Effect of Low-Power Laser (LPL) and Light-Emitting Diode (LED) on Inflammatory Response in Burn Wound Healing

Abstract

The aim of the study was to investigate the biochemical and molecular changes in the process of epidermal healing of burn injuries after therapeutic treatment with low-power laser (LPL) and light-emitting diode (LED). Rats were divided into six groups: skin without injury (Sham), burn wounds (BWs), BW + 660-nm LPL, BW + 904-nm LPL, BW + 632-nm LED, and BW + 850-nm LED. The burn wound model was performed using a 100 °C copper plate, with 10 s of contact in the skin. The irradiations started 24 h after the lesion and were performed daily for 7 days. The burn wound groups showed an increase in the superoxide production, dichlorofluorescein, nitrites, and high protein oxidative damage. The activities of glutathione peroxidase and catalase were also increased, and a significant reduction in glutathione levels was observed compared to the control group. However, treatments with 660-nm LPL and 850-nm LED promoted protection against to oxidative stress, and similar results were also observed in the IL-6 and pERK1/2 expression. Taken together, these results suggest that LPL 660 nm and LED 850 nm appear reduced in the inflammatory response and oxidative stress parameters, thus decreasing dermal necrosis and increasing granulation tissue formation, in fact accelerating the repair of burn wounds.

Burn Wound Successfully Treated with 830-nm Light Emitting Diode Phototherapy Combined with Epidermal Growth Factor Solution

“…2 Tissue damage in burn wound triggers a cascade of biochemical and molecular events that initiate a rapid but highly complex tissue repair process. 5 Recent study revealed the positive therapeutic effect of treating burn wounds with LED device and EGF solution 1,4 , but there is lack of study with synergistic effect of modalities mentioned above.…”

“…LED can reduce the inflammatory response by blocking the effects of ROS via activation of IL-6 and inhibition of cell proliferation and differentiation induced by growth factors via the MAPK pathway in burn wound model. 4 Application of EGF-containing formulations around lesions is widely used in wound treatments. Sen et al 1 reported applying EGF solution showed faster granulation, enhanced collagenization, patterned re-epithelialization and immunomodulatory effect in diabetic burn wound model.…”

“…2,3 Several studies have reported the development of increasingly efficient approaches for tissue repair, with minimization of the factors that delay or impede the healing process in burn wounds which including applicating epidermal growth factor (EGF)-containing formulations in and/or around lesions, and light-emitting diode (LED) therapy. 1,4 Because of situations mentioned above, many clinicians have a tendency to hesitate treating with single modality in burn wound with various medical conditions. When deciding combination treatment in burn wound, clinicians should consider the synergistic effects between each modalities.…”

Topical treatment with a transient receptor potential ankyrin 1 (TRPA1) antagonist reduced nociception and inflammation in a thermal lesion model in rats

“…Thus, many studies have demonstrated that the hydrogen peroxide production by tissue-resident cells (i.e., synoviocytes), macrophages and neutrophils, could favor the sensitization of nociceptors via TRPA1 activation (Moilanen et al, 2015;Trevisan et al, 2016Trevisan et al, , 2014. Previous studies reported that thermal burn might increase the reactive species production, which could contribute to the tissue damage (Bhatia et al, 2016;Hoşnuter et al, 2004;Parihar et al, 2008;Silveira et al, 2016;Tanaka et al, 1999). In this sense, antioxidants are designed to reduce production and to capture reactive species that promote tissue lesion (Wasiak et al, 2013).…”

Infrared Skin‐Like Active Stretchable Electronics Based on Organic–Inorganic Composite Structures for Promotion of Cutaneous Wound Healing

“…There are many effective therapeutic methods for diminishing inflammation, reducing swelling, relieving pain, improving microcirculation, and promoting wound healing, e.g., photodynamic, microcurrent, and ultrasonic treatments, among which the photodynamic therapy has been most widely used due to its noninvasive character and outstanding therapeutic effects. Specifically, the near infrared (NIR) light, used as a low‐level laser therapy (LLLT), has proven effective in controlling pain and reducing the healing time by stimulating collagen production, fibroblast proliferation, and the local microvasculature as well as by stimulating cellular metabolism to increase the potential for tissue regeneration . As a light source of NIR, the light emitting diodes (LEDs) have been recently used for medical treatments, showing great benefits to wound healing .…”