Effect of low molecular weight heparin on fracture healing in a stabilized rat femur fracture model

Abstract

The purpose of this study was to evaluate the effect of low molecular weight heparin (LMWH) on fracture healing in a standard stabilized rat femur fracture model. A closed, middiaphyseal transverse fracture was created in the right femur of Long-Evans rats after insertion of a 0.8-mm K-wire into the medullary canal. Animals were randomized to receive either LMWH (70 units/kg dalteparin) or an injection of normal saline daily for 2 weeks. Animals were sacrificed at 2, 3, and 6 weeks. Fracture healing was assessed by radiographs, histology, and mechanical testing. There were no significant differences between the control and LMWH groups in the percentage of animals with radiographic bridging callus at each time point. Histologic appearance of fracture healing was similar between the control and LMWH groups. There were no significant differences in the normalized mechanical properties of the control and LMWH groups at 2 and 3 weeks. At 6 weeks, the percent torque of the LMWH group was significantly greater than the control group ( p ¼ 0.0072), however, there was no significant difference in the stiffness and energy absorption. Dalteparin, at the dosage used in this study, did not impair fracture healing in this standard stabilized rat femur fracture model. ß

Fondapari̇nuksun Kirik İyi̇leşmesi̇ne Gerçekten Pozi̇ti̇f Etki̇si̇ Var Midir? Siçanlar Üzeri̇nde Yapilan Deneysel Bi̇r Çalişma

“…In our study we found that enoxaparin has no negative effects both radiologically and histologically. This result is similar with previous studies (8,11,27). We think that our study follow up is for 4 weeks, which is average for fracture healing process in rats.…”

“…But according to some publications LMWH have negative effects on fracture healing as heparin (8,26,27). There some papers regarding the LMWH have no negative effects on fracture healing (9,27).…”

“…But according to some publications LMWH have negative effects on fracture healing as heparin (8,26,27). There some papers regarding the LMWH have no negative effects on fracture healing (9,27). Also there are some papers concluded that the LMWH can effect bone turnover and healing process negatively when they used for a long time period with high doses (28)(29)(30).…”

Antioxidant Therapy Attenuates Deficient Bone Fracture Repair Associated With Binge Alcohol Exposure

“…Limitations of the current study includes use of a rodent model of alcohol exposure and traumatic orthopaedic injury, however, this model has been utilized in previous studies of traumatic orthopaedic injury 24, 33, 34 . Because a blunt trauma device was used to create the injury, it was impossible to ensure exact reproducibility in fracture site and orientation; some fractures were slightly oblique or more distal than anticipated.…”

The influence of low molecular weight heparin on the expression of osteogenic growth factors in human fracture healing

“…However, the findings of in vivo animal studies of the effect of LMWHs on fracture healing remain contentious and no conclusive findings can be drawn from these studies [6,8,9]. Nevertheless, in vitro studies on bone cells [10][11][12] and in vitro studies on bone metabolism [6,[13][14][15][16][17] demonstrate that LMWHs decrease bone formation and, therefore, could potentially delay fracture healing.…”