Effect of &lt;i&gt;Punica granatum&lt;/i&gt; and &lt;i&gt;Tecomella undulata&lt;/i&gt; supplementation on nutrient utilization, enteric methane emission and growth performance of Murrah male buffaloes

Hundal, J.S.; Singh, I. S.; Wadhwa, M.; Singh, C. C.; Uppal, C.; Kaur, Gagandeep

doi:10.22358/jafs/109237/2019

Cited by 15 publications

(7 citation statements)

References 46 publications

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“…The significant reduction in methane witnessed with eragrostis hay could be attributed to the antimethanogenic properties of the PSMs present in the extracts, as previously noted by Akanmu and Hassen [2]. The interaction of different PSMs present in the same medicinal plant, with the diverse microbial population in the rumen might yield different results from the use of pure compounds, such as purified tannins or saponins [29], especially in terms of its methane mitigation potential. In the study by Singh et al [30], aqueous extract blends from Sapindus mukorossi, Ficus bengalensis and Eucalyptus essential oil reduced methane concentration with increasing inclusion of plant extract blends in both oat hay and TMR substrate.…”

Section: Resultsmentioning

confidence: 57%

Gas Production, Digestibility and Efficacy of Stored or Fresh Plant Extracts to Reduce Methane Production on Different Substrates

Akanmu

Hassen

Adejoro

2020

Animals

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Natural compounds such as plant secondary metabolites (PSM) can be used to replace antibiotic growth promoters as rumen modifiers. In this study, the effectiveness of stored and freshly extracted Aloe vera (AV), Azadirachta indica (AZ), Moringa oleifera (MO), Jatropha curcas (JA), Tithonia diversifolia (TD) and Carica papaya (CP) crude extract and monensin on in vitro gas and methane production, organic matter digestibility (IVOMD) and volatile fatty acids (VFA) were evaluated using a total mixed ration (TMR), lucerne or Eragrostis curvula substrates. Fresh extracts were processed from the same batch of frozen (−20 °C) plant material a few days before the trial while the stored extracts were extracted and stored at 4 °C for 12 months prior to the study. Extraction was done by solubilising 50 g freeze-dried plant material in 500 mL 100% methanol. Four mL of reconstituted 50 mg crude extract per 1000 mL distilled water was added per incubation vial, which already contained 400 mg substrate and in vitro fermentation, and gas production and IVOMD evaluation were carried out using standard procedures. Results showed that storing plant extracts for 12 months did not affect the activity or stability of metabolites present in the crude extracts, as shown by the lack of differences in total gas production (TGP) and methane produced between fresh or stored extracts across the substrates. In the TMR substrate, plant extracts increased IVOMD but did not affect TGP and methane production, whereas monensin did not have any effect. Plant extracts increased IVOMD of Eragrostis substrate and supressed methane production to a greater extent than monensin (p < 0.05). It can be concluded that storing plant extracts for up to 12 months did not compromise their efficacy. In addition, the use of 50 mg/kg of AV, AZ, MO, JA, TD and CP extract to a forage-based diet will reduce methane production while improving feed digestibility.

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Section: Resultsmentioning

confidence: 57%

Gas Production, Digestibility and Efficacy of Stored or Fresh Plant Extracts to Reduce Methane Production on Different Substrates

Akanmu

Hassen

Adejoro

2020

Animals

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“…To this end, there are used probiotic microorganisms that have a beneficial effect on the digestibility of nutrients that provide protection against pathogenic microflora or populate free niches in this microbiome. In addition, plant extracts [8,9] or other molecular compositions may be used to improve biomass growth. A way to assess the metabolic state of bacteria of rumen microflora cells is the registration of glow kinetics including recombinant strains, which is a reflection of stress states developing in the cell.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of effects of rumen fluid in combination with probiotic preparations and vanillin on the luminescence of a recombinant strain E. coli

