Effect of <i>Elaeagnus Conferta</i> Roxb (Elaeagnaceae) Dry Fruit on the Activities of Hepatic Alcohol Dehydrogenase and Aldehyde Dehydrogenase in Mice

Abstract

Purposes: To determine the effect of Elaeagnus conferta Roxb dry fruit powder (ECR) on blood alcohol clearance and on the activities of hepatic alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). Methods:In a randomized controlled study, acute alcohol intoxication was induced in mice with Hongxing liquor containing 65 % v/v ethanol orally. The effect of ECR on blood alcohol clearance and on the activities of hepatic alcohol dehydrogenase and aldehyde dehydrogenase in the mice was then investigated. Results: A 30-min pretreatment with ECR at 400 and 800 mg/kg led to a faster clearance of blood alcohol after the alcohol ingestion. The concentration of blood alcohol at 4 h after alcohol ingestion decreased by 21.2 % in mice pretreated with 800 mg/kg ECR. The activities of hepatic alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) were enhanced by ECR. Conclusion: These results suggest that pretreatment with ECR might stimulate the clearance of blood alcohol by increasing the activities of hepatic alcohol dehydrogenase and aldehyde dehydrogenase.

Effects of Beverages on Alcohol Metabolism: Potential Health Benefits and Harmful Impacts

“…ADH activity was determined by the modified method of Wu et al [ 33 ]. In brief, 0.1 mL of 0.3 mol/L ethanol and 2.8 mL 0.0021 mol/L NAD + (prepared by 0.1 mol/L Tirs-HCl buffer, pH 7) were mixed at 25 °C, and then 0.1 mL of the liver supernatant was added to initiate the reaction.…”

Effects of 20 Selected Fruits on Ethanol Metabolism: Potential Health Benefits and Harmful Impacts

“…Perhaps this was because Musa nana has high content of potassium. In the literature, Elaeagnus conferta was reported to increase the activity of ADH, and also had a high ratio of K/Na [ 18 ]. Mangifera indica and Durio zibethinus juices didn’t significantly affect the activities of ADH and ALDH in this study, while it was reported in the literature that Mangifera indica fruit could increase the activities of ADH and ALDH and Durio zibethinus fruit extracts inhibited yeast ALDH activity in vitro [ 12 , 31 ].…”

“…Hepatic ADH and ALDH activities were evaluated according to Wu et al [ 18 , 19 ]. Briefly, the liver tissues were weighed and homogenized at a 1:9 ratio ( w / v ) at 4 °C in 0.1 M Tris-HCl (pH 7.0).…”