Effect of low-temperature stress on the growth of plants of Secale cereale (Poaceae) and endogenous cytokinin content in roots and shoots

Abstract

Phytohormones play a key role in the regulation of plant acclimation to low temperature. To elucidate the role of cytokinins in rye plant response to chilling, we studied the dynamics of these hormones in shoots and roots under short-term and prolonged cold stress. The 7-day-old plants were exposed to cold stress (2 °C) for 2 h (alarm phase of response) or for 6 h for two days (acclimation phase of response). Endogenous content of cytokinins was analyzed by HPLC-MS method. Low temperature had a differential effect on the content of individual cytokinins and their localization in rye plants. During the short-term stress, a decrease in the content of active cytokinins (trans-zeatin and trans-zeatin riboside) in the roots and an increase in the shoots were shown. Prolonged low-temperature stress declined the amount of cytokinins except trans-zeatin riboside, which was detected in both roots and shoots. Significant rise in trans-zeatin riboside content in roots and shoots in this period evidenced an important role of this cytokinin during cold acclimation of rye plants.