Effect of low-level laser therapy on wound healing after depigmentation procedure: A clinical study

Abstract

Aim:The aim of the present study is to evaluate and compare the effects of low-level laser therapy (LLLT) on wound healing after depigmentation procedure.Materials and Methods:In this study, 12 patients with bilateral melanin hyperpigmentation were treated with surgical stripping using a blade. After completion of the surgical process and bleeding stasis, any of the symmetrical surgical sites was randomly assigned for LLLT (test site) using a defocused diode laser at 1 mm distance for 5 min. After every laser exposure, the surgical site was coated with plaque disclosing solution (erythrosine) on the 3rd, 7th, and 15th day. A photograph of the surgical site was taken using a Digital SLR Camera, which was placed at 30 cm distance at 55 mm zoom, 1/100 shutter speed, f 14 aperture size, and ISO 4000 with a ring flash. The area of the stained parts of the photographs was evaluated using image analysis software.Results:At day 3, test site showed 1.26 ± 0.23 mm2 and control site showed 1.45 ± 0.21 mm2 stain uptake by the tissue which was statistically significant. At day 7 and day 15, the test sites exhibited 1.24 ± 0.30 mm2 and 1.12 ± 0.25 mm2 stain uptake, whereas the control site showed 1.37 ± 25 mm2 and 1.29 ± 0.28 mm2 staining, respectively, which were not statistically significant.Conclusion:Within the limitations of this study, the findings revealed that LLLT promotes wound healing after depigmentation procedure until the 3rd day. On the 7th and 15th day, the difference in healing was not statistically significant.

Diode Laser Assisted Excision and Low Level Laser Therapy in the Management of Mucus Extravasation Cysts: A Case Series

“…22 VAS recorded in present cases showed that perceived pain was highest at the first post-surgical day and decreased to pre-surgical levels after 1 week. This is in accordance with healing patterns of mucosal wounds as demonstrated by Kalakonda et al 19 and Chawla et al 23 who performed LLLT after vestibuloplasty procedure and after gingival depigmentation respectively. Since present case series did not have any controls, any conclusion about role of LLLT in perceived pain cannot be drawn.…”

“…21 Healing of surgical wound has been found to be promoted by LLLT in many studies. [21][22][23] Various mechanisms have been proposed to explain this effect of LLLT. 22 At the cellular level, low power laser causes photochemical, photophysical and/or photobiological effects in the cells, leading to stimulation of certain cellular functions, particularly increased ATP production in the mitochondria.…”

Terapia a laser de baixa potência na cicatrização de feridas

“…27 It was observed that in the postoperative wound, the laser promoted healing until the 3rd day, but in the 7th and 15th days, the difference in healing was not statistically significant. 32 A similar difference was found in another study that observed that the laser is linked to the increase in fibroblastic activities, but without statistical difference in the healing time of the oral mucosa after a gingivoplasty procedure. It was referred, through these findings, the need for new immunohistological studies correlating the clinical alterations to findings at the cellular level.…”

“…It was identified that 11 articles mentioned about the action of the laser in the cellular growth. 5,[18][19][20][23][24][25][26][27][31][32] This action is related to the mechanisms of the proliferative phase of cicatrization, which corresponds to the formation of the granulation tissue and the new vessels of the cell matrix. 33 It is inferred that there was also positive evidence related to re-epithelialization of chronic wounds and the acceleration of healing, as well as decrease of pain during treatment.…”

Atomic Force Microscopy on Biological Materials Related to Pathological Conditions

“…Low-level laser therapy (LLLT), or photobiomodulation [125], applies low-level (also referred and as low-power) lasers for the treatment of several pathological conditions or abnormalities, including fibromyalgia, osteoarthritis, tuberculosis, temporomandibular joint disorders, and wound healing that presents increased interest. In the case of wound healing, it has been suggested that the underling mechanism, which remains partially unknown, includes the increase of mitochondrial function, ATP, RNA, and protein synthesis [126]. Subsequently, these lead to increased oxygen consumption and enhanced synthesis of the reduced form of nicotinamide adenine dinucleotide (NADH) and ATP.…”