Effect of Mailuoning injection on 8-iso-prostaglandin F2 alpha and superoxide dismutase in rabbits with extremity ischemia–reperfusion injury

Wang, Daijun; Tian, Hua

doi:10.1016/j.jss.2014.06.008

Cited by 13 publications

(5 citation statements)

References 31 publications

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“…MDA is the end product of lipid oxidation, and also a parameter indicating the formation of ROS (reactive oxygen species) in postischemic tissue (Wang & Tian, ). The treatment with the fractions of hydrolysate reduced the MDA level of the cells significantly ( p < .05) when compared with the model group.…”

Section: Resultsmentioning

confidence: 99%

Radical scavenging activities of peptide from Asian clam ( Corbicula fluminea ) and its protective effects on oxidative damage induced by hydrogen peroxide in HepG2 cells

Hao

Jiang

et al. 2020

J Food Biochem

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The soft part of Asian clam (Corbicula fluminea) was hydrolyzed using trypsin and the hydrolysates were separated by ultrafiltration using the membrane with molecular weight cutoff of 10 and 5 kDa. Three fractions (F10000, F10000–5000, and F5000) were obtained. The F5000 showed the strongest scavenging abilities to DPPH (2,2‐diphenyl‐1‐picrylhydrazyl), superoxide anion, and hydroxyl radicals, and was further fractionated into four fractions (component I, II, III, and IV) by gel chromatography. The protective effects of these components on oxidative damage induced by hydrogen peroxide (H2O2) in HepG2 cells were evaluated. Meanwhile, component I separated from F5000 had more positive effects on increasing the activity of catalase, decreasing lipid peroxidation, inhibiting H2O2‐induced apoptosis, and higher yield. The component I was further separated using anion exchange chromatography and reversed phase high‐performance liquid chromatography, and the peptide sequence of Lys‐Gly‐Pro‐Ala‐ Pro‐Phe‐Tyr‐Pro‐Leu was identified by mass with molecular weight of 988.3 Da. Practical applications Asian clam (C. fluminea) is used for liver protection in traditional Chinese medicine. The present study investigated the radical scavenging activity of the hydrolysates from C. fluminea and the protective effects of the hydrolysate fractions on oxidative damage induced by H2O2 in HepG2 cells. In addition, a peptide was purified from the hydrolysate and the amino acid sequence of it was identified. Although chemical antioxidant has some side effects on health, the peptide with antioxidant activity obtained from C. fluminea would have more extensive application in food and nutraceutical.

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Section: Resultsmentioning

confidence: 99%

Radical scavenging activities of peptide from Asian clam ( Corbicula fluminea ) and its protective effects on oxidative damage induced by hydrogen peroxide in HepG2 cells

Hao

Jiang

et al. 2020

J Food Biochem

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“…Growing evidence showed that oxidative stress plays critical role in hepatic damage and responsible for hepatocyte death after I/R (Somuncu, Cakmak, Dikmen, Akman, & Kaya, ). MDA, the end product of lipid peroxidation, is regard as a well‐known parameter to determine the formation of reactive oxygen species (ROS) in postischemic tissue (Wang & Tian, ). SOD, an enzymatic antioxidant, whose primary role is within the antioxidant defense mechanism, reduce or completely eliminate the hazardous effects of ROS.…”

Section: Resultsmentioning

confidence: 99%

Protective effects of tilapia fish oil and liposomes on ischemia reperfusion injury of rat liver

