Effect of Lutein on Cytochrome P450 (Isoform CYP3A4) - An in vitro Study

Meenapriya, Murthy; Anitha, R.; Lakshmi, T

doi:10.5530/pj.2018.6.185

Cited by 4 publications

(7 citation statements)

References 12 publications

(12 reference statements)

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“…Prathapan et al used the same method for studying the effect of lutein on differentiation and the cells were incubated at different concentrations later quantified using multiple plate readers at 490 nm. This preparation was similar to the preparation used in the present study [17,18]. The leaves of coriander show stronger antioxidant activity than seeds, but in both the parts of coriander, ethyl acetate extract showed the strongest ability.…”

Section: Discussionsupporting

confidence: 60%

Cytotoxic Effect of Coriander Oleoresin against Lung Cancer Cell Line A549

Tamilmani

Anitha

Subramanian

et al. 2021

JPRI

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Aim: The main aim of present study was to assess the cytotoxic effect of coriander oleoresin against lung cancer cell line A549. Introduction: Coriandrum sativum (Coriander), family Umbelliferae. Coriander contains mainly essential oil and has antioxidant, diuretic, anti-diabetic anticonvulsant, hypnotic sedative, anti-mutagenic, antimicrobial, anthelmintic activity. Materials and Methods: Coriander oleoresin (product number: 4010000243) was obtained from Synthite Industries Private Limited, Kerala. In the present study, lung cancer cell line A549 was treated with coriander oleoresin at different concentrations and later evaluated for its cytotoxic activity using MTT assay. Results: The cytotoxic effect of coriander oleoresin on lung cancer cell line was proved; the drug concentration increased, the percentage of cell viability decreased proving its cytotoxic effect. The coriander oleoresin has shown a dose dependent cytotoxic effect on lung cancer cell lines. As the drug concentration increased, the percentage of cell viability decreased proving its cytotoxic effect. The IC50 value was 80 μg/ml. Conclusion: In the present study, coriander oleoresin showed a good cytotoxic effect on lung cancer cell lines which may be helpful in treatment of lung cancer. However more research is needed to understand the underlying mechanisms of the cytotoxicity property of the plants.

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Section: Discussionsupporting

confidence: 60%

Cytotoxic Effect of Coriander Oleoresin against Lung Cancer Cell Line A549

Tamilmani

Anitha

Subramanian

et al. 2021

JPRI

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“…Of specific interest in this context is the CYP3A4 which is an important enzyme in the metabolism of drugs and several other xenobiotics. Interactions of xenobiotics including drugs with CYP3A4 have been demonstrated to either be inhibitory or inductive (Meenapriya et al., 2018), and such effect could influence the pharmacokinetics of any co‐administered agent. In this study, the absence of inhibitory effect of GLH on CYP3A4 could suggest that it is not likely to have inhibitory DDI with drugs currently metabolized by CYP3A4 isoform.…”

Section: Discussionmentioning

confidence: 99%

“…They have also been identified as major detoxification system in drug metabolism processes. Alterations in drug metabolism facilitated by other co‐administered agents or drugs could potentiate metabolic and/or systemic drug–drug interaction (DDI) (Meenapriya et al., 2018). Unpredicted DDIs have been implicated in severe adverse effects or treatment failures and usually involve either the induction or inhibition of specific CYP activity (Asha & Vidyavathi, 2010; Clarke & Jones, 2008; Meenapriya et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Alterations in drug metabolism facilitated by other co‐administered agents or drugs could potentiate metabolic and/or systemic drug–drug interaction (DDI) (Meenapriya et al., 2018). Unpredicted DDIs have been implicated in severe adverse effects or treatment failures and usually involve either the induction or inhibition of specific CYP activity (Asha & Vidyavathi, 2010; Clarke & Jones, 2008; Meenapriya et al., 2018). Unlike in most instances where biotransformation results in metabolic activation of a prodrug, metabolic inactivation is usually characterized by either inhibition that could aggravate drug toxicity resulting from exposure to a therapeutic agent, or induction, which could culminate in loss of efficacy attributable to reduction in drug exposure (Gurley, 2012).…”

Section: Introductionmentioning

confidence: 99%

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An insight on the nature of biochemical interactions between glycyrrhizin, myricetin and CYP3A4 isoform

