Effect of low-dose ionizing radiation on spatiotemporal parameters of functional responses induced by electrical signals in tobacco plants

Grinberg, Marina; Nemtsova, Yuliya; Ageyeva, Maria; Brilkina, Anna A.; Vodeneev, Vladimir

doi:10.1007/s11120-023-01027-9

Cited by 3 publications

(1 citation statement)

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“…The presence of fluorescent proteins in the cytosol also enables the visualization of cell morphology and different tissue types without the need for histological staining. Pt-GFP, a widely used genetically encoded ratiometric pH sensor [23,26,27,29,[35][36][37], boasts a broad pH sensitivity range (pH 4.5-8.5), exceptional acid stability, and insensitivity to salinity [26]. In this study, we employed transgenic tobacco plants expressing a pH-sensitive sensor Pt-GFP to investigate how salinity affects pHcyt in root cells.…”

Section: Introductionmentioning

confidence: 99%

The Morphological Parameters and Cytosolic pH of Cells of Root Zones in Tobacco Plants (Nicotiana tabacum L.): Nonlinear Effects of NaCl Concentrations

Ageyeva,

Zdobnova,

Nazarova

et al. 2023

Plants

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Salinity impacts important processes in plants, reducing their yield. The effect of salinity on the cytosolic pH (pHcyt) has been little studied. In this research, we employed transgenic tobacco plants expressing the pH sensor Pt-GFP to investigate the alterations in pHcyt in cells across various root zones. Furthermore, we examined a wide spectrum of NaCl concentrations (ranging from 0 to 150 mM) and assessed morphological parameters and plant development. Our findings revealed a pattern of cytosolic acidification in cells across all root zones at lower NaCl concentrations (50, 100 mM). Interestingly, at 150 mM NaCl, pHcyt levels either increased or returned to normal, indicating a nonlinear effect of salinity on pHcyt. Most studied parameters related to development and morphology exhibited an inhibitory influence in response to NaCl. Notably, a nonlinear relationship was observed in the cell length within the elongation and differentiation zones. While cell elongation occurred at 50 and 100 mM NaCl, it was not evident at 150 mM NaCl. This suggests a complex interplay between stimulating and inhibitory effects of salinity, contributing to the nonlinear relationship observed between pHcyt, cell length, and NaCl concentration.

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