Effect of long-term treatment with classical neuroleptics on NPQ/spexin, kisspeptin and POMC mRNA expression in the male rat amygdala

Pałasz, Artur; Pałka, Marcelina; Filipczyk, Łukasz; Menezes, Itiana Castro; Rojczyk, Ewa; Worthington, John J.; Piwowarczyk-Nowak, Aneta; Krzystanek, Marek; Wiaderkiewicz, Ryszard

doi:10.1007/s00702-018-1868-2

Cited by 9 publications

(6 citation statements)

References 59 publications

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“…It is worth noting here that the physiological effect of olanzapine observed in the brainstem turned out to be identical to that of escitalopram (an antidepressant) in the hypothalamus. Our results, however, do not correspond with increased NPQ/spexin mRNA expression in the amygdala of rats exposed to the classic antipsychotic drug chlorpromazine [46].…”

Section: Resultscontrasting

confidence: 97%

Modulatory effect of olanzapine on SMIM20/phoenixin, NPQ/spexin and NUCB2/nesfatin-1 gene expressions in the rat brainstem

et al. 2021

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Background Phoenixin, spexin and nesfatin-1 belong to a family of newly discovered multifunctional neuropeptides that play regulatory roles in several brain structures and modulate the activity of important neural networks. However, little is known about their expression and action at the level of brainstem. The present work was, therefore, focused on gene expression of the aforementioned peptides in the brainstem of rats chronically treated with olanzapine, a second generation antipsychotic drug. Methods Studies were carried out on adult, male Sprague–Dawley rats that were divided into 2 groups: control and experimental animals treated with olanzapine (28-day-long intraperitoneal injection, at dose 5 mg/kg daily). All individuals were killed under anesthesia and the brainstem excised. Total mRNA was isolated from homogenized samples of both structures and the RT-PCR method was used for estimation of related SMIM20/phoenixin, NPQ/spexin and NUCB2/nesfatin-1 gene expression. Results Long-term treatment with olanzapine is reflected in qualitatively different changes in expression of examined neuropeptides mRNA in the rat brainstem. Olanzapine significantly decreased NPQ/spexin mRNA expression, but increased SMIM20/phoenixin mRNA level in the rat brainstem; while NUCB2/nesfatin-1 mRNA expression remained unchanged. Conclusions Olanzapine can affect novel peptidergic signaling in the rat brainstem. This may cautiously suggest the presence of an alternative mode of its action.

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Section: Resultscontrasting

confidence: 97%

Modulatory effect of olanzapine on SMIM20/phoenixin, NPQ/spexin and NUCB2/nesfatin-1 gene expressions in the rat brainstem

et al. 2021

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“…Potential effects of antidepressants on the kisspeptin signalling is so far unveiled and there are no articles available dealing with the relations between SSRIs and kisspeptin/Kiss1R expressions in the brain. Our data are only approximate to the study for kisspeptin after chronically administered classical neuroleptics haloperidol and chlorpromazine, where a decrease Kiss-1 expression was noted in the amygdala [ 15 ]. Kisspeptin facilitates hippocampal synaptic transmission by activation of MAP kinase (MAPK) pathway in the granular cells of dentate gyrus.…”

Section: Resultsmentioning

confidence: 64%

Escitalopram as a modulator of proopiomelanocortin, kisspeptin, Kiss1R and MCHR1 gene expressions in the male rat brain

et al. 2020

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Neuropeptides are important, multifunctional regulatory factors of the nervous system, being considered as a novel, atypical sites of antidepressants action. It has already been proven that some of them, such as selective serotonin reuptake inhibitors (SSRI), are able to affect peptidergic pathways in various brain regions. Despite these reports, there is so far no reports regarding the effect of treatment with SSRIs on brain proopiomelanocortin (POMC), kisspeptin, Kiss1R and MCHR1 gene expression. In the current study we examined POMC, kisspeptin, Kiss1R and MCHR1 mRNA expression in the selected brain structures (hypothalamus, hippocampus, amygdala, striatum, cerebellum and brainstem) of rats chronically treated with a 10 mg/kg dose of escitalopram using quantitative Real-Time PCR. Long-term treatment with escitalopram led to the upregulation of MCHR1 expression in the rat amygdala. Kisspeptin mRNA level was also increased in the amygdala, but Kiss1R mRNA expressions were elevated in the hippocampus, hypothalamus and cerebellum. POMC mRNA expressions were in turn decreased in the hippocampus, amygdala, cerebellum and brainstem. These results may support the hypothesis that these neuropeptides may be involved in the site-dependent actions of SSRI antidepressants. This is the first report of the effects of escitalopram on POMC, kisspeptin, Kiss1R and MCHR1 in animal brain. Our findings shed a new light on the pharmacology of SSRIs and may contribute to a better understanding of the alternative, neuropeptide-dependent modes of antidepressant action.

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“…found that chronic i.p. administration with haloperidol and chlorpromazine, the classical antipsychotic drugs, increased the spexin and pomc mRNA levels while decreased the kisspeptin-1 mRNA level in the rat amygdala (Palasz et al., 2018). It indicates that the neuropeptide spexin in the amygdala may be related to the mechanism of the antipsychotic drugs.…”

Section: Pain Anxiety and Depressionmentioning

confidence: 99%

Emerging Roles of NPQ/Spexin in Physiology and Pathology

Lv¹,

Zhou²,

Zhang³

et al. 2019

Front. Pharmacol.

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Spexin (SPX), also called neuropeptide Q (NPQ), is a novel endogenous neuropeptide. Spexin gene and protein are widely expressed in central nervous system and peripheral tissues in humans, rodents, goldfish, etc. A few of physiological and pathological roles of spexin are gradually emerged recently. This article summarized the roles of spexin in feeding behavior, gastrointestinal motility, obesity, diabetes, energy metabolism, endocrine, mental diseases, and cardiovascular function. Given the broad roles of spexin, this neuropeptide has attracted much interest from investigators and will be as a promising future target for novel therapeutic research and drug design.

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Effect of long-term treatment with classical neuroleptics on NPQ/spexin, kisspeptin and POMC mRNA expression in the male rat amygdala

Cited by 9 publications

References 59 publications

Modulatory effect of olanzapine on SMIM20/phoenixin, NPQ/spexin and NUCB2/nesfatin-1 gene expressions in the rat brainstem

Modulatory effect of olanzapine on SMIM20/phoenixin, NPQ/spexin and NUCB2/nesfatin-1 gene expressions in the rat brainstem

Escitalopram as a modulator of proopiomelanocortin, kisspeptin, Kiss1R and MCHR1 gene expressions in the male rat brain

Emerging Roles of NPQ/Spexin in Physiology and Pathology

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