits that did not significantly overlap with those found from random cell-based analyses (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(15)(16)(17)(18)(19)(20).…”

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The lignan manassantin is a potent and specific inhibitor of mitochondrial complex I and bioenergetic activity in mammals

Min

Oh³

et al. 2017

Journal of Biological Chemistry

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Dineolignans manassantin A and B from the plant are used in traditional medicine to manage a wide range of ailments such as edema, jaundice, and gonorrhea. Cell-based studies have identified several molecular target candidates of manassantin including NF-κB, MAPK, STAT3, and hypoxia-inducible factor 1α (HIF-1α). It is unclear whether or how these structurally diverse proteins or pathways mediate any of the medical benefits of manassantin Moreover, it has recently been reported that manassantin causes developmental arrest in zebrafish by inhibiting the mitochondrial complex I, but it is unknown whether manassantin inhibits mitochondrial respiration in intact mammalian cells and live animals. Here, we present direct evidence that manassantin potently and specifically inhibits the mitochondrial complex I and bioenergetic activity in mammalian systems. Manassantin had no effect on complex II- or complex IV-mediated respiration. Of note, it decreased NADH-ubiquinone reductase activity but not the activity of NADH-ferricyanide reductase. Treatment with manassantin reduced cellular ATP levels and concomitantly stimulated AMP-activated protein kinase and As an adaptive response to manassantin-induced bioenergetic deficiency, mammalian cells up-regulated aerobic glycolysis, a process mediated by AMP-activated protein kinase (AMPK) independently of HIF-1α. Together these results demonstrate a biologically important activity of manassantin in the control of complex I-mediated respiration and its profound effects on oxygen utilization, energy homeostasis, and glucose metabolism in mammalian cells.

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confidence: 99%

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confidence: 99%

The lignan manassantin is a potent and specific inhibitor of mitochondrial complex I and bioenergetic activity in mammals

Min

Oh³

et al. 2017

Journal of Biological Chemistry

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“…MB, a neolignan isolated from the roots of S. chinensis, has been demonstrated to exhibit a range of activities, including anti-inflammatory (10,(19)(20)(21), antiseptic (22) and antitumor activities (11,23). Furthermore, MB was shown to inhibit PMA-induced ICAM-1 expression in HL-60 cells (15) and to prevent monocyte adhesion to HUVECs through the inhibition of ICAM-1, VCAM-1 and E-selectin expression stimulated by TNF-α (24).…”

Section: Discussionmentioning

confidence: 99%

The dineolignan from Saururus chinensis, manassantin B, inhibits tumor-induced angiogenesis via downregulation of matrix metalloproteinases 9 in human endothelial cells

Liu

et al. 2014

Oncology Reports

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Abstract. Manassantin B (MB) is a neolignan isolated fromSaururus chinensis that exhibits a range of activities, including anti-inflammatory, antiseptic and antitumor activity. MB was recently found to affect cell adhesion and expression of several adhesion molecules. Based on the important roles of these adhesion molecules in angiogenesis, we evaluated a possible role for MB in tumor-induced angiogenesis in endothelial cells (ECs). In the present study, we found that MB blocked tumor-induced tube formation of ECs and significantly inhibited the invasion of ECs through the reconstituted basement membrane. MB suppressed the activity of matrix metalloproteinases (MMPs) and downregulated the expression of matrix metalloproteinases 9. Western blotting showed reduction of RUNX2 activation by MB. RUNX2 transcription factor assay and chromatin immunoprecipitation assay showed that the interaction between RUNX2 and target sequences in the matrix metalloproteinases 9 promoters was inhibited by MB. Our findings suggested that the inhibitory effects of MB on tumor-induced angiogenesis were caused by matrix metalloproteinases 9 inhibition, which was associated with the downregulation of RUNX2 transcriptional activity. IntroductionAngiogenesis, the development of novel blood vessels, is a critical event in many important disease states including ischemic cardiovascular disease, wound healing, inflammation, psoriasis, primary tumor growth and formation of metastases. It is a highly complex process involving endothelial cell (EC) activation, disruption of vascular basement membranes, and migration and proliferation of ECs (1). This process is orchestrated by a large number of cytokines and associated receptors and proteinases, such as vascular endothelial growth factor (VEGF), fibroblast growth factor, interleukin 8 and matrix metalloproteinases (MMPs) (2). Tumor-induced angiogenesis is a process of irregular blood vessel formation, which results in structurally and functionally abnormal blood vessels. This abnormality impairs effective delivery of therapeutic agents to all regions of tumors, it creates abnormal properties of cancer cells, such as survival, resistance of radiation and chemotherapy, and selects for more malignant cells (3). Several decades ago, Dr Folkman proposed anti-angiogenic therapy as an effective method for treatment of cancer (4,5). Nowadays, a number of inhibitors targeting the tumor vasculature have been identified to improve the sensitivity of cancer cells to cytotoxic chemotherapy and other therapeutics (6-8).Saururus chinensis (S. chinensis) is a perennial herbaceous plant that grows wild in moist and shady places throughout East Asia (9). It has a long history of use in China as a folk medicine to remedy various edema, gonorrhea, jaundice and inflammatory diseases (9-11). Previous phytochemical and pharmacological studies on this plant have revealed several types of secondary metabolites, such as lignans, neolignans, aristolactams and flavonoids, as the biologically active compounds (12,13). Numer...

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“…Manassantin B (MB) is a neolignan constituent of the roots of Saururus chinensis, which is used in the treatment of gonorrhea, edema, and jaundice in Korean traditional medicine [13]. According to previous studies, MB exerts anti-tumor [14,15], anti-inflammatory [16,17], anti-melanogenic [18,19], anti-human immunodeficiency virus [20], and anti-obesity [21,22] activities. In addition, MB exhibits an anti-tumor effect by inhibiting cell aggregation via the inhibition of phorbol 12-myristate 13-acetate-induced intercellular cell adhesion molecule-1 expression in HL-60 cells [14].…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effect of Manassantin B Isolated from Saururus chinensis on Skin Heat Aging

Ryu¹,

Choi²,

Lee³

et al. 2020

Cosmetics

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Heat shock treatment-induced skin aging causes a thickened epidermis, increased matrix metalloproteinase (MMP)-1 expression, collagen degradation, and deep wrinkles. In this study, we investigated the effect of manassantin B in preventing heat shock treatment-induced aging. We first separated manassantin B (MB) from the roots of Saururus chinensis, and the structure was identified using 1H- and 13C-NMR spectroscopy. RT-PCR and western blotting were applied to investigate the anti-aging effect of manassantin B. Manassantin B decreased MMP-1 expression through transient receptor potential vanilloid (TRPV) 1 channel inhibition and significantly increased procollagen expression. In addition, manassantin B suppressed MAPK phosphorylation in a dose-dependent manner. Our results suggest that manassantin B, the active ingredient in S. chinensis, can be effectively used to inhibit heat shock treatment-induced skin aging.

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Effect of manassantin B, a lignan isolated fromSaururus chinensis, on lipopolysaccharide-induced interleukin-1β in RAW 264.7 cells

Cited by 9 publications

References 27 publications

The lignan manassantin is a potent and specific inhibitor of mitochondrial complex I and bioenergetic activity in mammals

The lignan manassantin is a potent and specific inhibitor of mitochondrial complex I and bioenergetic activity in mammals

The dineolignan from Saururus chinensis, manassantin B, inhibits tumor-induced angiogenesis via downregulation of matrix metalloproteinases 9 in human endothelial cells

Inhibitory Effect of Manassantin B Isolated from Saururus chinensis on Skin Heat Aging

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