Effect of Low‐dose Irradiation on Pregnant Mouse Haemopoiesis

Weinberg, Sharon L.; McCarthy, Emmeline G.; MacVittie, Thomas J.; Baum, Siegmund J.

doi:10.1111/j.1365-2141.1981.00127.x

Cited by 17 publications

(2 citation statements)

References 8 publications

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“…PBMCs were purified from venous blood (Ͻ3.0 ml; n ϭ 37) collected in EDTA-containing vials using Ficoll/Hypaque as described previously (45). Cells were plated at 1 ϫ 10 6 cells per ml in Dulbecco's modified Eagle's medium (DMEM) containing 10% pooled human serum (heat inactivated at 56°C for 30 min) and cultured for 48 h. SCGF genotyping.…”

Section: Methodsmentioning

confidence: 99%

A Novel Functional Variant in the Stem Cell Growth Factor Promoter Protects against Severe Malarial Anemia

et al. 2010

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Plasmodium falciparum malaria is a leading global cause of infectious disease burden. In areas in which P. falciparum transmission is holoendemic, such as western Kenya, severe malarial anemia (SMA) results in high rates of pediatric morbidity and mortality. Although the pathophysiological basis of SMA is multifactorial, we recently discovered that suppression of unexplored hematopoietic growth factors that promote erythroid and myeloid colony development, such as stem cell growth factor (SCGF) (C-type lectin domain family member 11A [CLEC11A]), was associated with enhanced development of SMA and reduced erythropoietic responses. To extend these investigations, the relationships between a novel SCGF promoter variant (؊539C/T, rs7246355), SMA (hemoglobin [Hb] < 6.0 g/dl), and reduced erythropoietic responses (reticulocyte production index [RPI], <2.0) were investigated with Kenyan children (n ‫؍‬ 486) with falciparum malaria from western Kenya. Circulating SCGF was positively correlated with hemoglobin levels (r ‫؍‬ 0.251; P ‫؍‬ 0.022) and the reticulocyte production index (RPI) (r ‫؍‬ 0.268; P ‫؍‬ 0.025). Children with SMA also had lower SCGF levels than those in the non-SMA group (P ‫؍‬ 0.005). Multivariate logistic regression analyses controlling for covariates demonstrated that individuals with the homologous T allele were protected against SMA (odds ratio, 0.57; 95% confidence interval [95% CI] 0.34 to 0.94; P ‫؍‬ 0.027) relative to CC (wild-type) carriers. Carriers of the TT genotype also had higher SCGF levels in circulation (P ‫؍‬ 0.018) and in peripheral blood mononuclear cell culture supernatants (P ‫؍‬ 0.041), as well as an elevated RPI (P ‫؍‬ 0.005) relative to individuals with the CC genotype. The results presented here demonstrate that homozygous T at ؊539 in the SCGF promoter is associated with elevated SCGF production, enhanced erythropoiesis, and protection against the development of SMA in children with falciparum malaria.

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Section: Methodsmentioning

confidence: 99%

A Novel Functional Variant in the Stem Cell Growth Factor Promoter Protects against Severe Malarial Anemia

et al. 2010

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“…PBMCs were purified from venous blood (Ͻ3.0 ml from Kenyan children [n ϭ 55] and 40 ml from malaria-naïve U.S. adults [n ϭ 11]) by use of Ficoll-Hypaque as described previously (48). Cells were plated at 1 ϫ 10 6 cells per ml in Dulbecco's modified Eagle's medium containing 10% pooled human serum (heat inactivated at 56°C for 30 min).…”

Section: Methodsmentioning

confidence: 99%

Suppression of a Novel Hematopoietic Mediator in Children with Severe Malarial Anemia

Keller

Ouma

et al. 2009

Infect Immun

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In areas of holoendemic Plasmodium falciparum transmission, severe malarial anemia (SMA) is a leading cause of pediatric morbidity and mortality. Although many soluble mediators regulate erythropoiesis, it is unclear how these factors contribute to development of SMA. Investigation of novel genes dysregulated in response to malarial pigment (hemozoin [PfHz]) revealed that stem cell growth factor (SCGF; also called C-type lectin domain family member 11A [CLEC11A]), a hematopoietic growth factor important for development of erythroid and myeloid progenitors, was one of the most differentially expressed genes. Additional experiments with cultured peripheral blood mononuclear cells (PBMCs) demonstrated that PfHz decreased SCGF/CLEC11A transcriptional expression in a time-dependent manner. Circulating SCGF levels were then determined for Kenyan children (n ‫؍‬ 90; aged 3 to 36 months) presenting at a rural hospital with various severities of malarial anemia. SCGF levels in circulation (P ‫؍‬ 0.001) and in cultured PBMCs (P ‫؍‬ 0.004) were suppressed in children with SMA. Circulating SCGF also correlated positively with hemoglobin levels (r ‫؍‬ 0.241; P ‫؍‬ 0.022) and the reticulocyte production index (RPI) (r ‫؍‬ 0.280; P ‫؍‬ 0.029). In addition, SCGF was decreased in children with reduced erythropoiesis (RPI of <2) (P < 0.001) and in children with elevated levels of naturally acquired monocytic PfHz (P ‫؍‬ 0.019). Thus, phagocytosis of PfHz promotes a decrease in SCGF gene products, which may contribute to reduced erythropoiesis in children with SMA.

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Removal of peripheral blood monocytes by phenylalanine methyl ester has no effect on the colony growth of hematopoietic progenitor cells

Law

Schwartz

Alsop

et al. 1989

The International Journal of Cell Cloning

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Purification of hematopoietic cells often includes an adherence step for the removal of monocytes. Recent studies have shown that some hematopoietic cells are adherent and that plastic adhesion might activate monocyte/macrophage for cytokine production. We investigated the possibility of using L-phenylalanine methyl ester (PME) as an alternative approach to monocyte removal, particularly for large-scale cell separations. Cell yield, extent of monocyte removal and cloning efficiency of colony-forming progenitor cells were compared to that produced by plastic adhesion. Starting with mononuclear cells (MNC) from adult peripheral blood, cell recovery after PME treatment was higher than that achieved with adherence and fewer monocytes were present. Cloning efficiency of the granulocyte-monocyte colony-forming progenitor cells (CFU-gm) was not affected by the PME treatment. PME treatment and plastic adhesion of low-density cord blood cells produced comparable cell recovery, monocyte depletion and cloning efficiencies for CFU-gm and burst forming units for erythroid progenitor cells (BFU-e). The PME procedure was optimized for the purification of hematopoietic cells from large quantities (less than 1 x 10(9)) of low density. T cell-depleted peripheral blood MNC containing 60% to 85% monocytes. Compared to two cycles of plastic adhesion, PME treatment permitted higher cell recovery (5.9% vs. 1.3%), lower monocyte contamination (4.5% vs. 10.7%) and substantial cost, time and labor savings without compromising CFU-gm growth.

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Effect of Low‐dose Irradiation on Pregnant Mouse Haemopoiesis

Cited by 17 publications

References 8 publications

A Novel Functional Variant in the Stem Cell Growth Factor Promoter Protects against Severe Malarial Anemia

A Novel Functional Variant in the Stem Cell Growth Factor Promoter Protects against Severe Malarial Anemia

Suppression of a Novel Hematopoietic Mediator in Children with Severe Malarial Anemia

Removal of peripheral blood monocytes by phenylalanine methyl ester has no effect on the colony growth of hematopoietic progenitor cells

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