Effect of Low-Level Treatment with an 80-Hz Pulsed Infrared Diode Laser on Mast-Cell Numbers and Degranulation in a Rat Model of Third-Degree Burn

Khoshvaghti, Amir; Zibamanzarmofrad, Maral; Bayat, Mohammad

doi:10.1089/pho.2010.2783

Cited by 29 publications

(27 citation statements)

References 29 publications

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“…Our results and interpretation are supported by previous evidence in which Assis et al demonstrated that NIR 808 nm laser at a dose of 180 J/cm 2 , 3.8 mW/cm 2 decreases NF-kB expression, which led to reduce TNF-α level in cryolesion of tibialis anterior muscle in rats [35]. In another study, LLLT with 80 Hz pulsed NIR 890 nm laser significantly decreased the total number of the mast cells and degranulation during the proliferation and remodeling phase in third-degree burns in rats [10]. …”

Section: Discussionmentioning

confidence: 99%

Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats

et al. 2016

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Chronic non-healing cutaneous wounds are often vulnerable in one or more repair phases that prevent normal healing and pose challenges to the use of conventional wound care modalities. In immunosuppressed subject, the sequential stages of healing get hampered, which may be the consequences of dysregulated or stagnant wound inflammation. Photobiomodulation (PBM) or low-level laser (light) therapy (LLLT) emerges as a promising drug-free, non-invasive biophysical approach for promoting wound healing, reduction of inflammation, pain and restoration of functions. The present study was therefore undertaken to evaluate the photobiomodulatory effects of 810 nm diode laser (40 mW/cm2; 22.6 J/cm2) with pulsed (10 and 100 Hz, 50% duty cycle) and continuous wave on full-thickness excision-type dermal wound healing in hydrocortisone-induced immunosuppressed rats. Results clearly delineated that 810 nm PBM at 10 Hz was more effective over continuous and 100 Hz frequency in accelerating wound healing by attenuating the pro-inflammatory markers (NF-kB, TNF-α), augmenting wound contraction (α-SM actin), enhancing cellular proliferation, ECM deposition, neovascularization (HIF-1α, VEGF), re-epithelialization along with up-regulated protein expression of FGFR-1, Fibronectin, HSP-90 and TGF-β2 as compared to the non-irradiated controls. Additionally, 810 nm laser irradiation significantly increased CCO activity and cellular ATP contents. Overall, the findings from this study might broaden the current biological mechanism that could be responsible for photobiomodulatory effect mediated through pulsed NIR 810 nm laser (10 Hz) for promoting dermal wound healing in immunosuppressed subjects.

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Section: Discussionmentioning

confidence: 99%

Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats

et al. 2016

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“…In burn injury, the entire epidermis and most of the dermis are affected which produces a hypertrophic scar and inflexibility which leads to serious cosmetic problems with consequences in haemostasis, inflammation process, reepithelialisation and extracellular matrix remodelling [1]. As consequence, there is increasing interest in therapies that accelerate the healing of burn injuries, increase the quality of the scar and reduce the treatment cost [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, low-level laser therapy (LLLT) has been used for the treatment of burn injuries [1,3,[15][16][17][18][19]. Knowledge of the depth penetration of laser radiation in tissue is an essential prerequisite for identifying its method of action [20].…”

Section: Introductionmentioning

confidence: 99%

InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries

Catarino¹,

Godoy²,

Scharlack³

et al. 2015

Lasers Med Sci

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Therapies that accelerate the healing of burn injuries, improving the quality of life of the patient and reducing the cost of treatment are important. This study evaluated the effects of InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis leaves on burn wound healing in rats. Seventy-two rats were divided randomly into four groups: control untreated (C), treated with InGaP 670-nm laser with power density of 0.41 W/cm(2) and energy density of 4.93 J/cm(2) (L), treated with S. chilensis extract (S) and treated with S. chilensis extract and laser (LS). Second-degree burns were produced on the back of the animals with metal plate. Wound samples were collected on days 7, 14 and 21 of treatment for structural analysis, morphometry and Western blotting to quantify the expression of transforming growth factor beta 1 (TGF-β1) and vascular endothelial growth factor (VEGF). The results showed that InGaP laser irradiation at 670 nm alone and combined with extract of S. chilensis promoted significant tissue repair responses in this experimental model, increasing the number of fibroblasts, collagen fibres and newly formed blood vessels throughout the experimental period and decreasing the number of granulocytes in burn wounds of second degree in all treated groups. Exclusive treatment of burn wounds with the hydroalcoholic extract of S. chilensis provided similar quantitative results to those seen in the untreated group throughout the experimental period. Therefore, it was observed in the L and LS groups different responses in the expression of TGF-β1 and VEGF. The application of 670-nm laser alone or combined with the extract of S. chilensis promoted favourable responses in tissue repair of second-degree burns in this experimental model.

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“…On day 13, there were significantly lower numbers of types I and II mast cells at the laser-treated burn sites compared with the other burn sites. Khoshvaghti et al [38] concluded that LLLT significantly declined total numbers of mast cells through the proliferation and remodeling phases of healing in a rat model of third-degree burn. Ezzati et al investigated the influence of PW LLLT on healing of a deep second-degree burn model in rats.…”

Section: Skin Burn Healingmentioning

confidence: 99%

The Need for Increased Attention to Low‐Level Laser Therapy as Treatment for Wounds and Ulcers

Bayat¹

2016

Wound Healing - New Insights Into Ancient Challenges

Self Cite

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Light amplification by stimulated emission of radiation (lasers) is a device that typically generates electromagnetic radiation of uniform wavelength, phase, and polarization. The term low-level laser therapy (LLLT) is broadly defined as the therapeutic benefit of lasers. This review aims to discuss the positive effects of LLLT on skin wounds, diabetic foot ulcers, and burn healing. Different LLLT protocols have been widely used as treatment for these conditions to accelerate tissue regenerative processes. We have classified eligible papers in the fields of skin wounds, ulcers, and burns into in vivo and in vitro experimental studies and clinical trials that evaluated the use of LLLT as treatments that promote healing. An electronic search of scientific peer reviewed papers was conducted in the PubMed database. Our search has shown that the use of LLLT in biology and medicine is growing rapidly, and advancements in LLLT research dramatically improved the clinicians' ability to safely and effectively treat wounds and ulcers. There is increased clinical use of laser for wound and ulcer treatment. Several recent studies have confirmed the potential beneficial effects of LLLT for wound healing.

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Effect of Low-Level Treatment with an 80-Hz Pulsed Infrared Diode Laser on Mast-Cell Numbers and Degranulation in a Rat Model of Third-Degree Burn

Abstract: We conclude that LLLT can significantly decrease total numbers of mast cells during the proliferation and remodeling phases of healing in a rat model of third-degree burn.

Cited by 29 publications

References 29 publications

Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats

Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats

InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries

The Need for Increased Attention to Low‐Level Laser Therapy as Treatment for Wounds and Ulcers

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