Effect of long non-coding RNA AOC4P on gastrointestinal stromal tumor cells

Abstract

ObjectiveIn this research, we explored the effect of long non-coding RNA (lncRNA) AOC4P on gastrointestinal stromal tumor (GIST) cells.Materials and methodsThe expression of lncRNA AOC4P in tissues was detected by real-time PCR (RT-PCR). The epithelial–mesenchymal transition (EMT)-related proteins in tissues were analyzed by Western blot. The experiment included negative control group (CN), silence AOC4P group (si AOC4P), and silence negative control group (si CT). RT-PCR, MTT, Scratch, Transwell, and Annexin V-FITC methods were used to detect the expression of lncRNA AOC4P, cell proliferation, cell migration ability, cell invasion ability, and apoptosis, respectively. The EMT-related proteins including TGF-β, ZEB1, Vimentin, Snail, and E-cadherin were analyzed by Western blot.ResultsThe expression of lncRNA AOC4P and the expression of EMT-related proteins in high-risk GISTs were higher than that in low- and intermediate-risk GISTs (P<0.05). It was revealed that cell proliferative migration and invasive ability in si AOC4P group was decreased than that in CN and si CT groups (P<0.05), and cell apoptosis in si AOC4P group was higher than that in si CT group. The results of Western blot demonstrated that the expression of TGF-β1, ZEB1, Vimentin, and Snail in si AOC4P group were lower than that in si CT and CN group (P<0.05), and the expression of E-cadherin in si AOC4P group was higher than that in si CT and CN group (P<0.05).

Finding associations in a heterogeneous setting: Statistical test for aberration enrichment

“…RASSF4 is a member of the RASSF family of tumor suppressors. AOC4P is a lncRNA involved in Hepatocellular Carcinoma and Colorectal Cancer [22] and ADHFE1 is a breast cancer oncogene [41].…”

Deficiency of pseudogene UPAT leads to hepatocellular carcinoma progression and forms a positive feedback loop with ZEB1

“…The exact roles of UPAT in the EMT process is divergent in cancer types 15‐17 . Based on bioinformatics analysis and the correlation between UPAT expression and MVI, we preliminarily discovered that UPAT not only inhibits the EMT process, but also controls the acquisition of CSC properties by suppressing the expression of CSC‐related TFs and markers.…”

“…Taniue et al 14 first reported the oncogenic effect of UPAT in colon tumorigenesis by inhibiting TrCP‐mediated UHRF1 degradation but, unfortunately, UPAT expression was not evaluated in colon cancer tissues. In gastric cancer and gastrointestinal stromal tumors, UPAT was thought to be upregulated in tumor tissues and positively correlated with greater proliferative, migrative, and invasive abilities, as well as the transition from epithelial to mesenchymal states 15,16 . Another study, however, reported that UPAT was significantly downregulated in HCC and negatively correlated with worse clinicopathological parameters.…”

Integrated Analysis of Long Non-Coding RNAs and mRNAs Associated With Malignant Transformation of Gastrointestinal Stromal Tumors

“…LncRNAs have been shown to play important roles in the regulation of various biological processes and the development of various diseases [25]. Several lncRNAs identi ed in other malignant tumors, such as HOTAIR, CCDC26, and AOC4P, have also been evaluated in GISTs and were shown to be associated with GIST metastasis and sensitivity to imatinib [17,26,27]. Gyvyte et al performed a next-generation sequencing study of paired GISTs and adjacent tissue samples from 15 patients and identi ed two deregulated lncRNAs (H19 and FENDRR) in GISTs [28].…”