Effect of low pH and salinity conditions on the antioxidant response and hepatocyte damage in juvenile olive flounder Paralichthys olivaceus

Lee, Dae-Won; Choi, Young-Ung; Park, Heung-Sik; Park, Young-Su; Choi, Cheol Young

doi:10.1016/j.marenvres.2022.105562

Cited by 13 publications

(5 citation statements)

References 32 publications

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“…Recent studies have analyzed the combined effects of global warming and ocean acidification on various marine organisms [16,17] and found that their combined effects exert stronger impacts on the physiology of shellfish than global warming alone [18,19]. Therefore, it is crucial to investigate the physiological responses of marine organisms to the combined effects of these factors to comprehend their impacts.…”

Section: Introductionmentioning

confidence: 99%

Oxidative Stress and Apoptosis in Disk Abalone (Haliotis discus hannai) Caused by Water Temperature and pH Changes

Kim

Lee

et al. 2023

Antioxidants

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Ocean warming and acidification can induce oxidative stress in marine species, resulting in cellular damage and apoptosis. However, the effects of pH and water temperature conditions on oxidative stress and apoptosis in disk abalone are poorly understood. This study investigated, for the first time, the effects of different water temperatures (15, 20, and 25 °C) and pH levels (7.5 and 8.1) on oxidative stress and apoptosis in disk abalone by estimating levels of H2O2, malondialdehyde (MDA), dismutase (SOD), catalase (CAT), and the apoptosis-related gene caspase-3. We also visually confirmed apoptotic effects of different water temperatures and pH levels via in situ hybridization and terminal deoxynucleotidyl transferase dUTP nick end labeling assays. The levels of H2O2, MDA, SOD, CAT, and caspase-3 increased under low/high water temperature and/or low pH conditions. Expression of the genes was high under high temperature and low pH conditions. Additionally, the apoptotic rate was high under high temperatures and low pH conditions. These results indicate that changes in water temperature and pH conditions individually and in combination trigger oxidative stress in abalone, which can induce cell death. Specifically, high temperatures induce apoptosis by increasing the expression of the apoptosis-related gene caspase-3.

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Section: Introductionmentioning

confidence: 99%

Oxidative Stress and Apoptosis in Disk Abalone (Haliotis discus hannai) Caused by Water Temperature and pH Changes

Kim

Lee

et al. 2023

Antioxidants

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“…Fishing location had the greatest influence on elevated oxidative damage, demonstrated by Baltic Sea fish exhibiting significantly higher oxidative damage than North Sea fish. Changes in salinity can cause oxidative stress in fish [54] and a study on olive flounder ( Paralichthys olivaceus ) found that hyposaline conditions caused oxidative stress [55] so it is possible that the low saline Baltic environment is increasing oxidative damage in flounders and dabs. Additionally, a report by the European Environment Agency suggests a larger proportion of the Baltic (96%) is polluted than the North Sea area (75%) [56] which could cause increased oxidative damage in the Baltic.…”

Section: Discussionmentioning

confidence: 99%

Correlations between oxidative DNA damage and formation of hepatic tumours in two flatfish species from contaminated environments

et al. 2023

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Many species in aquatic environments face increased exposure to oncogenic pollution due to anthropogenic environmental change which can lead to higher cancer prevalence. The mechanistic relationship connecting environmental pollution and cancer is multi-factorial and poorly understood, and the specific mechanisms are so far still uncharacterized. One potential mediator between pollutant exposure and cancer is oxidative damage to DNA. We conducted a study in the field with two flatfish species, European flounder ( Platichthys flesus L.) and common dab ( Limanda limanda L. ) with overlapping distribution and similar ecological niche, to investigate if the link between oncogenic pollutants and cancer described in ecotoxicological literature could be mediated by oxidative DNA damage. This was not the case for flounders as neither polycyclic aromatic hydrocarbon (PAH) bile metabolites nor metallic trace element concentrations were related to oxidative DNA damage measurements. However, dabs with higher PAH concentrations did exhibit increased oxidative damage. High oxidative DNA damage also did not predict neoplasm occurrence, rather, healthy individuals tended to have higher oxidative damage measurements compared to fishes with pre-neoplastic tumours. Our analyses showed that flounders had lower concentrations of PAH bile metabolites, suggesting that compared to dab this species is less exposed or better at eliminating these contaminants.

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“…Salinity stress may induce the proapoptotic caspase system (including Casp-9 up-stream and Casp-3 downstream) which executes apoptosis in oliver flounder ( Lee et al, 2022 ). The apoptotic pathways are mediated by caspases, and as such, cells dying without the participation of caspases do not display the typical morphological characteristics of apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Differential Branchial Response of Low Salinity Challenge Induced Prolactin in Active and Passive Coping Style Olive Flounder

Zeng

Yang

et al. 2022

Front. Physiol.

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Stress coping styles are very common in fish, and investigations into this area can greatly improve fish welfare and promote the sustainable development of aquaculture. Although most studies have focused on the behavioral and physiological differences of these fishes, the endocrine response of different coping styles fish when undergoing salinity challenge is still unclear. We examined the physiological response in olive flounder with active coping (AC) style and passive coping (PC) style after transferred from seawater (SW) to freshwater for 0, 2, 5, 8, and 14 days. The results showed that: 1) the plasma prolactin level of FW-acclimated AC flounder was substantially higher than that of FW-acclimated PC flounder at 5, 8, and 14 days, and the branchial gene expression of prolactin receptor (PRLR) in AC flounder was slightly higher than PC flounder after transfer. While there was no remarkable difference observed in cortisol (COR) levels between AC and PC flounder. After transfer, glucocorticoid receptor (GR) expression in AC flounder was significantly higher compared with PC flounder at 8 days. 2) Branchial NKA-IR ionocytes numbers were reduced in PC flounder after transfer, while ionocytes number remain stable in AC flounder. 3) The branchial stem cell transcription factor foxi1 gene expression of AC flounder was significantly higher than PC flounder at 2, 5, and 14 days after transfer, while branchial stem cell transcription factor p63 gene expression of FW-acclimated AC flounder was only substantially higher than that of PC flounder at 5 days. 4) As an apoptosis upstream initiator, the branchial gene expression of caspase-9 in PC flounder was considerably higher than in AC flounder after transfer at 8 days. This study revealed that olive flounder with active and passive coping styles have different endocrine coping strategies after facing the low-salinity challenge. AC flounder adopt an active endocrine strategy by increasing ionocyte differentiation and prolactin secretion significantly. In contrast, PC flounder employ a passive strategy of reducing ionocytes differentiation and retaining prolactin content at a low level to reduce branchial ionocytes number.

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Effect of low pH and salinity conditions on the antioxidant response and hepatocyte damage in juvenile olive flounder Paralichthys olivaceus

Cited by 13 publications

References 32 publications

Oxidative Stress and Apoptosis in Disk Abalone (Haliotis discus hannai) Caused by Water Temperature and pH Changes

Oxidative Stress and Apoptosis in Disk Abalone (Haliotis discus hannai) Caused by Water Temperature and pH Changes

Correlations between oxidative DNA damage and formation of hepatic tumours in two flatfish species from contaminated environments

Differential Branchial Response of Low Salinity Challenge Induced Prolactin in Active and Passive Coping Style Olive Flounder

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