Sabiu

Idowu

2021

Journal of Food Biochemistry

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Drug interaction studies are imperative to gain insights into the beneficial or harmful effects of therapeutic and dietary agents. This study investigated the mechanism of modulatory roles of glycyrrhizin (GLH) and myricetin (MYC) on the human CYP3A4 isoform using in silico and in vitro methods. While MYC had concentration‐dependent inhibitory effect on CYP3A4 (IC50: 10.5 ± 0.55 μM) with characteristic Km and Vmax values of 1.13 μM and 1.54 nM/min, respectively, GLH exhibited no inhibitory effect on CYP3A4 activity in vitro. These observations are consistent with the results of in silico evaluations where the effect of MYC compared well with that of ketoconazole (a known CYP3A4 inhibitor) against CYP3A4. Overall, the established interactions between the study compounds and CYP3A4 could potentiate clinically vital drug–drug interactions and has lent credence to the mechanism of modulatory effect of MYC and GLH on CYP3A4 that could guide their safe use as therapeutic agents. Practical implications Myricetin (MYR) and glycyrrhizin (GLH) occur freely in commonly ingested foods and their supplements are recommended for the treatment of several debilitating diseases such as diabetes, cancer, and cardiovascular complications. This study provided an insight on the possible interactions that could be established between these compounds (MYR and GLH) and CYP3A4 when ingested and metabolized by the liver. The results suggested possibilities of potential clinical drug–drug interactions and advocates for their cautious use within the therapeutic dose in food supplements or medications to avoid probable liver damage.

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“…Since human cancer cell lines maintain hallmark characteristics of cancer cells, are easy to propagate, and can be genetically engineered to provide reproducible This procedure may also differentiate between cells in various stages of apoptosis. As a result, AO/EtBr staining may be used in DSTs [31]. The morphological changes in cells treated with Lippia nodiflora leaf extract were also detected, and the findings were significant.…”

Section: Inverted Phase Contrast Microscope Was Used To Examine the C...mentioning

confidence: 92%

Pro-Apoptotic Effect of Lippia nodiflora Leaf Extract against the Skin Cancer Cell Line

Thariny

Elumalai

Subramanian

et al. 2021

JPRI

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Background: Skin cancers are cancers that develop on the surface of the skin. They occur as a result of the formation of irregular cells with the potential to infiltrate or migrate to other areas of the body. Natural medicinal resources, such as the traditional herbal remedy Lippia nodiflora, have long been used to treat dermatological disorders including skin inflammation and melanogenesis. Apoptosis serves as a promising pathway in controlling cancer. However there are minimal amounts of studies exploring its proapoptotic activity of Lippia nodiflora in skin cancer cells. Aim: The aim of this present study was to evaluate the pro-apoptotic effect of Lippia nodiflora leaf extract against the skin cancer cell line. Materials and Methods: MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays were used to determine the cytotoxic effect of Lippia nodiflora leaf extract against skin cancer cells (B16-F10). The skin cancer cells were treated with different concentration Lippia nodiflora leaf extract for 24h. We calculated the optimum (IC-50; 20µg/ml) using the MTT assay, which was then used for further analysis. A phase-contrast microscope is used to analyse changes in cell morphology. AO/EtBr dual staining under a fluorescence microscope was used to determine the effect of Lippia nodiflora on B16-F10 cell death. Results: In our research, the cell viability of the B16-F10 skin cancer cell line was dramatically decreased after treatment with different concentrations (5-60 µg/ml) of Lippia nodiflora for 24 hours. 50% inhibition was detected at a concentration of 20 µg/ml, which was determined to be an inhibitory concentration (IC-50) value and used in subsequent studies. The viable cell will possess a uniform bright green nucleus. Early apoptotic cells will have bright orange areas of condensed or fragmented chromatin in the nucleus. Late apoptotic cells will have uniform bright red nucleus. Conclusion: The findings of this analysis revealed that Lippia nodiflora leaf extract inhibits the cell viability and indces apoptosis of skin cancer cells. Thus, Lippia nodiflora exhibits anti-cancer effects against skin cancer cells, therefore it raises new hope for anti-cancer therapy.

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Effect of Lutein on Cytochrome P450 (Isoform CYP3A4) - An in vitro Study

Cited by 4 publications

References 12 publications

Cytotoxic Effect of Coriander Oleoresin against Lung Cancer Cell Line A549

Cytotoxic Effect of Coriander Oleoresin against Lung Cancer Cell Line A549

An insight on the nature of biochemical interactions between glycyrrhizin, myricetin and CYP3A4 isoform

Pro-Apoptotic Effect of Lippia nodiflora Leaf Extract against the Skin Cancer Cell Line

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