Effect of magnesium sulfate on the calcium-stimulated adenosine triphosphatase activity and lipid peroxidation of red blood cell membranes from preeclamptic women

Abad, Cilia; Teppa-Garrán, Alejandro; Proverbio, Teresa; Piñero, Sandy; Proverbio, Fulgencio; Marı́n, Reinaldo

doi:10.1016/j.bcp.2005.09.009

Cited by 33 publications

(34 citation statements)

References 49 publications

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“…The activity of membrane-bound enzymes dependent on proteinlipid interactions, such as the calcium adenosine triphosphatase (Ca-ATPase) of cell membranes are inhibited by increased levels of lipid peroxidation of the membranes [9]. In this sense, we found the same behavior: a lowered activity of the Ca-ATPase of red blood cell membranes of pregnant women with intrauterine growth restriction ( Table 2).…”

Section: Conjugated Dienessupporting

confidence: 60%

“…Osmotic fragility of red blood cells was determined as described elsewhere [9]. Briefly: 7.5 µl of previously washed red blood cells (50% hematocrit) were added to 1.5 ml of NaCl solutions with different osmolarities, starting with 150 mOsM.…”

Section: Osmotic Fragility Of Red Blood Cellsmentioning

confidence: 99%

“…The Ca-ATPase activity of red blood cell ghosts was determined as described elsewhere [9]. The enzymatic reaction was carried out at 37 °C, pH 7,5, with ~1.5 mg protein/ml.…”

Section: Atpase Assaysmentioning

confidence: 99%

“…The amount of lipid peroxidation of red blood cell ghosts was estimated by measuring both: TBARS and conjugated dienes as mentioned elsewhere [9]. The TBARS values were calculated using a malondialdehyde (MDA) standard curve, prepared by acid hydrolysis of 1,1,3,3-tetramethoxypropane.…”

Section: Lipid Peroxidation Measurementsmentioning

confidence: 99%

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IUGR, lipid peroxidation, and calcium ATPase activity of maternal red blood cell ghosts

Malpica¹,

Piñero²,

Chiarello³

et al. 2017

J Pregnancy Reprod

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Intrauterine growth restriction (IUGR) leads to developmental disorders and deaths among neonates. There is strong evidence of oxidative stress in newborns, as well as in placenta of pregnant women with IUGR. Maternal red blood cells can be peroxidized as they pass through the placenta, which might induce alterations reducing their fluidity and, consequently, increasing their fragility to osmotic changes and inhibiting the activity of membrane-bound enzymes, such as the Ca-ATPase.Objective: To determine osmotic fragility, lipid peroxidation levels and Ca-ATPase activity of red blood cell ghosts from pregnant women with intrauterine growth restriction in their third trimester of gestation.Methodology: Venous blood was drawn from pregnant women, either with IUGR or controls. Osmotic fragility of the red cells, as well as lipid peroxidation (TBARS and conjugated dienes) and Ca-ATPase activity of their membranes were determined.Results: It was found a higher level of osmotic fragility and lipid peroxidation, as well as a lower Ca-ATPase activity of red blood cells from the pregnant women with IUGR, as compared to control pregnant women. Conclusion:There is a clear relationship between IUGR and higher levels of osmotic fragility and lipid peroxidation, as well as a lower Ca-ATPase activity of red blood cells.

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Section: Conjugated Dienessupporting

confidence: 60%

Section: Osmotic Fragility Of Red Blood Cellsmentioning

confidence: 99%

“…The Ca-ATPase activity of red blood cell ghosts was determined as described elsewhere [9]. The enzymatic reaction was carried out at 37 °C, pH 7,5, with ~1.5 mg protein/ml.…”

Section: Atpase Assaysmentioning

confidence: 99%

Section: Lipid Peroxidation Measurementsmentioning

confidence: 99%

See 2 more Smart Citations

IUGR, lipid peroxidation, and calcium ATPase activity of maternal red blood cell ghosts

Malpica¹,

Piñero²,

Chiarello³

et al. 2017

J Pregnancy Reprod

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show abstract

“…Red blood cells of premature infants exhibit a high susceptibility to oxidative stress at birth, and the enlarged tiobarbituric acid reactive species (TBARS) presence in erythrocyte ghosts of mothers and their preterm neonates has been documented [30] . Our earlier data revealed a 2-fold increase of TBARS content in the control erythrocyte membranes in comparison to Mg-treated samples, indicating ongoing oxidative stress [13] .…”

Section: Discussionmentioning

confidence: 99%

Changes in Erythrocyte Glutathione and Plasma Membrane Calcium Pump in Preterm Newborns Treated Antenatally with MgSO<sub>4</sub>

