Effect of low-temperature plasma treatment of electrospun polycaprolactone fibrous scaffolds on calcium carbonate mineralisation

Ivanova, Anna; Syromotina, Dina Sergeevna; Shkarina, Svetlana; Shkarin, Roman; Cecilia, A.; Weinhardt, Venera; Baumbach, Tilo; Saveleva, Mariia; Gorin, Dmitry A.; Douglas, Timothy E.L.; Parakhonskiy, Bogdan; Skirtach, André G.; Cools, Pieter; Geyter, Nathalie De; Morent, Rino; Oehr, Christian; Surmenev, Roman A.

doi:10.1039/c8ra07386d

Cited by 35 publications

(30 citation statements)

References 49 publications

(81 reference statements)

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“…The use of X-ray tomography together with sophisticated 3D analysis has been demonstrated well in this study as well as recent studies investigating different polycaprolactone scaffolds for bone tissue regeneration [49,50] and after checking the effect of mineralisation behaviour of CaCO 3 deposited on such scaffolds followed by 2 plasma treatment [51]. It is evident that the future will involve using such methods not only for investigating scaffold characteristics but will combine detailed cell analysis investigating cell adhesion, growth, viability morphology and differentiation [51]. Nevertheless, X-ray microCT methodology is costly and involves having access to a synchrotron facility but has advantages requiring no staining, high flux.…”

Section: B Dsupporting

confidence: 57%

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Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays

Bhartiya

Madi²,

Disney

et al. 2020

J Synchrotron Radiat

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Advanced imaging is useful for understanding the three-dimensional (3D) growth of cells. X-ray tomography serves as a powerful noninvasive, nondestructive technique that can fulfill these purposes by providing information about cell growth within 3D platforms. There are a limited number of studies taking advantage of synchrotron X-rays, which provides a large field of view and suitable resolution to image cells within specific biomaterials. In this study, X-ray synchrotron radiation microtomography at Diamond Light Source and advanced image processing were used to investigate cellular infiltration of HeLa cells within poly L-lactide (PLLA) scaffolds. This study demonstrates that synchrotron X-rays using phase contrast is a useful method to understand the 3D growth of cells in PLLA electrospun scaffolds. Two different fiber diameter (2 and 4 µm) scaffolds with different pore sizes, grown over 2, 5 and 8 days in vitro, were examined for infiltration and cell connectivity. After performing visualization by segmentation of the cells from the fibers, the results clearly show deeper cell growth and higher cellular interconnectivity in the 4 µm fiber diameter scaffold. This indicates the potential for using such 3D technology to study cell–scaffold interactions for future medical use.

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Section: B Dsupporting

confidence: 57%

“…This study will allow us to identify suitable platforms for cancer cell biology and metastasis, in which we will be able to understand the complicated behavior of cells. Imaging the interior of scaffolds will also serve useful for other applications such as tissue regeneration and stem cell generation [51].…”

Section: Resultsmentioning

confidence: 99%

Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays

Bhartiya

Madi²,

Disney

et al. 2020

J Synchrotron Radiat

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“…Plasma treatment is one of the most common and effective ways of post-functionalisation to promote hydrophilicity of the polymer surfaces by adding polar groups to the surface of the material without altering the bulk properties ( Jokinen et al, 2012 ; Abedalwafa et al, 2013 ; Valence et al, 2013 ; Shafei et al, 2017 ; Ivanova et al, 2018 ). Owen et al showed that both air and acrylic acid plasma treatment improved the attachment and proliferation of mesenchymal progenitors on acrylate-based PolyHIPEs, whereas untreated scaffolds did not support cell attachment ( Owen et al, 2015 , 2016 ).…”

Section: Development Of the Emulsion Templated Scaffoldsmentioning

confidence: 99%

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Dikici

Claeyssens

2020

Front. Bioeng. Biotechnol.