et al. 2020

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A way to assess the metabolic state of bacteria of rumen microflora cells is the registration of glow kinetics including recombinant strains, which is a reflection of stress states developing in the cell. The experiment evaluated the effect of rumen fluid in combination with probiotic strains and vanillin on the luminescence of a recombinant strain Escherichia coli MG1655 and digestibility of feed. The “in vitro” method with the rumen simulation technique (Rusitec) was used to determine the digestibility of the dry substance of feed. Using the initial sample, the glow level at the 30th minute was recorded at 3,149±145 RLU, which is 32% of the control level. In the presence of rumen fluid (at the concentration of 6.25%), there was observed an improved response by E.coli against Saccharomyces boulardii’s background (by 44%). The use of vanillin in rumen fluid increases the glow level of bacterial cells in the range of concentrations up to 50%, and in combination with probiotic strains, in the range of 25-50 %. In vitro conditions against the background of rumen fluid showed that vanillin in combination with probiotic strains Bifidobacterium longum and Bifidobacterium adolescentis / Lactobacillus acidophilus contributes to an increase in the digestibility of the dry substance of feed.

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“…The positive or negative effects of tannin on CH 4 production may vary depending on the amount of tannin. The condensed tannins reduce methane production from ruminants either indirectly through a reduction in fibre digestion, which decreases H 2 production or directly through inhibition of the growth of methanogens (Hundal et al 2019a).…”

Section: Chemical Composition Of Different Parts Of Colocynth and Sub...mentioning

confidence: 99%

“…But effects of plant secondary metabolites (tannins, saponins, essential oils, flavonoids etc.) on methanogenesis and rumen function are variable and source dependent , Hundal et al 2019a. Therefore, there is need to explore new herbal feed additives (HFA) to assess their potential.…”

mentioning

confidence: 99%

Potential of Citrullus colocynthis as herbal feed additive for ruminants

Hundal

Wadhwa

Singh

et al. 2020

Indian J of Anim Sci

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To assess the potential of Citrullus colocynthis as herbal feed additive for ruminants, colocynth fruit as a whole, its seeds and peel-pulp were evaluated at various levels (0, 0.5, 1.0, 1.5, 2.0, 2.5, 4.0%) with total mixed ration (roughage to concentrate ratio: 65:35) as substrate in 3×7 factorial design by in vitro gas production technique. Phytochemical analysis revealed significant variation (P<0.01) in total phenols, non-tannin phenols, true tannins, saponins, flavonoids and vitamin C content among different parts of colocynth fruit. Irrespective of the part used, varying level of Citrullus colocynthis had significant effect on net gas production (NGP; ml/24/g), metabolizable energy (ME) availability, methanogenesis, digestibility of neutral detergent fiber (NDF) and true organic matter (TOM) in comparison to control; however the effect was pronounced at 1% level of supplementation. No significant difference in total volatile fatty acids (TVFA) production and acetate production was observed at varying levels of Citrullus colocynthis except at 4% level when total mixed ration (TMR) was used as substrate. Irrespective of its level, supplementation of Citrullus colocynthis fruit as a whole, seeds and peel-pulp significantly improved NGP (P<0.001) and ME (P<0.01) availability in comparison to control group whereas partitioning factor (PF; P>0.578), digestibility of NDF (P>0.905) and true OM (P>0.228) remained unaffected between different test groups. However, TVFA production, acetate, propionate and butyrate concentration was observed lower (P<0.001) in peel-pulp and seed supplemented group. From above results it can be concluded that supplementation of Citrullus colocynthis fruit at 1% level of substrate had potential to improve rumen fermentation characteristics and reduce methanogenesis, however in vivo assessment on ruminants need to be conducted to evaluate the persistency of effects along with health concerns.

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Effect of <i>Punica granatum</i> and <i>Tecomella undulata</i> supplementation on nutrient utilization, enteric methane emission and growth performance of Murrah male buffaloes

Cited by 15 publications

References 46 publications

Gas Production, Digestibility and Efficacy of Stored or Fresh Plant Extracts to Reduce Methane Production on Different Substrates

Gas Production, Digestibility and Efficacy of Stored or Fresh Plant Extracts to Reduce Methane Production on Different Substrates

Evaluation of effects of rumen fluid in combination with probiotic preparations and vanillin on the luminescence of a recombinant strain E. coli

Potential of Citrullus colocynthis as herbal feed additive for ruminants

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