Żylińska

Gulczyńska²,

Kozaczuk³

2008

Neonatology

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Background: Magnesium is required for the proper activity of many metabolic pathways in every cell type. Mg deficiency gives rise to preterm birth and low body weight that are associated with pathological circumstances, including disturbed ions homeostasis and insufficient antioxidant protection. Antenatal MgSO₄ treatment has been reported to exhibit a protective effect on the developing fetus; however, the molecular mechanism of this protection remains not fully understood. Objectives: Since Mg ions very rapid cross the placenta, our study tested the hypothesis whether prenatal exposure to MgSO₄ may modify the expression and activity of erythrocyte plasma membrane calcium pump (PMCA), and total erythrocyte glutathione (GSH) concentration in preterm newborns. Methods: Immunocharacteristics of erythrocyte PMCA in control and MgSO₄-treated newborns were done using general and isoform-specific antibodies. The hydrolytic activity of PMCA was determined with and without calmodulin. Total GSH erythrocyte levels were analyzed by a quantitative assay using Ellman reagent. All assays were done after delivery and 24 h later. Results: Significant differences were present in PMCA amount, isoform composition, basal activity and sensitivity for stimulation by calmodulin in both groups of neonates. The total GSH content altered during examined time, but only in the control group. Conclusions: Prenatal treatment with MgSO₄ may exert a significant influence on calcium homeostasis and nonenzymatic antioxidant reserve in the erythrocytes of preterm newborns.

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Lipid peroxidation in the liver of rats treated with V and/or Mg in drinking water

Ścibior

Zaporowska

Niedźwiecka

2009

J of Applied Toxicology

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The effect of V(5+) and Mg treatment on spontaneous and stimulated lipid peroxidation (LPO) was studied in liver supernatants obtained from outbred 5-month-old, albino male Wistar rats. The 2-month-old animals daily received deionized water to drink (control, group I); group II - water solution of NaVO(3) (SMV) at a concentration of 0.125 mg V ml(-1); group III - water solution of MgSO(4) (MS) at a concentration of 0.06 mg Mg ml(-1), group IV - water solution of SMV-MS at the same concentrations as in groups II and III for V and Mg, respectively, over a 12-week period. Three metal salts were selected as agents that may modify the LPO process (FeSO(4), NaVO(3) and MgSO(4)). V-intoxicated rats and those treated with V and Mg in combination had higher liver spontaneous malondialdehyde (MDA) formation, compared with the control and Mg-supplemented animals. In the same groups of animals the total antioxidant status (TAS) was also significantly lowered, in comparison with the control. In the supernatants obtained from the above-mentioned groups of rats a significant increase in MDA concentration was found in the presence of exogenous 30 microm FeSO(4) as well as 30, 100, 200 and 400 microm NaVO(3), compared with groups I and III. Significantly elevated MDA production was also observed in the supernatants obtained from the rats exposed to V and Mg in combination in the presence of exogenous 100 and 200 microm MgSO(4) in comparison with the control and group III as well as in the presence of exogenous 400 and 600 microm MgSO(4) compared only with group III. In vitro treatment with 1000 microm MgSO(4 )of control liver supernatants and those obtained from group III significantly enhanced MDA level, compared with spontaneous MDA formation. The two-way ANOVA indicated that the changes in the basal MDA level and in TAS in the rats at combined V and Mg application, were not due to V-Mg interaction, but resulted from independent action of V. In addition, the three-way ANOVA revealed that the changes in LPO induced by in vitro treatment of liver supernatants with exogenous Fe or V or Mg (600, 800 and 1000 microm) were a consequence of independent action of those metals and they also resulted from the interactions between Fe(exog) and V(end) and between V(end) and V(exog). In conclusion, V consumed by the rats with drinking water at a dose of 12 mg V kg(-1) body weight per 24 h for 12 weeks decreased TAS and enhanced spontaneous LPO in the hepatic tissue, which confirms its pro-oxidant potential, was also found in in vitro conditions with regard to LPO. Mg administered to rats in combination with V, at the concentration used, neither reduced nor intensified the basal LPO, compared with V-only treated animals; however, its stimulating effect on LPO was revealed in in vitro conditions, which requires further study.

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Effect of magnesium sulfate on the calcium-stimulated adenosine triphosphatase activity and lipid peroxidation of red blood cell membranes from preeclamptic women

Cited by 33 publications

References 49 publications

IUGR, lipid peroxidation, and calcium ATPase activity of maternal red blood cell ghosts

IUGR, lipid peroxidation, and calcium ATPase activity of maternal red blood cell ghosts

Changes in Erythrocyte Glutathione and Plasma Membrane Calcium Pump in Preterm Newborns Treated Antenatally with MgSO<sub>4</sub>

Lipid peroxidation in the liver of rats treated with V and/or Mg in drinking water

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