Effect of long-term storage on the quality of cryopreserved sperm of the giant grouper, Epinephelus lanceolatus

Park, Jung Yeol; Zidni, Irfan; Lee, Yun Ho; Lee, Hyo Bin; Lim, Han Kyu

doi:10.1016/j.aquaculture.2022.738154

Cited by 11 publications

(4 citation statements)

References 57 publications

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“…A similar observation was recorded in the case of Pseudopleuronectes yokohamae after 7 days and 1 year of storage of cryopreserved sperm in LN 2 (Hossen et al 2021) . Recently, in giant grouper Park et al (2022) showed sperm cell survival rate after 1 year of storage (91 ± 3%) was similar to fresh sperm (98 ± 0.4%). In this species also, the storage days did not have effect on the sperm quality.…”

Section: Discussionmentioning

confidence: 84%

Simplified cryopreservation of sperm in Grey mullet Mugil cephalus: A robust method for wide adoption

Ramachandran

Kumbha

Saini

et al. 2022

Preprint

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In this study, we developed a simple and robust method of cryopreservation of sperm in Mugil cephalus. A series of experiments were conducted to develop a simplified method by changing the extender, cryoprotectant and freezing height above the liquid nitrogen (LN2) surface. Fresh sperm were diluted 1:1 in a cryomedium (cryomedium = extender + cryoprotectant) containing cryoprotective agents (CPAs) namely, propylene glycol (PG), methanol (MeOH), glycerol (GLY), ethylene glycol (EG), dimethylsulfoxide (DMSO) and dimethylacetamide (DMA). The final concentration of CPAs in cryomedium was 5% and 10% (v/v). The cryovials (2.0 mL) were loaded with diluted samples, fixed in cryo canes, and frozen at 4, 6, 8, 10 and 12 cm above the LN2 surface and also by vitrification. After 7, 30 and 180 days of storage in LN2 (-196 °C), the sperm was thawed at 30 °C for 90-120 s and their quality was evaluated. Among the experimented factors, sperm diluted in cryomedium composed of 0.3 M glucose combined with 10% EG, frozen at 4 cm above the LN2 surface recorded significantly (P < 0.05) highest post-thaw motility (73 ± 2%) and (71 ± 1%) viability respectively. However, no significant (P > 0.05) differences were observed in sperm quality at different storage days. Thus, the results of the study revealed that using this simplified method of cryopreservation high-quality post-thaw sperm can be obtained. Besides, it is expected that this method can be successfully adoptable at hatchery, farm and on-site cryopreservation of sperm in grey mullet.

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Section: Discussionmentioning

confidence: 84%

Simplified cryopreservation of sperm in Grey mullet Mugil cephalus: A robust method for wide adoption

Ramachandran

Kumbha

Saini

et al. 2022

Preprint

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“…Sucrose was the second most effective diluent, after Stein’s solution. The application of sucrose as a diluent has been reported in kelp grouper, Epinephelus moara [ 41 ]; giant grouper [ 11 ]; spotted halibut [ 4 ]; Brazilian flounder [ 23 ]; winter flounder, Pseudopleuronectes americanus [ 42 ]; and summer flounder [ 43 ]. In contrast, the use of Hank’s solution, 300 mM sucrose, and Ringer’s solution resulted in low post-thaw sperm quality.…”

Section: Discussionmentioning

confidence: 99%

“…Using Cell Counting Kit-8 (CCK-8) assays (Bimake, Houston, TX, USA), the survival rates of fresh and thawed sperm cells were evaluated in accordance with a previously described method [ 11 ]. CCK-8 reagent was added directly to cells in culture medium at a volume ratio of 1:10.…”

Section: Methodsmentioning

confidence: 99%

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Cryopreservation of Roughscale Sole (Clidoderma asperrimum) Sperm: Effects of Cryoprotectant, Diluent, Dilution Ratio, and Thawing Temperature

Zidni

Lee

Yoon

et al. 2022

Animals

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The roughscale sole, Clidoderma asperrimum is categorized as an endangered species. Sperm freezing is essential for preserving gametes. This study examined the CPA concentration, diluent, dilution ratio, and thawing temperature to design a sperm cryopreservation protocol for roughscale sole. The variables examined included sperm motility and kinematics, cell survival, fertilization, and DNA fragmentation. Sperm motility parameters were assessed via computer-assisted sperm analysis using a CEROS II instrument. Cell survival rate and DNA damage were assessed using the Cell Counting Kit-8 and single-cell gel electrophoresis assay, respectively. Sperm preservation was tested using several CPAs, including ethylene glycol, dimethyl sulfoxide (DMSO), glycerol, propylene glycol, and methanol. The diluents tested were 300 mM sucrose, 300 mM glucose, Stein’s solution, Ringer’s solution, and Hank’s solution. The optimal conditions for sperm cryopreservation were 10% DMSO + Stein’s solution. After thawing, sperm motility was highest with a 1:1 dilution ratio (sperm to CPA + diluent), at 69.20 ± 0.32%; thawing at 10 °C was optimal for post-thaw motility (72.03 ± 0.95%). The highest fertilization rate (40.00 ± 1.22%) was obtained using DMSO. The fresh sperm had the lowest tail DNA, followed by 10% DMSO + Stein’s solution. The developed cryopreservation methods can be used in roughscale sole hatcheries.

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Shipping duration and temperature influence the characteristics of cryopreserved horse semen stored in different shipping devices for up to 14 days

El-Shalofy

Gautier

Khan

et al. 2023

Animal Reproduction Science

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Effect of long-term storage on the quality of cryopreserved sperm of the giant grouper, Epinephelus lanceolatus

Cited by 11 publications

References 57 publications

Simplified cryopreservation of sperm in Grey mullet Mugil cephalus: A robust method for wide adoption

Simplified cryopreservation of sperm in Grey mullet Mugil cephalus: A robust method for wide adoption

Cryopreservation of Roughscale Sole (Clidoderma asperrimum) Sperm: Effects of Cryoprotectant, Diluent, Dilution Ratio, and Thawing Temperature

Shipping duration and temperature influence the characteristics of cryopreserved horse semen stored in different shipping devices for up to 14 days

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