Effect of Low-Level Laser Therapy on Human Adipose-Derived Stem Cells: In Vitro and In Vivo Studies

Min, Ki‐Bok; Byun, Jin Hwan; Heo, Chan Yeong; Kim, Eun Hee; Choi, Hye Yeon; Pak, Chang Sik

doi:10.1007/s00266-015-0524-6

Cited by 33 publications

(21 citation statements)

References 30 publications

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“…Cell proliferation can be positively influenced by LLLT as already shown in various studies (Emelyanov & Kiryanova, ; Li, Chen, & Huang, ; Lipovsky, Oron, Gedanken, & Lubart, ; Min et al, ). This is in consistence with our findings of increased sizes of colonies in the CFU‐F assay.…”

Section: Discussionmentioning

confidence: 52%

Photobiomodulation of freshly isolated human adipose tissue-derived stromal vascular fraction cells by pulsed light-emitting diodes for direct clinical application

Priglinger

Maier

Chaudary

et al. 2018

J Tissue Eng Regen Med

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A highly interesting source for adult stem cells is adipose tissue, from which the stromal vascular fraction (SVF)-a heterogeneous cell population including the adipose-derived stromal/stem cells-can be obtained. To enhance the regenerative potential of freshly isolated SVF cells, low-level light therapy (LLLT) was used. The effects of pulsed blue (475 nm), green (516 nm), and red (635 nm) light from light-emitting diodes applied on freshly isolated SVF were analysed regarding cell phenotype, cell number, viability, adenosine triphosphate content, cytotoxicity, and proliferation but also osteogenic, adipogenic, and proangiogenic differentiation potential. The colony-forming unit fibroblast assay revealed a significantly increased colony size after LLLT with red light compared with untreated cells, whereas the frequency of colony-forming cells was not affected. LLLT with green and red light resulted in a stronger capacity to form vascular tubes by SVF when cultured within 3D fibrin matrices compared with untreated cells, which was corroborated by increased number and length of the single tubes and a significantly higher concentration of vascular endothelial growth factor. Our study showed beneficial effects after LLLT on the vascularization potential and proliferation capacity of SVF cells. Therefore, LLLT using pulsed light-emitting diode light might represent a new approach for activation of freshly isolated SVF cells for direct clinical application.

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Section: Discussionmentioning

confidence: 52%

Photobiomodulation of freshly isolated human adipose tissue-derived stromal vascular fraction cells by pulsed light-emitting diodes for direct clinical application

Priglinger

Maier

Chaudary

et al. 2018

J Tissue Eng Regen Med

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“…Their in vivo results also revealed elevated numbers of stem cells compared to the control group [116]. Epidermal stem cells can also be influenced by light, as demonstrated by Liao et al The authors reveal that 632.8 nm light has photobiological effects on cultured human epidermal stem cells, such as an increase in proliferation and migration in vitro [110].…”

Section: Molecular Mechanisms Of Pbmmentioning

confidence: 99%

Proposed Mechanisms of Photobiomodulation or Low-Level Light Therapy

Freitas

Hamblin

2016

IEEE J. Select. Topics Quantum Electron.

1,018

796

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Photobiomodulation (PBM) also known as low-level laser (or light) therapy (LLLT), has been known for almost 50 years but still has not gained widespread acceptance, largely due to uncertainty about the molecular, cellular, and tissular mechanisms of action. However, in recent years, much knowledge has been gained in this area, which will be summarized in this review. One of the most important chromophores is cytochrome c oxidase (unit IV in the mitochondrial respiratory chain), which contains both heme and copper centers and absorbs light into the near-infra-red region. The leading hypothesis is that the photons dissociate inhibitory nitric oxide from the enzyme, leading to an increase in electron transport, mitochondrial membrane potential and ATP production. Another hypothesis concerns light-sensitive ion channels that can be activated allowing calcium to enter the cell. After the initial photon absorption events, numerous signaling pathways are activated via reactive oxygen species, cyclic AMP, NO and Ca2+, leading to activation of transcription factors. These transcription factors can lead to increased expression of genes related to protein synthesis, cell migration and proliferation, anti-inflammatory signaling, anti-apoptotic proteins, antioxidant enzymes. Stem cells and progenitor cells appear to be particularly susceptible to LLLT.

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“…( 3 - 5 ) The only study assessing the effect of LLLI with wavelengths higher than 800nm on ADSC showed a 830nm laser (0.05J/cm 2 ) promoted an increase in cell proliferation of ADSCs compared to the Control Group. ( 7 )…”

Section: Discussionmentioning

confidence: 99%

“…In this regard, several studies have evaluated the effect of low-level laser irradiation (LLLI) on the proliferation of mesenchymal stem cells (MSC) obtained from different sources, ( 2 ) and satisfactory results were reported on ADSC. ( 3 - 7 ) Thus, laser therapy has emerged as an alternative to stimulate in vitro cell proliferation.…”

Section: Introductionmentioning

confidence: 99%

Low-level laser irradiation promotes proliferation of cryopreserved adipose-derived stem cells

Ginani

Soares

Rocha

et al. 2017

Einstein (São Paulo)

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Objective To evaluate the effect of low-level laser irradiation on proliferation and viability of murine adipose-derived stem cells previously submitted to cryopreservation.Methods Adipose-derived stem cells were isolated from inguinal fat pads of three mice, submitted to cryopreservation in fetal bovine serum with 10% dimethylsulfoxide for 30 days and then thawed and maintained in normal culture conditions. Culture cells were either irradiated or not (control) with an InGaAIP diode laser at zero and 48 hours, using two different energy densities (0.5 and 1.0J/cm2). Cell proliferation was evaluated by trypan blue exclusion method and MTT assay at intervals of zero, 24, 48, and 72 hours after the first laser application. Cell viability and apoptosis of previously cryopreserved cells submitted to laser therapy were evaluated by flow cytometry.Results The Irradiated Groups (0.5 and 1.0J/cm2) showed an increased cell proliferation (p<0.05) when compared to the Control Group, however no significant difference between the two energy densities was observed. Flow cytometry revealed a percentage of viable cells higher than 99% in all groups.Conclusion Low-level laser irradiation has stimulatory effects on the proliferation of adipose-derived stem cells previously submitted to cryopreservation.

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Effect of Low-Level Laser Therapy on Human Adipose-Derived Stem Cells: In Vitro and In Vivo Studies

Cited by 33 publications

References 30 publications

Photobiomodulation of freshly isolated human adipose tissue-derived stromal vascular fraction cells by pulsed light-emitting diodes for direct clinical application

Photobiomodulation of freshly isolated human adipose tissue-derived stromal vascular fraction cells by pulsed light-emitting diodes for direct clinical application

Proposed Mechanisms of Photobiomodulation or Low-Level Light Therapy

Low-level laser irradiation promotes proliferation of cryopreserved adipose-derived stem cells

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