Effect of Low Intensity Helium-Neon (He-Ne) Laser Irradiation on Diabetic Wound Healing Dynamics

Abstract

Laser photostimulation promotes the tissue repair process of diabetic wounds.

Comparison between Wound Healing in Induced Diabetic and Nondiabetic Rats after Low-Level Laser Therapy

“…[6][7][8][9][10][11][12][13] Some lasers, when used with the proper parameters, affect fibroblasts and collagen production in vitro, and promising results have been found utilizing the HeNe laser (632.8 nm) with multiple exposures. 9,[14][15][16][17][18][19] One effect of laser therapy (5 J/cm 2 ) is increased local blood flow in normal human skin of the sole of the foot. 6 A comparative study of LLLT on fibroblasts in vitro, using fluence of 2 J/cm 2 and various irradiances, concluded that the proliferation rate of human gingival fibroblasts was increased when under conditions of nutritional deficit and stress.…”

Uso combinado da laserterapia de baixa potência e da inibição da ciclooxigenase-2 na reepitelização de ferida incisional em pele de camundongos: um estudo pré-clínico

“…21 -23 According to Karu et al 17.24 and other authors [20][21][22][23] , exposure to LLLT stimulates an extra mitochondrial electrochemical activity and a simultaneous increase in the synthesis of adenosine triphosphate (ATP), in addition to an increase in the intracellular concentration of calcium (Ca +2 ), exerting effects on cellular signaling and increasing the activity of NOS in endothelial cells, which would offer cytoprotection to damage caused by lipid peroxidation induced by LLLT. [14][15][16] A B C D …”

“…1,2,[11][12][13][14] Recent investigations show the influence of LLLT in the dynamics of healing of the skin and other tissues; however, there are still some controversies about it because of the wide variability and divergence in irradiation parameters, in addition to discrepancies in the biological findings. [14][15][16] Previous studies in our laboratory as well as others have suggested that photo-stimulation by laser seems to occur during the inflammatory and proliferative phases of wound healing, exactly because of their influence on biomodulation of eicosanoids, especially PGs, and on the oxidative enzyme system of the skin. 1,2,5,[11][12][13][14][15][16][17][18][19] Consequently, studies that show the effects of combined treatments in wound healing are opportune, since both laser therapy and the use of COX inhibitors act during the same time period in the repair process, that is, both separate treatments affect the inflammatory and proliferative phases of skin healing in animals and humans.…”

“…[14][15][16] Previous studies in our laboratory as well as others have suggested that photo-stimulation by laser seems to occur during the inflammatory and proliferative phases of wound healing, exactly because of their influence on biomodulation of eicosanoids, especially PGs, and on the oxidative enzyme system of the skin. 1,2,5,[11][12][13][14][15][16][17][18][19] Consequently, studies that show the effects of combined treatments in wound healing are opportune, since both laser therapy and the use of COX inhibitors act during the same time period in the repair process, that is, both separate treatments affect the inflammatory and proliferative phases of skin healing in animals and humans. Considering this scenario, the purpose of this pre-clinical study was to evaluate the isolated and combined effects of low level laser therapy (LLLT) with helium-neon (He-Ne, 632nm) and selective inhibition of cyclooxygenase-2 (COX-2) in the reepithelialization of skin-incisional wounds in mice.…”

Effect of gallium-arsenide laser, gallium-aluminum-arsenide laser and healing ointment on cutaneous wound healing in Wistar rats

“…In vitro studies on fibroblast laser irradiation have reported a proliferative effect on protein synthesis depending on the characteristics and parameters of the type of laser used, such as wavelength, form of emission and energy density (12,13).…”