Effect of low glucose concentration on synaptic transmission in the rat hippocampal slice

Fan, Pengcheng; O’Regan, Patrick A.; Szerb, John C.

doi:10.1016/0361-9230(88)90041-x

Cited by 24 publications

(14 citation statements)

References 21 publications

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“…In this study, increased blood glucose level observed in diabetic model and decreased blood glucose level or hypoglycemia in hypoglycemic group confirmed the altered glucose homeostasis. Low glucose concentration in hypoglycemic rats interrupts synaptic transmission by reducing, but not abolishing, the excitability of neuronal cells (Fan et al 1988). Neurons of the CNS are exquisitely sensitive to the prevailing blood glucose concentration, with even moderate hypoglycemia resulting in significant impairment of neuronal function (Deary 1993).…”

Section: Discussionmentioning

confidence: 98%

Decreased Cholinergic Receptor Expression in the Striatum: Motor Function Deficit in Hypoglycemic and Diabetic Rats

et al. 2011

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Hypoglycemic brain injury is a common and serious complication of insulin therapy associated with diabetes. This study evaluated the effect of insulin-induced hypoglycemia and STZ-induced diabetes on striatal cholinergic receptors and enzyme expression and on motor function. Cholinergic enzymes: AChE and ChAT gene expression, radioreceptor binding assay and immunohistochemistry of muscarinic M1, M3 receptors and α7nAChR were carried out. Motor performance on grid walk test was analysed. AChE and ChAT expression significantly downregulated in hypoglycemic and diabetic rats. Total muscarinic and Muscarinic M3 receptor binding decreased in hypoglycemic rats compared to diabetic rats whereas muscarinic M1 receptor binding increased in hypoglycemic rats compared to diabetic rats. Real-time PCR analysis and confocal imaging of muscarinic M1, M3 receptors confirmed the changes in muscarinic receptor binding in hypoglycemic and diabetic rats. In hypoglycemic rats, α7nAChR expression significantly up regulated compared to diabetic rats. Grid walk test demonstrated the impairment in motor function and coordination in hypoglycemic and hyperglycemic rats. Neurochemical changes along with the behavioral data implicate a role for impaired striatal cholinergic receptor function inducing motor function deficit induced by hypo and hyperglycemia. Hypoglycemia exacerbated the neurobehavioral deficit in diabetes which has clinical significance in the treatment of diabetes.

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Section: Discussionmentioning

confidence: 98%

Decreased Cholinergic Receptor Expression in the Striatum: Motor Function Deficit in Hypoglycemic and Diabetic Rats

et al. 2011

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“…In rats, insulin-induced hypoglycemia reduces ATP and elevates adenosine levels (Chapman et al, 1981). In the rat hippocampal slice, hypoglycemia reduces ATP and depresses the evoked synaptic potential (Fan et al, 1988;Okada, 1988).…”

Section: Glucose Deprivation Results In a Lactate Preventable Increasmentioning

confidence: 99%

Glucose Deprivation Results in a Lactate Preventable Increase in Adenosine and Depression of Synaptic Transmission in Rat Hippocampal Slices

Fowler

1993

Journal of Neurochemistry

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: The effect of glucose deprivation on adenosine levels and on synaptic transmission was investigated in rat hippocampal slices. Incubation of hippocampal slices either in glucose‐free medium or in the presence of the glucose transport inhibitor cytochalasin B (50 μM) increased bath adenosine levels and depressed the extracellularly recorded synaptic potential or population spike. The addition of lactate (10 mM), a precursor for mitochondrial ATP generation, prevented the elevation in adenosine and the depression of the population spike. These results indicate that the neuroinhibitory modulator adenosine is elevated during glucose deprivation and contributes to the hypoglycemic depression of synaptic transmission. The increase in adenosine during glucose deprivation can be prevented by providing substrate for mitochondrial ATP generation. The present results indicate an interaction between lactate and adenosine such that an increase in lactate may contribute to a decline in adenosine production.

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“…Perfusion with 1mM glucose (down from 5mM) reversibly suppresses extracellular excitatory postsynaptic potentials in the cornu ammonis 1 region of hippocampal slices induced by electrical stimulation of Schaffer collaterals in slices from both euglycemic 19 and diabetic 20 rats. Similar data were collected using intracellular recordings in the dorsolateral septal nucleus in euglycemic rats.…”

Section: Annmentioning

confidence: 99%

Correlation between extracellular glucose and seizure susceptibility in adult rats

Schwechter

Velı́šková

Velı́šek

2002

Annals of Neurology

107

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In adult diabetic patients, periods of hyperglycemia may be associated with exacerbation of focal seizures. Our objective was to determine in the adult rats the correlation between seizure susceptibility and extracellular glucose concentration in two models of seizures. Male rats were injected with two doses of streptozocin (40 mg/kg IP) on 2 consecutive days to induce diabetic hyperglycemia. Controls either received vehicle or were not injected. After 2 weeks, blood glucose concentration was measured, and the rats were subjected to flurothyl seizure test. Another group of rats received glucose solution (20%, 5 ml IP) 30 minutes before testing to induce nondiabetic hyperglycemia. Thresholds for flurothyl-induced clonic and tonic-clonic seizures were determined. Finally, in vitro epileptiform activity was induced in the entorhinal cortex-hippocampal slices from naive rats by perfusing with magnesium-free medium with various glucose concentrations. In additional slices, paired-pulse paradigm was determined in the perforant path. Susceptibility to clonic and tonic-clonic flurothyl-induced seizures positively correlated with blood glucose concentrations as the increased glucose concentration was associated with proconvulsant effects. Similarly, in the in vitro experiments, epileptiform activity was promoted by increased and suppressed by decreased glucose concentrations. Data indicate that, in the adult rats, high glucose concentrations are associated with proconvulsant effects.

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Effect of low glucose concentration on synaptic transmission in the rat hippocampal slice

Cited by 24 publications

References 21 publications

Decreased Cholinergic Receptor Expression in the Striatum: Motor Function Deficit in Hypoglycemic and Diabetic Rats

Decreased Cholinergic Receptor Expression in the Striatum: Motor Function Deficit in Hypoglycemic and Diabetic Rats

Glucose Deprivation Results in a Lactate Preventable Increase in Adenosine and Depression of Synaptic Transmission in Rat Hippocampal Slices

Correlation between extracellular glucose and seizure susceptibility in adult rats

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