Effect of Low Intensity Helium–Neon (HeNe) Laser Irradiation on Experimental Paracoccidioidomycotic Wound Healing Dynamics

Abstract

The effect of HeNe laser on the extracellular matrix deposition, chemokine expression and angiogenesis in experimental paracoccidioidomycotic lesions was investigated. At days 7, 8 and 9 postinfection the wound of each animal was treated with a 632.8 nm HeNe laser at a dose of 3 J cm . At day 10 postinfection, the wounds were examined by using histologic and immunohistochemical methods. Results revealed that lasertreated lesions were lesser extensive than untreated ones, and composed mainly by macrophages and lymphocytes. High IL-1b expression was shown in the untreated group whereas in lasertreated animals the expression was scarce. On the other hand, the expression of CXCL-10 was found to be reduced in untreated animals and quite intensive and well distributed in the lasertreated ones. Also, untreated lesions presented vascular endothelial growth factor (VEGF) in a small area near the center of the lesion and high immunoreactivity for hypoxia-inducible factor-1 (HIF-1), whereas laser-treated lesions expressed VEGF surrounding blood vessels and little immunoreactivity for HIF-1. Laser-treated lesions presented much more reticular fibers and collagen deposition when compared with the untreated lesion. Our results show that laser was efficient in minimizing the local effects observed in paracoccidioidomycosis and can be an efficient tool in the treatment of this infection, accelerating the healing process.

Low level laser therapy (AlGaInP) applied at 5J/cm2 reduces the proliferation of Staphylococcus aureus MRSA in infected wounds and intact skin of rats*

“…This demonstrated that laser therapy increases the phagocytic capacity of macrophages, facilitating cleaning of the wound. 22 Although the literature questions the influence of the environment around the bacteria and the application of laser in such circumstances, our study did not observe statistically significant differences when comparing the use of lasers in intact skin and wounds, resulting in a significant reduction in both cases. 6,13,21,22 …”

Histomorphometric Evaluation of the Effects of Various Diode Lasers and Force Levels on Orthodontic Mini Screw Stability

“…23 It is described as ranging from 4 to 10 J/cm 2 for soft tissues. [35][36][37][38][39] In light of this information, the laser doses selected were 10 and 20 J/cm 2 in this investigation. Although some researchers reported that there is no relationship between the cortical bone thickness and the stability of the mini screw, [40][41][42] others reported an opposing argument.…”

Efeitos da aplicação do L.A.S.E.R. HeNe e do ultravioleta B no vitiligo

“…Após a determinação do tempo, foi escolhida uma mácula e aplicado o UVB perpendicular com 10 cm de afastamento da pele O L.A.S.E.R. que pode ser utilizado para tratamento dessa patologia é o HeNe, que é de baixa energia e emite radiação em espectro de luz visível (10). Estudos demonstram que, além de modulação dos queratinócitos, ibroblastos e melanoblastos, o tratamento induz a síntese do fator de melanócitos relacionados com o crescimento, estimulando a proliferação de melanócitos.…”

“…A etiologia e patogenia exata da doença são desconhecidas. Existem teorias como as hipóteses neural, imune e autodestrutiva (6)(7)(8)(9)(10). A caracterização do vitiligo sugere que fatores predisponentes (genéticos) e precipitantes (ambientais) tornam uma pessoa mais suscetível à doença.…”