Effect of Low‐Intensity Pulsed Ultrasound on Bone Regeneration

Abstract

Objectives The purpose of this study was to evaluate the effects of low‐intensity pulsed ultrasound at 1.0 MHz on the healing process of fractures with bone loss in the rat fibula by alkaline phosphate level measurement and radiologic analyses. Methods Thirty 70‐day‐old male Wistar rats underwent a bone resection of 2.5 to 3.0 mm between the proximal and middle third of the right fibular diaphysis. The animals were randomly divided into 3 experimental groups: reference (uninjured), control (injured only), and treated (injured and treated with 5 applications of ultrasound, interspersed by 2 days of rest, beginning 24 hours after the osteotomy). Euthanasia was performed at experimental periods of 7 and 14 days. The right hind limb was removed for radiologic analysis. The blood was collected via cardiac puncture to determine the serum alkaline phosphatase activity. Results The bone fractures had not been completely consolidated in the treated and control group when analysis of the bone took place. At day 7, the serum alkaline phosphatase activity was higher in the treated group (mean ± SD, 72.17 ± 7.02 U/L) compared to the control (65.26 ± 8.41 U/L) and reference (67.21 ± 7.86 U/L) groups. At day 14, higher alkaline phosphatase activity was seen in the control group (68.96 ± 8.12 U/L) compared to the treated (66.09 ± 8.46 U/L) and reference (67.14 ± 7.96 U/L) groups. Conclusions The biochemical and radiologic results suggest that low‐intensity pulsed ultrasound can be used as an auxiliary method to consolidate fractures and probably reduces the bone healing time, offering clinical benefits.

Effects of low-level laser therapy on autogenous bone graft stabilized with a new heterologous fibrin sealant

“…Several studies have demonstrated that physical methods can augment bone regeneration in order to enhance this healing process [28][29][30]. Low-level laser therapy is effective mainly due to its positive effects on bone metabolism and consolidation of fractures via osteoblastic stimulation and increased bone mineral density [31][32][33][34].…”

Elastin-derived scaffolding associated or not with bone morphogenetic protein (BMP) or hydroxyapatite (HA) in the repair process of metaphyseal bone defects

“…Based on current results and the perspective of new analyzes of the performance of the bone substitute, with a view to reducing the time of effective functional recovery and reinforcing the translational potential of preclinical research, therapies such as pulsed ultrasound (LIPUS) and laser photobiomodulation therapy (PBMT) can be added, which can allow a significant impact in reducing health care costs [42][43][44][45].…”

Enhancement of Neurotrophic Factors in Astrocyte for Neuroprotective Effects in Brain Disorders Using Low-intensity Pulsed Ultrasound Stimulation

“…Low-intensity pulsed US (LIPUS) has been known to accelerate bone and tissue regeneration following injury [4,5]. In past studies, kidneys, monocytes, and osteoblasts treated with focused US (FUS) have shown significant increases in vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF), which are known to promote hippocampal neurogenesis [6,7].…”