Effect of long-term plant biomass management on phosphatase-producing bacterial populations in soils under temperate grassland

Gaiero, Jonathan R.; Bent, Elizabeth; Boitt, Gustavo; Condron, Leo M.; Dunfield, Kari E.

doi:10.1016/j.apsoil.2020.103583

Cited by 6 publications

(10 citation statements)

References 62 publications

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“…total P) and stoichiometry (e.g. C:N, C:P, N:P) were found to be predictors of community diversity and composition or abundance of class A acid phosphatase communities (Luo et al 2019;Zheng et al 2019;Gaiero et al 2020). phoDharbouring communities may also respond uniquely to resource limitations and available P resources (Jorquera et al 2014;Margalef et al 2017;Gaiero et al 2020).…”

Section: Microbially-driven P Cyclingmentioning

confidence: 97%

“…Total gene copies and total transcripts (cDNA) for bacteria (16S rRNA), fungi (18S rRNA), and bacterial genes relating to organic P cycling were quantified when possible. Bacterial nonspecific acid phosphatase genes (class A: phoC/phoN, and class C: olpA/lppC) and transcripts were quantified as previously described (Fraser et al 2017;Gaiero et al 2018Gaiero et al , 2020 S1).…”

Section: Quantitative Pcrmentioning

confidence: 99%

“…(class A, phoN/phoC) and alkaline phosphatase (phoD) genes were generated, and sequenced on the MiSeq platform (Genomics Facility at the University of Guelph) as previously outlined (Gaiero et al 2020) (Supplemental Table S2). Sequence reads for class A were clustered at an 88% sequence similarity (Gaiero et al 2020), and at 75% for phoD (Tan et al 2013). There was a total of 3,998,784 reads resulting in 356 operational taxonomic units (OTUs) for class A, and 94,758 reads resulting in 276 OTUs for phoD.…”

Section: P-cycling Genesmentioning

confidence: 99%

“…There was a total of 3,998,784 reads resulting in 356 operational taxonomic units (OTUs) for class A, and 94,758 reads resulting in 276 OTUs for phoD. For the non-specific acid (class A, phoN/phoC) and alkaline (phoD) phosphatase genes, specificity and taxonomic assignment of OTUs was performed using BLASTx (Altschul 1997), with an E-value cutoff of 1e -10 to the closest matching bacterial order (Gaiero et al 2020). Phylogenetic trees were generated online using the pipeline available at http://www.phylogeny.fr/ (Dereeper et al 2008).…”

Section: P-cycling Genesmentioning

confidence: 99%

“…C:N, C:P, N:P) were found to be predictors of community diversity and composition or abundance of class A acid phosphatase communities (Luo et al 2019;Zheng et al 2019;Gaiero et al 2020). phoDharbouring communities may also respond uniquely to resource limitations and available P resources (Jorquera et al 2014;Margalef et al 2017;Gaiero et al 2020). Admittedly, the abundance and diversity of bacterial phosphatases and their drivers is complex (Spohn and Kuzyakov 2013;Margalef et al 2017;Neal et al 2018;Gaiero et al 2020), and there is still a need to better understand their biogeography and ecological roles (George et al 2018).…”

Section: Microbially-driven P Cyclingmentioning

confidence: 99%

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Soil microbial communities influencing organic phosphorus mineralization in a coastal dune chronosequence in New Zealand

Gaiero

Tosi

Bent

et al. 2021

FEMS Microbiology Ecology

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The Haast chronosequence in New Zealand is a ∼6500-year dune formation series, characterized by rapid podzol development, phosphorus (P) depletion, and a decline in aboveground biomass. We examined bacterial and fungal community composition within mineral soil fractions using amplicon-based high-throughput sequencing (Illumina MiSeq). We targeted bacterial non-specific acid (class A, phoN/phoC) and alkaline (phoD) phosphomonoesterase genes and quantified specific genes and transcripts using real-time PCR. Soil bacterial diversity was greatest after 4000 years of ecosystem development and associated with an increased richness of phylotypes and a significant decline in previously dominant taxa (Firmicutes and Proteobacteria). Soil fungal communities transitioned from predominantly Basidiomycota to Ascomycota along the chronosequence and were most diverse in 290 to 392-year-old soils, coinciding with maximum tree basal area and organic P accumulation. The Bacteria: Fungi ratio decreased amid a competitive and interconnected soil community as determined by network analysis. Overall, soil microbial communities were associated with soil changes and declining P throughout pedogenesis and ecosystem succession. We identified an increased dependence on organic P mineralization, as found by the profiled acid phosphatase genes, soil acid phosphatase activity, and function inference from predicted metagenomes (PICRUSt2).

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Section: Microbially-driven P Cyclingmentioning

confidence: 97%

Section: Quantitative Pcrmentioning

confidence: 99%

Section: P-cycling Genesmentioning

confidence: 99%

Section: P-cycling Genesmentioning

confidence: 99%

Section: Microbially-driven P Cyclingmentioning

confidence: 99%

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Soil microbial communities influencing organic phosphorus mineralization in a coastal dune chronosequence in New Zealand

Gaiero

Tosi

Bent

et al. 2021

FEMS Microbiology Ecology

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Microbial Genes, Enzymes, and Metabolites: To Improve Rhizosphere and Plant Health Management

Fazeli‐Nasab

Rahmani

2021

Microbiological Activity for Soil and Plant Health Management

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Differential structure and function of phosphorus‐mineralizing microbial communities in organic and upper mineral soil horizons across a temperate rainforest chronosequence

Dunfield,

Mitter,

Richardson

et al. 2024

Environmental Microbiology

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Microbial community structure and function were assessed in the organic and upper mineral soil across a ~4000‐year dune‐based chronosequence at Big Bay, New Zealand, where total P declined and the proportional contribution of organic soil in the profile increased with time. We hypothesized that the organic and mineral soils would show divergent community evolution over time with a greater dependency on the functionality of phosphatase genes in the organic soil layer as it developed. The structure of bacterial, fungal, and phosphatase‐harbouring communities was examined in both horizons across 3 dunes using amplicon sequencing, network analysis, and qPCR. The soils showed a decline in pH and total phosphorus (P) over time with an increase in phosphatase activity. The organic horizon had a wider diversity of Class A (phoN/phoC) and phoD‐harbouring communities and a more complex microbiome, with hub taxa that correlated with P. Bacterial diversity declined in both horizons over time, with enrichment of Planctomycetes and Acidobacteria. More complex fungal communities were evident in the youngest dune, transitioning to a dominance of Ascomycota in both soil horizons. Higher phosphatase activity in older dunes was driven by less diverse P‐mineralizing communities, especially in the organic horizon.

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Effect of long-term plant biomass management on phosphatase-producing bacterial populations in soils under temperate grassland

Cited by 6 publications

References 62 publications

Soil microbial communities influencing organic phosphorus mineralization in a coastal dune chronosequence in New Zealand

Soil microbial communities influencing organic phosphorus mineralization in a coastal dune chronosequence in New Zealand

Microbial Genes, Enzymes, and Metabolites: To Improve Rhizosphere and Plant Health Management

Differential structure and function of phosphorus‐mineralizing microbial communities in organic and upper mineral soil horizons across a temperate rainforest chronosequence

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