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Tissue engineering (TE) aims to regenerate critical size defects, which cannot heal naturally, by using highly porous matrices called TE scaffolds made of biocompatible and biodegradable materials. There are various manufacturing techniques commonly used to fabricate TE scaffolds. However, in most cases, they do not provide materials with a highly interconnected pore design. Thus, emulsion templating is a promising and convenient route for the fabrication of matrices with up to 99% porosity and high interconnectivity. These matrices have been used for various application areas for decades. Although this polymer structuring technique is older than TE itself, the use of polymerised internal phase emulsions (PolyHIPEs) in TE is relatively new compared to other scaffold manufacturing techniques. It is likely because it requires a multidisciplinary background including materials science, chemistry and TE although producing emulsion templated scaffolds is practically simple. To date, a number of excellent reviews on emulsion templating have been published by the pioneers in this field in order to explain the chemistry behind this technique and potential areas of use of the emulsion templated structures. This particular review focusses on the key points of how emulsion templated scaffolds can be fabricated for different TE applications. Accordingly, we first explain the basics of emulsion templating and characteristics of PolyHIPE scaffolds. Then, we discuss the role of each ingredient in the emulsion and the impact of the compositional changes and process conditions on the characteristics of PolyHIPEs. Afterward, current fabrication methods of biocompatible PolyHIPE scaffolds and polymerisation routes are detailed, and the functionalisation strategies that can be used to improve the biological activity of PolyHIPE scaffolds are discussed. Finally, the applications of PolyHIPEs on soft and hard TE as well as in vitro models and drug delivery in the literature are summarised.

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“…Different working gases such as air, oxygen (O 2 ), nitrogen (N 2 ), ammonium (NH 3 ), argon (Ar), or helium (He) have been used for this purpose [29,[43][44][45][46][47][48]. Most of studies concerning plasma activation of electrospun scaffolds have focused on PCL [29,46,47,[49][50][51] and PLLA [52][53][54][55][56][57][58] while those concerning PLGA [48,[59][60][61][62] are few and still neglected in the literature although its wide application range in the field of tissue engineering [63]. Despite the known cytocompatibility of PLGA [10,11,13,25,64], its poor hydrophilic properties and the rather low ability to interact with cells restrict the natural cell recognition sites on its surface, which may lead to poor overall cell adhesion [65].…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential

et al. 2020

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Electrospun PLGA microfibers with adequate intrinsic physical features (fiber alignment and diameter) have been shown to boost teno-differentiation and may represent a promising solution for tendon tissue engineering. However, the hydrophobic properties of PLGA may be adjusted through specific treatments to improve cell biodisponibility. In this study, electrospun PLGA with highly aligned microfibers were cold atmospheric plasma (CAP)-treated by varying the treatment exposure time (30, 60, and 90 s) and the working distance (1.3 and 1.7 cm) and characterized by their physicochemical, mechanical and bioactive properties on ovine amniotic epithelial cells (oAECs). CAP improved the hydrophilic properties of the treated materials due to the incorporation of new oxygen polar functionalities on the microfibers’ surface especially when increasing treatment exposure time and lowering working distance. The mechanical properties, though, were affected by the treatment exposure time where the optimum performance was obtained after 60 s. Furthermore, CAP treatment did not alter oAECs’ biocompatibility and improved cell adhesion and infiltration onto the microfibers especially those treated from a distance of 1.3 cm. Moreover, teno-inductive potential of highly aligned PLGA electrospun microfibers was maintained. Indeed, cells cultured onto the untreated and CAP treated microfibers differentiated towards the tenogenic lineage expressing tenomodulin, a mature tendon marker, in their cytoplasm. In conclusion, CAP treatment on PLGA microfibers conducted at 1.3 cm working distance represent the optimum conditions to activate PLGA surface by improving their hydrophilicity and cell bio-responsiveness. Since for tendon tissue engineering purposes, both high cell adhesion and mechanical parameters are crucial, PLGA treated for 60 s at 1.3 cm was identified as the optimal construct.

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Effect of low-temperature plasma treatment of electrospun polycaprolactone fibrous scaffolds on calcium carbonate mineralisation

Abstract: This article reports on a study of the mineralisation behaviour of CaCO3 deposited on electrospun poly(ε-caprolactone) (PCL) scaffolds preliminarily treated with low-temperature plasma.

Cited by 35 publications

References 49 publications

Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays

Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential

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