Lin

Wang

et al. 2018

J Food Biochem

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The effects of tilapia fish oil and liposomes containing fish oil on hepatic ischemia/reperfusion (I/R) injury of rats were investigated. The activities of alanine aminotransferase, aspartate aminotransferase, gamma glutamyl transferase and lactate dehydrogenase, and the levels of tumor necrosis factor‐alpha, interleukin‐6, and interleukin‐10 in serum of rats were determined. In addition, the malondialdehyde contents, superoxide dismutase activities, nuclear factor‐kappaB (NF‐кB) activation and the apoptosis in hepatic tissue of rats were estimated. The results showed that the administration of tilapia fish oil and liposomes could reduce the hepatocellular damage, neutrophil infiltration and oxidative stress, decrease inflammatory cytokines, increase antiinflammatory cytokine, and attenuate NF‐кB activation of hepatic I/R injury rats. Overall, the tilapia fish oil and liposomes exerted antiinflammatory and antioxidant effects on ischemic liver, thereby protecting hepatocytes against I/R injury. Practical applications Fish oil has many dietary benefits, but due to its strong odors and rapid deterioration, its application in food formulations is limited. The encapsulation of fish oil in liposomes is a good choice for reduction of fish odor smell and improvement of oxidative stability. The present study investigated the protective effects of tilapia fish oil and liposomes on hepatic I/R injury of rat. The results showed that both the free tilapia fish oil and its liposomes had antiinflammatory and antioxidant effects on the experimental rats. Therefore, liposomes containing tilapia fish oil should be considered as a prospective functional food and pharmaceuticals application.

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“…Hind limb ischemia was induced by clamping the common iliac artery with a vascular clamp (SukitaClip; Mizuho Medical Ca., Bunkyoku, Japan) and by wrapping a rubber tourniquet around the proximal thigh to completely occlude collateral circulation. IR injury was established according to a method described previously [16]. …”

Section: Methodsmentioning

confidence: 99%

“…Although the mechanisms underlying IR injury are complex and not thoroughly understood, reactive oxygen species play an important role in the IR injury mechanism and antioxidant enzymes have protective effects from IR injury [15,16]. We examined three antioxidant enzymes, glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT).…”

Section: Introductionmentioning

confidence: 99%

Remote Ischemic Preconditioning Enhances the Expression of Genes Encoding Antioxidant Enzymes and Endoplasmic Reticulum Stress-Related Proteins in Rat Skeletal Muscle

Park

Kim

Cho

et al. 2016

VSI

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Purpose:Ischemic preconditioning (IPC), including remote IPC (rIPC) and direct IPC (dIPC), is a promising method to decrease ischemia-reperfusion (IR) injury. This study tested the effect of both rIPC and dIPC on the genes for antioxidant enzymes and endoplasmic reticulum (ER) stress-related proteins.Materials and Methods:Twenty rats were randomly divided into the control and study groups. In the control group (n=10), the right hind limb was sham-operated. The left hind limb (IscR) of the control group underwent IR injury without IPC. In the study group (n=10), the right hind limb received IR injury after 3 cycles of rIPC. The IscR received IR injury after 3 cycles of dIPC. Gene expression was analyzed by Quantitative real-time polymerase chain reaction from the anterior tibialis muscle.Results:The expression of the antioxidant enzyme genes including glutathione peroxidase (GPx), superoxide dismutase (SOD) 1 and catalase (CAT) were significantly reduced in IscR compared with sham treatment. In comparison with IscR, rIPC enhanced the expression of GPx, SOD2, and CAT genes. dIPC enhanced the expression of SOD2 and CAT genes. The expression of SOD2 genes was consistently higher in rIPC than in dIPC, but the difference was only significant for SOD2. The expression of genes for ER stress-related proteins tended to be reduced in IscR in comparison with sham treatment. However, the difference was only significant for C/EBP homologous protein (CHOP). In comparison with IscR, rIPC significantly up-regulated activating transcription factor 4 and CHOP, whereas dIPC up-regulated CHOP.Conclusion:Both rIPC and dIPC enhanced expression of genes for antioxidant enzymes and ER stress-related proteins.

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Effect of Mailuoning injection on 8-iso-prostaglandin F2 alpha and superoxide dismutase in rabbits with extremity ischemia–reperfusion injury

Cited by 13 publications

References 31 publications

Radical scavenging activities of peptide from Asian clam ( Corbicula fluminea ) and its protective effects on oxidative damage induced by hydrogen peroxide in HepG2 cells

Radical scavenging activities of peptide from Asian clam ( Corbicula fluminea ) and its protective effects on oxidative damage induced by hydrogen peroxide in HepG2 cells

Protective effects of tilapia fish oil and liposomes on ischemia reperfusion injury of rat liver

Remote Ischemic Preconditioning Enhances the Expression of Genes Encoding Antioxidant Enzymes and Endoplasmic Reticulum Stress-Related Proteins in Rat Skeletal Muscle

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