Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells

Abstract

The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm(2) using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm(2) on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.

The influence of continuous low-intensity laser radiation at the red (635 nm) and green (525 nm) wavelengths on the human mesenchymal stem cells in vitro: a review of the literature and original investigations

“…В некоторых работах [24][25][26] положительные результаты воздействия НИЛИ с длиной волны 635 нм достигаются при значительно большей ЭП 5 -Дж/см 2 , но касаются они стимулирования высво-бождения клетками эпидермального фактора роста (EGF). Возможно, различные физиологические проявления клеточной активности имеют место при вариациях энергетических и спектральных характе-ристик лазерного света.…”

The role of adipose derived stem cells, smooth muscle cells and low intensity laser irradiation (LILI) in tissue engineering and regenerative medicine

“…They have also been differentiated into smooth muscle cells that are functional [27]. These cells have also shown increased viability and proliferation when exposed to LILI alone [5] and, as well as, in combination with epidermal growth factor (EGF) [28]. Recent studies have demonstrated the functional neural differentiation of human ADSCs [29].…”

Inducing stem cell differentiation using low intensity laser irradiation: a possible novel therapeutic intervention

“…In addition, LILI has been shown to illicit different responses in different cell types depending on the intensity of irradiation. Molecular and cellular processes are inhibited or stimulated at different intensities of LILI as indicated by changes in metabolism, increased adenosine triphosphate (ATP) production and increased cellular migration [14]. Furthermore, LILI has also been shown to promote the proliferation of rat mesenchymal bone marrow, cardiac and ADSCs in vitro [14,15].…”

“…Molecular and cellular processes are inhibited or stimulated at different intensities of LILI as indicated by changes in metabolism, increased adenosine triphosphate (ATP) production and increased cellular migration [14]. Furthermore, LILI has also been shown to promote the proliferation of rat mesenchymal bone marrow, cardiac and ADSCs in vitro [14,15]. It was recently demonstrated that not only do ADSCs respond well to external stimuli, including LILI, as a mechanism of inducing increased viability and proliferation, but differentiation into other cell lineages can be achieved with minimal invasive techniques such as careful selection and addition of growth factor combinations.…”