Effect of low level capacitive‐coupled pulsed electric field stimulation on mineral profile of weight‐bearing bones in ovariectomized rats

Abstract

Mineral content, mineral composition, and crystalline pattern of bone in osteoporosis are different from those of normal individuals. Present management of bone mineral loss is rather unsatisfactory primarily because of socioeconomic factors and untoward effects of the treatment drugs. We report the efficacy of capacitive-coupled pulsed electric field (CCPEF) to prevent bone loss in an ovariectomized rat model of osteoporosis. One month postsurgery either leg was stimulated with CCPEF, whereas the other leg did not receive any stimulation (sham exposed). The treatment was given in 60 sessions each of 2 h/d (5 days a week). The control group of rats was sham operated. At the end of the observation period, femur and tibia bones were removed. Their bone mineral content (BMC), calcium, phosphorus, and carbon contents were analyzed and bone mineral density (BMD) was calculated. The BMC data were supported by X-ray diffraction (XRD) method. In sham-exposed bones, a statistically significant decrease in BMC, BMD, calcium, and phosphorus contents were obtained as compared to the control. Although in CCPEF bones, there was an attenuation of decrement in the noted parameters except phosphorus. XRD pattern supported these observations. The results suggest that chronic, 60 sessions of 2 h/d, 5 d/wk CCPEF (14 MHz with 16 Hz modulation 16 Hz and 10 V peak to peak) is effective in attenuating the ovariectomy-induced bone mineral loss in rats.

Electrical Stimulation in Tissue Regeneration

“…The principal endpoints include bone mineral content/density, and the content of calcium, phosphorus, and carbon, as well as mechanical properties. (Manjhi et al, 2009). …”

The therapeutic effect of a pulsed electromagnetic field on the reproductive patterns of male Wistar rats exposed to a 2.45-GHz microwave field

“…Following the experiments of DiCarlo et al26 on chick embryos exposed to radiation, the protection conferred by the magnetic fields appears to influence free radical scavenging. PEMF has a variety of biological effects, such as its effects on bone healing,27,28 pain relief,29 and the balance of the neuroendocrine system (including hormone production and melatonin levels) 30. PEMF has frequencies at the lower end of the electromagnetic spectrum (from 6 Hz to 500 Hz).…”

Physical Stimulations for Bone and Cartilage Regeneration

“…In 1953, Yasuda et al applied continuous electrical current to a rabbit femur for 3 weeks and demonstrated new bone formation around the cathode [44]. Since then, use of EFs for bone healing applications has been widely researched [45]. Capacitive coupling electric field (CCEF) and inductive coupling electromagnetic field (EMF) are also being used more frequently in recent years.…”