Effect of lysozyme or antibiotics on faecal zoonotic pathogens in nursery pigs

Wells, John W.; Berry, Elaine D.; Kalchayanand, Norasak; Rempel, Lea A.; Kim, M.; Oliver, W. T.

doi:10.1111/jam.12803

Cited by 23 publications

(29 citation statements)

References 43 publications

(106 reference statements)

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“…reported that dietary supplementation with 90 mg kg −1 lysozyme is as effective as antibiotics in improving growth performance and non‐specific immunity of weanling piglets. Lysozyme can take part in the natural defence mechanism in Gram‐positive bacteria and perhaps indirectly affect Gram‐negative bacteria . It was proven that lysozyme could partially replace antibiotics as an important non‐specific immune component .…”

Section: Introductionmentioning

confidence: 99%

“…Lysozyme can take part in the natural defence mechanism in Gram-positive bacteria 13 and perhaps indirectly affect Gram-negative bacteria. 14 It was proven that lysozyme could partially replace antibiotics as an important non-specific immune component. 6,15 Furthermore, Ginsburg 16 found that lysozyme has many other biological functions, including regulation of inflammatory response, and antiviral and antitumor activities.…”

Section: Introductionmentioning

confidence: 99%

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Effects of dietary lysozyme levels on growth performance, intestinal morphology, immunity response and microbiota community of growing pigs

et al. 2018

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of dietary lysozyme levels on growth performance, intestinal morphology, immunity response and microbiota community of growing pigs

et al. 2018

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“…Enteric infections caused by pathogens, like Enterotoxigenic Escherichia coli (ETEC), have a significant negative effect on neonatal survival and animal health in swine production [1, 2, 3]. Animal infants infected by pathogenic bacteria often suffer from persistent diarrhea and serious inflammation [3, 4].…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that lysozyme may possess an anti-inflammatory effect via inhibiting JNK phosphorylation [23]. Furthermore, lysozyme is capable of enhancing intestinal SIgA secretion, cause macrophage activation, and promote rapid clearance of bacterial pathogens [2, 13, 22].…”

Section: Introductionmentioning

confidence: 99%

“…The most important characteristics for any antimicrobial feed additives are weight gain and feed efficiency. Recent studies reported that lysozyme sourced from chicken eggs showed significant advances in improving growth performance, intestinal morphology, gut microbiota composition, and immunity of piglets [1, 2, 15, 24, 25]. For instance, weaned piglets received a hen-egg white lysozyme treatment shown better intestinal growth and development, as well as decreased ETEC counts on the intestinal mucosa and serum proinflammatory cytokines [26].…”

Section: Introductionmentioning

confidence: 99%

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Dietary lysozyme supplement alters serum biochemical makers and milk metabolite profile of sows via gut microbiota

Zhou

Xiong

Zou

et al. 2018

Preprint

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24Lysozyme is an important antimicrobial agent with promising future in replacing antibiotics in 25 livestok production.The aim of current study was to determine variations in sow's gut microbiota, 26 serum immunity and breast milk metabolite profile mediated by lysozyme 27 supplementation.Thirty-six pregnant sows were assigned to a control group without 28 supplementation and two treatments with 0.5 g/kg and 1.0 g/kg lysozyme provided in formula feed 29 for 21days. Microbiota analysis based on 16s RNA high-throughput sequencing and untargeted 30 liquid chromatography tandem mass spectrometry were applied and combined in analysis. Serum 31 biochemical indicators and immunoglobulins were also determined.Sows received 1.0kg/t 32 lyszoyme treatment shown significant redution in microbial diversity. Spirochaetes, Euryarchaeota 33and Actinobacteria significantly increased while Firmicutes showed a remarkable reduction in 34 1.0kg/t treated group compared with control. Pyrimidine metabolism,Purine metabolism and 35Amino acid related enzymes were significantly upregulated in 1.0kg/t lysozyme treated group. 36The richness of gram-positive bacteria were significantly down-regulated by lysozyme 37 treatments.Serum aspartate transaminase (AST) activity was significantly un-regulated.Serum IgM 38 levels were significantly higher in the 1.0 kg/t group compared with control, while IgA levels was 39 significantly lower in 1.0kg/t group.Over thirty metabolites from sow's breast milk including 40 L-Glutamine,creatine and L-Arginine were sigficantly altered by lysozyme treatment.There 41 existed crucial correlations among gut microbiota, serum immunity and breast milk metabolites 42where lactobacillus and prevotella may play a key role in lysozyme mediated host-microbial 43 interactions. Overall, lysozyme supplementation could effectively improve the composition, 44 metabolic functions and phenotypes of sow's gut microbiota and it also benefit sows with better 45 immune status and breast milk composition. 46 47 Importance: Enteric infections caused by pathogens have a significant negative effect on neonatal 48 survival and animal health in swine production. The application of antibiotics in feeds at 49 subtherapeutic levels could improve performance and overall health and is used extensively 50 throughout the industry. However, abuse of antibiotics is contributing to the high level of drug 51 resistance in microbial communities and rising concerns regarding human health. Here, we 52 revealed that lysozyme supplementation could effectively improve the composition, metabolic 53 functions and phenotypes of sow's gut microbiota and it also benefit sows with better immune 54 status and breast milk composition. These findings confirmed that lysozyme could be a suitable 55 alternative to antibiotics in swine production. 56 57 58 59 60 lysozyme alters sow's gut microbiota and health 61 62 63 64 65 66 67

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Postnatal growth retardation is associated with deteriorated intestinal mucosal barrier function using a porcine model

Tan

Wang

et al. 2020

Journal Cellular Physiology

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Individuals with postnatal growth retardation (PGR) are prone to developing chronic diseases. Abnormal development in small intestine is casually implicated in impaired growth. However, the exact mechanism is still implausible. In this present study, PGR piglets (aged 42 days) were employed as a good model to analyze developmental changes in intestinal mucosal barrier function. Our data demonstrated that PGR piglets exhibited impaired jejunal and ileal epithelial villous morphology and permeability, accompanied by decreased cell proliferation ability and increased apoptosis rate. In addition, the expression of tight junction proteins (ZO‐1, claudin 1, and occludin) and E‐cadherin was markedly inhibited by PGR. The expression of P‐glycoprotein was significantly reduced in PGR piglets, as well as decreased activity of lysozyme. Moreover, the mRNA abundance and content of inflammatory cytokines were significantly increased in the intestinal mucosa and plasma of PGR piglets, respectively. PGR also contributed to lower level of sIgA, and higher level of CD68‐positive rate, β‐defensins, and protein expression involved p38 MAPK/NF‐κB pathway. Furthermore, PGR altered the intestinal microbial community such as decreased genus Alloprevotella and Oscillospira abundances, and led to lower microbial‐derived butyrate production, which may be potential targets for treatment. Collectively, our findings indicated that the intestinal mucosal barrier function of PGR piglets could develop the nutritional intervention strategies in prevention and treatment of the intestinal mucosal barrier dysfunction in piglets and humans.

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Effect of lysozyme or antibiotics on faecal zoonotic pathogens in nursery pigs

Abstract: Lysozyme can effectively replace antibiotics in the diet of nursery swine and can be effective for pathogen control.

Cited by 23 publications

References 43 publications

Effects of dietary lysozyme levels on growth performance, intestinal morphology, immunity response and microbiota community of growing pigs

Effects of dietary lysozyme levels on growth performance, intestinal morphology, immunity response and microbiota community of growing pigs

Dietary lysozyme supplement alters serum biochemical makers and milk metabolite profile of sows via gut microbiota

Postnatal growth retardation is associated with deteriorated intestinal mucosal barrier function using a porcine model

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